S1 SUPPLEMENTARY MATERIAL Proteomic analysis of plasma proteins of high-flux haemodialysis and on-line haemodiafiltration patients reveals differences in transthyretin levels related with anaemia Emma Martínez-Alonso,1,2 Paula Alcázar,1 Emilio Camafeita,3,4 Milagros Fernández- Lucas,5,6 Gloria Ruíz-Roso,5 and Alberto Alcázar 1,2* 1 Proteomics Unit. Hospital Universitario Ramón y Cajal, ,5<&,6Madrid, Spain 2 Department of Research, Hospital Universitario Ramón y Cajal, IRYCIS, Madrid, Spain 3 Proteomics Unit. Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid, Spain 4 Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Spain 5 Department of Nephrology, Hospital Universitario Ramón y Cajal, IRYCIS, Madrid, Spain 6 Department of Nephrology, Facultad de Medicina, Universidad de Alcalá$OFDOiGH+HQDUHV 6SDLQ  Table of Contents: 1. High-flux haemodialysis and on-line haemodiafiltration……………………….......S2 2. Baseline characteristics of the patients……………………………………………...S3 3. Preparation of plasma samples………………………………………………………S3 4. 1-D DIGE analysis..………………..…………………...…………………………...S4 4.1. Fluorescence labelling. DIGE assay..…….…………………….…………..S4 4.2. In-gel protein digestion………………………….………….……………....S4 4.3. Protein identification by mass spectrometry……………………..……........S5 5. LC-MS/MS Analysis………………………………………………………...………S5 5.1 Protein digestion…………………………………………………………….S5 5.2. Isobaric labelling of peptides using tandem tass tags (TMT)……………....S6 5.3. Peptide identification…………………………………………………….…S6 5.4. Quantification at the peptide and protein levels…..………………………..S7 6. Quantification of TTR peptides by mass spectrometry. Calibration experiments…...S8 S2 7. Detection of the allele distribution of haptoglobin α in HF and HDF patients………S9 8. Supplementary Table S1……………………………………………………..……....S10 9. Supplementary Table S2……………………………………………………..……....S11 10. Supplementary Figure S1……………………………………………….…….....…..S15 11. Supplementary Figure S2…………………………………………………….…..….S16 12. Supplementary Figure S3…………………………………………………….…..….S17 13. Supplementary Figure S4…………………………………………………...….…....S19 14. Supplementary Figure S5……………………………………………………………S20 15. Supplementary Figure S6……………………………………………………………S21 16. MALDI-TOF MS spectra and Mascot searches……………………………….…….S22 17. MALDI LIF-TOF/TOF MS spectra and Mascot searches……………………….….S52 1. High-flux haemodialysis and on-line haemodiafiltration Haemodialysis was prescribed as four-hour dialysis sessions trice weekly, with a blood flow rate between 220 and 400 ml/min. High-flux haemodialysis was performed using dialysis membranes of a blend of polyarylethersulfone and polyvinylpyrrolidone (Revaclear), and polyamide (Polyflux-210H). On-line haemodiafiltration was performed using polysulfone membranes (Helixone). Other aspects of the patient’s treatment prescription did not differ between the two groups. All patients had not residual renal function and were on dialysis for more than 6 months. The treatment times and blood flow rates (individualized for each patient) remained unchanged from their usual prescription. To achieve anticoagulation, heparin was used (firstly a loading dose and then a constant infusion of heparin). Net fluid removal was also individualized. The efficacy of haemodialysis was indexed by the urea kinetic equation single-pool variable volume Kt/V according to Daugirdas [1]. Dialysis vintage years were similar for both groups. Residual renal function was negligible. Kt/V represents the fractional clearance of urea, and it is used as an index of the dialysis dose (K: rate of urea clearance by the dialyzer in ml/min, t: duration of treatment session in min, V: urea volume distribution in the patient in ml). Both groups met the recommendations from the National Kidney Foundation guidelines, Kt/V= 1.2 per haemodialysis session for patients treated thrice weekly [2]. References: S3 1. Daugirdas JT: