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dc.contributor.authorSanz, Juan Carlos
dc.contributor.authorOry, Fernando de 
dc.contributor.authorGuisasola, Maria Eulalia 
dc.contributor.authorBalfagon, Pilar 
dc.date.accessioned2020-06-16T08:32:22Z
dc.date.available2020-06-16T08:32:22Z
dc.date.issued2018-01
dc.identifier.citationJ Clin Lab Anal . 2018 Jan;32(1):e22203.es_ES
dc.identifier.issn0887-8013es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/10445
dc.description.abstractSerology for type-specific herpes simplex virus (HSV) is based on the use of the respective glycoprotein G (gG). Chemiluminescent immunoassay (CLIA; BIO-FLASH® , Biokit, Spain), ELISA (HerpeSelect® , Focus, USA), and immunoblot (IB; Virotech, Germany) for detecting HSV-1- and HSV-2-specific IgG were compared using 384 serum samples received for HSV serology. The samples were classified as positive or negative according to a consensus criterion. For HSV-1, 262 samples were positive and 118 were negative (four samples were unclassifiable). IB showed agreement, sensitivity, and specificity values of 98.68%, 98.47% and 99.15%, respectively. The corresponding figures for CLIA and ELISA were 98.95%, 99.24% and 98.31%, and 98.16%, 99.62% and 94.92%, respectively. For HSV-2, 106 samples were positive and 278 were negative. Agreement, sensitivity, and specificity of IB were 99.48%, 98.11%, and 100%, respectively. The corresponding figures for CLIA and ELISA were 99.48%, 99.06% and 99.64%, and 98.18%, 99.06% and 97.84%, respectively. The three methods showed excellent and equivalent performance characteristics for the detection of type-specific IgG to HSV-1 and HSV-2.es_ES
dc.description.sponsorshipThis work was supported in part by a contract between Biokit Research and Development SLU and the Institute of Health Carlos III (MVP1172/13).es_ES
dc.language.isoenges_ES
dc.publisherWileyes_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshSimplexvirus es_ES
dc.subject.meshAntibodies, Viral es_ES
dc.subject.meshEnzyme-Linked Immunosorbent Assay es_ES
dc.subject.meshHerpes Simplex es_ES
dc.subject.meshHumans es_ES
dc.subject.meshImmunoblotting es_ES
dc.subject.meshImmunoglobulin G es_ES
dc.subject.meshLuminescent Measurements es_ES
dc.titleComparison of commercial methods of immunoblot, ELISA, and chemiluminescent immunoassay for detecting type-specific herpes simplex viruses-1 and -2 IgG.es_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID28332725es_ES
dc.format.volume32es_ES
dc.format.number1es_ES
dc.format.pagee22203es_ES
dc.identifier.doi10.1002/jcla.22203es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1098-2825es_ES
dc.relation.publisherversionhttps://doi.org/10.1002/jcla.22203es_ES
dc.identifier.journalJournal of clinical laboratory analysises_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/MVP1172/13es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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