Browsing by Keyword "Chondrogenesis"
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Publication Synergistic Effects of a Mixture of Glycosaminoglycans to Inhibit Adipogenesis and Enhance Chondrocyte Features in Multipotent Cells(Karger Publishers, 2015) Petrov, Petar D.; Granados, Nuria; Chetrit, Carles; Martinez-Puig, Daniel; Palou, Andreu; Luisa Bonet, M.Background/Aims: Multipotent mesenchymal stem cells affect homeostasis of adipose and joint tissues. Factors influencing their differentiation fate are of interest for both obesity and joint problems. We studied the impact of a mixture of glycosaminoglycans (GAGs) (hyaluronic acid: dermatan sulfate 1:0.25, w/w) used in an oral supplement for joint discomfort (OralviscTM) on the differentiation fate of multipotent cells. Methods: Primary mouse embryo fibroblasts (MEFs) were used as a model system. Post-confluent monolayer MEF cultures non-stimulated or hormonally stimulated to adipogenesis were chronically exposed to the GAGs mixture, its individual components or vehicle. The appearance of lipid laden cells, lipid accumulation and expression of selected genes at the mRNA and protein level was assessed. Results: Exposure to the GAGs mixture synergistically suppressed spontaneous adipogenesis and induced the expression of cartilage extracellular matrix proteins, aggrecan core protein, decorin and cartilage oligomeric matrix protein. Hormonally-induced adipogenesis in the presence of the GAGs mixture resulted in decreased ad ipogenic differentiation, down-regulation of ad ipogenic/ lipogenic factors and genes for insulin resistance-related adipokines (resistin and retinol binding protein 4), and up-regulation of oxidative metabolism-related genes. Adipogenesis in the presence of dermatan sulfate, the minor component of the mixture, was not impaired but resulted in smaller lipid droplets and the induction of a more complete brown adipocyterelated transcriptional program in the cells in the adipose state. Conclusions: The Oralviscm GAGs mixture can tip the adipogenic/chondrogenic fate balance of multipotent cells away from adipogenesis while favoring chondrocyte related gene expression. The mixture and its dermatan sulfate component also have modulatory effects of interest on hormonally-induced adipogenesis and on metabolic and secretory capabilities of adipose cells.Publication The Janus Role of Adhesion in Chondrogenesis(Multidisciplinary Digital Publishing Institute (MDPI), 2020-07-24) Casanellas, Ignasi; Lagunas, Anna; Vida, Yolanda; Pérez-Inestrosa, Ezequiel; Andrades, José A.; Becerra, José; Samitier, Josep; [Casanellas,I; Lagunas,L; Samitier,J] Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain. [Casanellas,I; Samitier,J] Department of Electronics and Biomedical Engineering, University of Barcelona (UB), Barcelona, Spain. [Casanellas,I; Lagunas,L; Andrades,JA; Samitier,J; Becerra,J; Samitier,J] Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain. [Vida,Y; Pérez-Inestrosa,E] Departamento de Química Orgánica, Universidad de Málaga-IBIMA, Málaga, Spain. [Vida,Y; Pérez-Inestrosa,E; Becerra,J] Centro Andaluz de Nanomedicina y Biotecnología-BIONAND, Campanillas, Málaga, Spain. [Andrades,JA; Becerra,J] Department of Cell Biology, Genetics and Physiology, Universidad de Málaga-IBIMA, Málaga, Spain.Tackling the first stages of the chondrogenic commitment is essential to drive chondrogenic differentiation to healthy hyaline cartilage and minimize hypertrophy. During chondrogenesis, the extracellular matrix continuously evolves, adapting to the tissue adhesive requirements at each stage. Here, we take advantage of previously developed nanopatterns, in which local surface adhesiveness can be precisely tuned, to investigate its effects on prechondrogenic condensation. Fluorescence live cell imaging, immunostaining, confocal microscopy and PCR analysis are used to follow the condensation process on the nanopatterns. Cell tracking parameters, condensate morphology, cell-cell interactions, mechanotransduction and chondrogenic commitment are evaluated in response to local surface adhesiveness. Results show that only condensates on the nanopatterns of high local surface adhesiveness are stable in culture and able to enter the chondrogenic pathway, thus highlighting the importance of controlling cell-substrate adhesion in the tissue engineering strategies for cartilage repair.Publication Validation of the 1,4-butanediol thermoplastic polyurethane as a novel material for 3D bioprinting applications(Wiley, 2020-10-20) Chocarro-Wrona, Carlos; de Vicente, Juan; Antich, Cristina; Jiménez, Gema; Martínez-Moreno, Daniel; Carrillo, Esmeralda; Montañez, Elvira; Gálvez-Martín, Patricia; Perán, Macarena; López-Ruiz, Elena; Marchal, Juan Antonio; [Chocarro-Wrona,C; Antich,C; Jiménez,G; Martínez-Moreno,D; Carrillo,E; Perán,M; López-Ruiz,E; Marchal,JA] Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain. [Chocarro-Wrona,C; Antich,C; Jiménez,G; Martínez-Moreno,D; Carrillo,E; Perán,M; López-Ruiz,E; Marchal,JA] Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain. [Chocarro-Wrona,C; Antich,C; Martínez-Moreno,D; Carrillo,E; Marchal,JA] Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada, Spain. [Chocarro-Wrona,C; de Vicente,J; Antich,C; Jiménez,G; Martínez-Moreno,D; Carrillo,E; Perán,M; López-Ruiz,E; Marchal,JA] Excellence Research Unit “Modeling Nature” (MNat), University of Granada, Granada, Spain. [de Vicente,J] Department of Applied Physics, Faculty of Sciences, University of Granada, Granada, Spain. [Montañez,E] Biomedical Research Institute of Málaga (IBIMA), Málaga. [Montañez,E] Department of Orthopedic Surgery and Traumatology, Virgen de la Victoria University Hospital, Málaga, Spain. [Gálvez-Martín,P] Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Granada, Spain. [Gálvez-Martín,P] Advanced Therapies Area, Bioibérica S.A.U, Barcelona, Spain. [Perán,M; López-Ruiz,E] Department of Health Sciences, University of Jaén, Jaén, SpainTissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires the development of functional personalized implants with cartilage-like mechanical properties capable of sustaining high load-bearing environments to integrate into the surrounding tissue of the cartilage defect. In this study, we evaluated the novel 1,4-butanediol thermoplastic polyurethane elastomer (b-TPUe) derivative filament as a 3D bioprinting material with application in cartilage TE. The mechanical behavior of b-TPUe in terms of friction and elasticity were examined and compared with human articular cartilage, PCL, and PLA. Moreover, infrapatellar fat pad-derived human mesenchymal stem cells (MSCs) were bioprinted together with scaffolds. in vitro cytotoxicity, proliferative potential, cell viability, and chondrogenic differentiation were analyzed by Alamar blue assay, SEM, confocal microscopy, and RT-qPCR. Moreover, in vivo biocompatibility and host integration were analyzed. b-TPUe demonstrated a much closer compression and shear behavior to native cartilage than PCL and PLA, as well as closer tribological properties to cartilage. Moreover, b-TPUe bioprinted scaffolds were able to maintain proper proliferative potential, cell viability, and supported MSCs chondrogenesis. Finally, in vivo studies revealed no toxic effects 21 days after scaffolds implantation, extracellular matrix deposition and integration within the surrounding tissue. This is the first study that validates the biocompatibility of b-TPUe for 3D bioprinting. Our findings indicate that this biomaterial can be exploited for the automated biofabrication of artificial tissues with tailorable mechanical properties including the great potential for cartilage TE applications.