Second generation logarithmic estimates of single-pool variable volume Kt/V: an analysis of error. J Am Soc Nephrol, 4: 1205-1213, 1993 2. National Kidney Foundation NK: I. NKF-K/DOQI Clinical Practice Guidelines for Haemodialysis Adequacy: update 2000. Am J Kidney Dis, 37: S7-S64, 2001 2. Baseline characteristics of the patients The aetiologies of the ESRD in the patients from both groups were balanced between the groups and representative of the most common causes of ESRD. In the HF group, there were patients suffering from diabetes mellitus (1 patient), glomerulonephritis (2 patient), polycystic kidney disease (1 patient), nephroangiosclerosis and renal ischemia (1 patient), interstitial nephritis (2 patient), multiple myeloma (1 patient), amyloidosis (1 patient) and glomerular hyperfiltration (1 patient). The renal diseases in the HDF group were diabetes mellitus (1 patient), glomerulonephritis (1 patient), polycystic kidney disease (1 patient), nephroangiosclerosis and renal ischemia (1 patient), interstitial nephritis (2 patient), amyloidosis (1 patient), bilateral nephrectomy (1 patient) and unknown (1 patient). In the HDF group there was a patient who had undergone a bilateral nephrectomy due to a renal carcinoma; and a patient whose nephropathy was undiagnosed. All the patients had a glomerular filtration rate (GFR) < 6 ml/ min (end-stage kidney disease, chronic kidney disease stage 5, GFR< 15 ml/min). There were two patients with diabetes mellitus (requiring the administration of insulin) in each group. The Charlson Comorbidity Index (CCI) was used to assess the base comorbidity of these patients. The CCI considers 17 comorbidities and subcategories for liver disease and diabetes mellitus (Roffman 2016). Patients from both groups received subcutaneous recombinant human erythropoietin (rhEPO) weekly. Though, the requirements of erythropoietin varied significantly between both groups. Patients were also prescribed iron (1-2 vials of 1 g, monthly), folic acid (50 mg) and vitamin D (individualized dosage) supplementation. 3. Preparation of plasma samples The patient’s blood sample was drawn after the long interdialytic period in EDTA-K3 tubes. The samples were then centrifuged at 900g for 10 minutes and the plasma was separated. Aliquots were stored at -80°C. To study plasma proteome, purification of pooled or individual plasma samples was performed removing high abundant proteins to improve the resolution of gels and detection of low molecular weight proteins using the Pure Proteome™ Albumin/IgG Depletion kit (Millipore), as recommended by the supplier. Plasma sample corresponding to S4 each patient was stored separately at -80°C, and protein concentrations were determined for each sample. To check the depletion of these proteins, both the unprocessed and the albumin/IgG-depleted plasma samples were run into SDS-PAGE, and the gel was stained with Coomassie blue for protein detection. Supplemental Figure S1 shows the effective depletion of albumin/IgGs in HF and HDF pooled plasma samples compared to unprocessed HF and HDF pooled plasma samples. 4. 1-D DIGE analysis 4.1. Fluorescence labelling. DIGE assay Plasma samples from HF and HDF patients were analyzed by one-dimensional fluorescence difference in gel electrophoresis (1-D DIGE). Albumin/IgG-depleted HF and HDF patients’ plasma samples (5 μg of protein) were made to 8.5 M urea, centrifuged and labelled on cysteine residues with Cy3 or Cy5 fluorescent dyes (GE Healthcare). After dithiothreitol addition, HF and HDF samples were combined and fluorescence-labelled proteins loaded into standard vertical slab SDS-PAGE for 1-D DIGE. SDS-PAGE was performed in 12% acrylamide (3% cross-linking) gels (1.0 mm thick). The gel was scanned using a Typhoon 9200 imager (GE Healthcare) at 450 V per channel, and the paired Cy5/Cy3 images captured at 100 m/pixel resolution for