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dc.contributor.authorDíez-Martínez, Roberto
dc.contributor.authorGarcía-Fernández, Esther
dc.contributor.authorLetrado, Patricia
dc.contributor.authorGarcía, Pedro
dc.contributor.authorCorsini, Bruno 
dc.contributor.authorAguinagalde, Leire 
dc.contributor.authorGonzalez-Camacho, Fernando 
dc.contributor.authorYuste, Jose Enrique
dc.identifier.citationAntimicrob Agents Chemother . 2018 May 25;62(6):e02212-17.es_ES
dc.description.abstractBacteriophage-borne lytic enzymes, also named lysins or enzybiotics, are efficient agents for the killing of bacterial pathogens. The colonization of the respiratory tract by Streptococcus pneumoniae is a prerequisite for the establishment of the infection process. Hence, we have evaluated the antibacterial activities of three different lysins against pneumococcal colonization using human nasopharyngeal and lung epithelial cells as well as a mouse model of nasopharyngeal colonization. The lysins tested were the wild-type Cpl-1, the engineered Cpl-7S, and the chimera Cpl-711. Moreover, we included amoxicillin as a comparator antibiotic. Human epithelial cells were infected with three different multidrug-resistant clinical isolates of S. pneumoniae followed by a single dose of the corresponding lysin. The antimicrobial activities of these lysins were also evaluated using a mouse nasopharyngeal carriage model. The exposure of the infected epithelial cells to Cpl-7S did not result in the killing of any of the pneumococcal strains investigated. However, the treatment with Cpl-1 or Cpl-711 increased the killing of S. pneumoniae organisms adhered to both types of human epithelial cells, with Cpl-711 being more effective than Cpl-1, at subinhibitory concentrations. In addition, a treatment with amoxicillin had no effect on reducing the carrier state, whereas mice treated by the intranasal route with Cpl-711 showed significantly reduced nasopharyngeal colonization, with no detection of bacterial load in 20 to 40% of the mice. This study indicates that Cpl-1 and Cpl-711 lysins might be promising antimicrobial candidates for therapy against pneumococcal colonization.es_ES
dc.description.sponsorshipFunding for this research was provided by: Ministerio de Economía y Competitividad (SAF2017-83388, SAF2017-88664)es_ES
dc.publisherAmerican Society for Microbiology (ASM) es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAnti-Bacterial Agents es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMice es_ES
dc.subject.meshNasopharyngeal Diseases es_ES
dc.subject.meshPneumococcal Infections es_ES
dc.subject.meshRespiratory System es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.titleChemotherapy with Phage Lysins Reduces Pneumococcal Colonization of the Respiratory Tract.es_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.identifier.journalAntimicrobial agents and chemotherapyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.rights.accessRightsopen accesses_ES

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