Browsing by MeSH term "DNA Fingerprinting"
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Publication DNA degradation in human teeth exposed to thermal stress(Nature Publishing Group, 2021-06-09) Lozano-Peral, Diego; Rubio, Leticia; Santos, Ignacio; Gaitán, María Jesús; Viguera, Enrique; Martín-de-Las-Heras, Stella; [Lozano-Peral,D; Rubio,L; Santos,I; Gaitán,MJ; Martín-de-Las-Heras,S] Department of Forensic Dentistry and Medicine, Instituto de Investigación Biomédica de Málaga-IBIMA (CE-18), School of Medicine, University of Malaga, Malaga, Spain. [Lozano-Peral.D] Supercomputing and Bioinnovation Center, Servicios Centrales de Apoyo a la Investigación, University of Malaga, Malaga, Spain. [Viguera,E] Department of Cellular Biology, Genetics and Physiology, University of Malaga, Malaga, Spain. [Lozano-Peral,D; Rubio,L] These authors contributed equally: Diego Lozano-Peral and Leticia RubioHuman identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.Publication Epidemiological tracing of Salmonella enterica serotype Abortusovis from Spanish ovine flocks by PFGE fingerprinting(Cambridge University Press, 2007-05) Valdezate, Sylvia; Astorga, R; Herrera-León, Silvia; Perea, A; Usera, Miguel A.; Huerta, B; Echeita, Aurora; Instituto de Salud Carlos III; Regional Government of Andalusia (España)Salmonella enterica serotype Abortusovis, an ovine host-specific serotype, rare in most countries, is responsible for epidemic abortion episodes in Spain. With the aim of surveillance and detection of the spread of specific clones, 55 Abortusovis isolates collected during 1996-2001 from flocks in 11 provinces, were studied using XbaI-PFGE. Despite the fact that the strains were geographically and spatially related, PFGE demonstrated an epidemiologically acceptable discriminating power, identifying 20 clones (similarity, 52-96%). Clones Sabv6, 1, 5 were disseminated in seven, five and two areas respectively, while another 17 clones appeared in single places. Clones from nearby geographic regions showed a high relatedness (one band of difference in the PFGE profile) Sabv1-2-3, Sabv5-6, Sabv7-8, and Sabv13-14, suggesting a common ancestor. Co-isolation in the same flock (Sabv5-6, Sabv1-3, Sabv1-6) was detected. PFGE surveillance detected the predominance and widespread distribution of clone Sabv6 in 21 out of the 55 Abortusovis serotype episodes studied in Spain.