analysis. Cy5 was excited by the laser red-line (633 nm), and Cy3 was excited by the laser green-line (532 nm), sequentially and using appropriate emission filters (Cy5, 670 band-pass 30; Cy3, 580 band-pass 30) to minimize crosstalk. After scanning, the gel was stained with Coomassie blue. 4.2. In-gel protein digestion Firstly, excised stained gel bands were destaining adding 200 l of 50% ethanol (vol/vol) in 25 mM ammonium bicarbonate (AB) for 20 min, and after it was removed, added 200 l of pure ethanol for 15 min, removed it and dried for 5 min. After, in-gel reduction and alkylation of proteins was carried out covering the bands with 200 μl 10 mM dithiothreitol (DTT) in 50 mM AB for 30 min at 56 °C, and after DTT was removed, washed with 50 mM AB for 5 min and bands incubated with 55 mM iodoacetamide in 50 mM AB for 30 min in the dark. Then, iodoacetamide was removed and washed twice with 50 and 25 mM AB for 5 min. Gel pieces were dehydrated adding 200 l of 50% acetonitrile (ACN) (vol/vol) in 25 mM AB for 20 min and removed, it was repeated again, then 200 l of neat ACN were added for 10 min, and S5 after ACN removal, gel pieces were dried completely. Then, the gel pieces were saturated with trypsin adding 15 μl of a solution containing 40 ng of trypsin (1:20 w/w) in 25 mM AB and leaving them in an ice bucket or a fridge for 45 min. After removing liquid excess, 20 l of 25 mM AB was added and incubated overnight at 30°C. Finally, to extract the peptide digestion products, 1 l of neat ACN were added (final concentration 6-15% ACN), incubated at 37°C for 15 min, and then 0.2 l of trifluoroacetic (TFA) was added to 0.1% (vol/vol) final concentration and incubated for 10 min at room temperature, collecting the supernatant for mass spectrometry analysis. 4.3. Protein identification by mass spectrometry After digestion, peptide mass fingerprinting (PMF) was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Autoflex III TOF/TOF, Bruker-Daltonics) for protein identification, as previously described [3]. PMF spectra were obtained using an alpha-cyanocinnamic acid matrix and calibrated with a peptide calibration standard mixture (222570, Bruker Daltonics) contained nine standard peptides in a molecular mass range between ~ 700 and 3500 Da to reach a typical mass measurement accuracy < 3 ppm. Additionally, when available and for confirmation of protein identity, peptide fragmentation was performed by MS in tandem MALDI LIFT-TOF/TOF [4]. MS data of PMFs and MS/MS data from LIFT TOF/TOF spectra were searched in the SwissProt database using the MASCOT database search algorithm (Matrix Science) for protein identification. No more than one missed tryptic cleavage was allowed and a mass accuracy of 50 ppm was used for all mass searches. References: 3. Cid C, Garcia-Bonilla L, Camafeita E, Burda J, Salinas M, Alcazar A: Proteomic characterization of protein phosphatase 1 complexes in ischemia-reperfusion and ischemic tolerance. Proteomics, 7: 3207-3218, 2007 4. Suckau D, Resemann A, Schuerenberg M, Hufnagel P, Franzen J, Holle A: A novel MALDI LIFT- TOF/TOF mass spectrometer for proteomics. Anal Bioanal Chem, 376: 952-965, 2003 5. LC-MS/MS Analysis 5.1 Protein digestion Plasma samples were boiled for 5 min at 100 ºC in the presence of 25 mM Tris-HCl pH 6.8, 1% SDS and 50 mM DTT. This mixture (6 l) was diluted with denaturing buffer (8 M urea S6 in 100 mM Tris-HCl pH 8.5) and concentrated on FASP filters (Expedeon). After washing with denaturing buffer at 10,000 rpm for 15 min, free thiol groups were alkylated by incubation with 50 mM iodoacetamide 30 min at room temperature in the dark. Then the filters were washed with denaturing buffer followed by washing with trypsin digestion buffer (50 mM ammonium bicarbonate pH 8.8). Protein samples were digested overnight at 37ºC with sequencing grade trypsin (Promega, Madison, WI, USA) at 1:40 (w/w) trypsin:protein ratio in digestion buffer. The resulting tryptic peptides from each sample were recovered by centrifugation at 10,000 rpm for 5 min after addition of 40 l of trypsin digestion buffer, after which 50 l of 500 mM NaCl were added and the filters centrifuged for 15 min at 10,000 rpm. Trifluoroacetic acid was added to a final concentration of 1% and the peptides were desalted on C18 Oasis HLB extraction cartridges (Waters Corporation, Milford, MA, USA) and dried-down. 5.2. Isobaric labelling of peptides using tandem tass tags (TMT) The dried peptides were dissolved in triethylammonium bicarbonate buffer, and their concentration was determined using a Direct Detect IR spectrometer (Millipore). Equal amounts of each peptide sample were labeled with isobaric 10-plex Tandem Mass Tags (TMT, Thermo Scientific) according to the manufacturer’s instructions and mixed together. An internal control was prepared by pooling the nine Control samples and was used as a reference to express relative quantification values. Samples were desalted using C18 Oasis HLB extraction cartridges (Waters) and dried-down for later LC-MS/MS analysis. 5.3. Peptide identification Peptide samples were taken up in 0.1% formic acid and applied to an EASY-nLC 1000 nano- flow HPLC system (Thermo Fisher Scientific) coupled on-line with an orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). C18-based reverse phase separation was used with a 2-cm trap column and a 50-cm analytical column (EASY-Spray, Thermo Fisher Scientific). Peptides were loaded in buffer A (0.1% formic acid (v/v)) and eluted with a 300-min linear gradient of buffer B (90% ACN, 0.1% formic acid (v/v)) at 200 nL/min flow. Mass spectra were acquired in a data-dependent manner, with an automatic switch between MS and MS/MS, using a top-speed method and 30 s dynamic exclusion. MS spectra were acquired in the 400–1500 m/z range at 120,000 resolution, while HCD MS/MS were performed at 33 normalized collision energy and analyzed with 35,000 resolution in the orbitrap. S7 LC-MS/MS data were analyzed with Proteome Discoverer (version 2.1, Thermo Fisher Scientific) using SEQUEST-HT (Thermo Fisher Scientific) against a Uniprot database containing all sequences from Homo sapiens (February 2020; 148,000 entries). Database search parameters were selected as follows: trypsin digestion with two maximum missed cleavage sites, precursor mass tolerance of 800 ppm, fragment mass tolerance of 20 mmu. Met oxidation was considered a variable modification, while Cys carbamidomethylation and Lys and peptide N-terminal modification of +229.163 were set as fixed modifications for TMT. The corresponding inverted protein sequences were incorporated to the database for false discovery rate (FDR) calculation. Peptide identification from MS/MS data was performed using the probability ratio method [5], and the FDR of peptide identifications was calculated using the refined method [6,7], taking 1% FDR as a threshold for peptide identification. Peptides were assigned only to the best protein proposed by the Proteome Discoverer algorithm. References: 5. Martinez-Bartolome, S. et al. Properties of average score distributions of SEQUEST: the probability ratio method. Mol Cell Proteomics 7, 1135-1145 (2008). 6. Navarro, P. & Vazquez, J. A refined method to calculate false discovery rates for peptide identification using decoy databases. J Proteome Res 8, 1792-1796 (2009). 7. Bonzon-Kulichenko, E., Garcia-Marques, F., Trevisan-Herraz, M. & Vazquez, J. Revisiting peptide identification by high-accuracy mass spectrometry: problems associated with the use of narrow mass precursor windows. J Proteome Res 14, 700-710 (2015). 5.4. Quantification at the peptide and protein levels The quantitative information extracted from the MS/MS spectra by Proteome Discoverer was integrated from the spectrum level to the peptide level and then to the protein level on the basis of the WSPP model [8] and the systems biology triangle algorithm [9] using the SanXoT software package [10]. Briefly, the log2-ratio of every scan was calculated using the TMT reporter ion intensities coming from samples A and B. The log2-ratio of every peptide was then calculated as the weighted average of its scans, whereas the quantification of each protein was the weighted average of its peptides, and averaged of all the protein values [8]. Peptide quantification weights were calculated from the corresponding scan weights and the peptide variance, and protein quantification weights were calculated from the corresponding peptide weights and the protein variance. Thus, protein quantification was defined by a Zq S8 value as a normalized log2-ratio at the protein level expressed in standard deviation units [8]. Outliers at the peptide and protein levels were detected at 1% FDR, and a FDRq value < 0.05 was defined as significant [8] . References: 8. Navarro, P. et al. General statistical framework for quantitative proteomics by stable isotope labeling. J Proteome Res 13, 1234-1247 (2014). 9. Garcia-Marques, F. et al. A Novel Systems-Biology Algorithm for the Analysis of Coordinated Protein Responses Using Quantitative Proteomics. Mol Cell Proteomics 15, 1740-1760 (2016). 10. Trevisan-Herraz, M. et al. SanXoT: a modular and versatile package for the quantitative analysis of high-throughput proteomics experiments. Bioinformatics 35, 1594-1596 (2019). 6. Quantification of TTR peptides by mass spectrometry. Calibration experiments Calibration experiments were previously carried out to test the accuracy of the quantification using known concentrations of reference peptides bradykinin (765.85 Da), bombesin (1619.85 Da) and adrenocorticotropic hormone (ACTH, 2465.67 Da), in addition to angiotensin-II peptide (1045.54 Da). Peptide relative intensities were obtained from MS spectra in the same conditions as for peptide mass fingerprinting analysis by MALDI-TOF MS (see above). Reference peptides were quantified with respect to the angiotensin-II peptide as a ratio of the relative intensities of peptide/angiotensin-II. The reference peptide concentrations used were 2-100 fmol, with 4 fmol of angiotensin-II, and 4-400 fmol, with 40 fmol of angiotensin-II. To quantify transthyretin (TTR) peptides, the angiotensin-II peptide was added as an internal standard to obtain a relative intensity of the TTR peptides. The accuracy of MS peptide quantification was confirmed with calibration experiments carried out with increasing concentrations of reference peptides (molecular mass range from ~ 765 to 2465 Da) (Figure S4). The calibration experiments showed a strict correlation between peptide concentration and relative intensity for each peptide (Figure S4). TTR peptides with 1366.75, 1394.62, 1416.77, 1522.71, 2451.20 and 3140.51 Da were identified by MASCOT database search algorithm (Matrix Science, http://www.matrixscience.com), in addition to 833.40 Da TTR peptide, identified by FindPept tool (ExPASy, https://web.expasy.org/findpept/) (Figure 4A). Accordingly, TTR peptides from MALDI-TOF MS spectra were quantified in the mass rage of the calibration experiments (TTR peptides from 833.40 to 2451.20 Da) as relative intensities (ratio TTR peptide intensity/angiotensin-II intensity). S9 7. Detection of the allele distribution of haptoglobin α in HF and HDF patients Haptoglobin is a glycoprotein with  and  chains. In human, haptoglobin exists in two allelic forms designated Haptoglobin 1 (Hp1) and Haptoglobin 2 (Hp2) that results in three known phenotypes, Hp1-1, Hp2-2 and the heterozygous phenotype Hp2-1. Hp2 contain the allele Hp2 that is the product of a partial Hp1 gene duplication [11]. Thus, the product of Hp2 gives a protein with ~ 7 kDa greater than that of Hp1. In our experiments, haptoglobin 2 was detected as proteins 20 and 21 (Figure 3A), showing similar intensity values in both HF and HDF patient samples (Figure 3B). In these experiments, haptoglobin 1 was significantly detected with higher levels in HF patients than in HDF patients (Figure 1B, protein f; and Figure 3B). However, the allele distribution between HF and HDF groups should be tested. To these end, we analysed the expression of haptoglobin 2 and 1 in HF and HDF samples by western blot using a specific anti- haptoglobin  antibody. The results showed two proteins identified by the antibody, which according to the molecular mass standards used, would correspond to haptoglobin 2 and 1 (Figure S3). These results also showed that three HF patients (patients 3, 6, and 7) had not haptoglobin 2 expression –patient HF 9 showed a very slight expression of haptoglobin 2– (Figure S3), having therefore the phenotype Hp1-1. On the other hand, only one HDF patient (patient 6) had not haptoglobin 2, being of the Hp1-1 phenotype. As expected, the phenotype Hp1-1 induces more expression of haptoglobin 1 than the phenotypes Hp2-1 and Hp2-2 (Figure S3, see patients HF 3, 6 and 7 and HDF 6), and therefore, the presence of more subjects with phenotype Hp1-1 among HF patients can be the cause of the higher levels of haptoglobin 1 found in this group. References: 11. Yang, F., Brune, J. L., Baldwin, W. D., Barnett, D. R. & Bowman, B. H. Identification and characterization of human haptoglobin cDNA. Proc Natl Acad Sci U S A 80, 5875-5879 (1983). S10 8. Supplementary Table S1 Table S1. Proteins identified by MALDI-TOF MS in plasma samples of HF and HDF patients. N° Protein Accession no. a Gene name Theoretica l mass (Da) Score b Peptides matched/ searched % Coverage Lift c (score) 1 Alpha-2-macroglobulin P01023 A2M 164613 95 24/86 20 1148.62(63) 2 Ceruloplasmin P00450 CP 122983 112 19/66 24 2155.05(80) 3 Inter-alpha-trypsin inhibitor heavy chain H4 Q14624 ITIH4 103521 48 6/26 10 2184.07(50) 7 Serotransferrin P02787 TF 79294 152 25/95 33 1881.91(91) 12 Alpha-1-antitrypsin P01009 SERPINA1 46737 98 10/41 27 1641.83(99) 13 Apolipoprotein A-IV P06727 APOA4 45371 127 17/72 37 1352.69(76) 14 Haptoglobin β P00738 HP 27265 109 14/85 33 1707.87(75) 15 Haptoglobin β P00738 HP 27265 123 13/29 33 18 Apolipoprotein A-I P02647 APOA1 30759 191 23/98 71 19 Retinol-binding protein 4 P02753 RBP4 23337 70 5/13 33 2693.10(65) 20 Haptoglobin α2 P00738 HP 15945 62 8/52 21 21 Haptoglobin α2 P00738 HP 15945 60 6/38 16 3432.62(70) 22 Transthyretin P02766 TTR 13761 64 6/65 61 2451.20(189) 23 Haptoglobin α1 P00738 HP 9192 64 10/81 19 1708.91(91) 24 Serum amyloid A1 P0DJI8 SAA1 13581 63 5/39 55 a, Accession number in UniProt database (https://www.uniprot.org). b,Protein identification scores > 56 were significant (p < 0.05) in the MASCOT database search algorithm. c, MALDI LIFT-TOF/TOF MS identification mode; the m/z of the fragmented parental peptide is indicated; MASCOT scores (in parenthesis) > 28 were significant (p < 0.05). Proteins marked in bold were significant by DIGE quantification (Figure 1; p < 0.05, t-test) with higher levels in plasma samples of HF compared with HDF patients. S11 9. Supplementary Table S2 Table S2. Proteins quantified in LC-MS/MS analysis in plasma samples of HDF compared with HF patients. Protein Accession no. a Gene name Th eo re tic al m as s (D a) Pe pt id es id en tif ie d Zq v al ue b Zq v al ue (c ol ou r c od e) c cDNA FLJ53691, highly similar to Serotransferrin B4E1B2 74832 4 3.04 *,# Trypsin-1 P07477 PRSS1 26558 2 2.42 * Immunoglobulin heavy constant alpha 1 P01876 IGHA1 37655 14 2.09 * Dystroglycan Q14118 DAG1 97441 2 1.77 Immunoglobulin heavy variable 4-28 A0A0C4DH34 IGHV4-28 13124 3 1.57 Immunoglobulin delta heavy chain P0DOX3 56224 2 1.50 Immunoglobulin heavy constant gamma 3 P01860 IGHG3 41287 6 1.43 Immunoglobulin heavy variable 3-7 P01780 IGHV3-7 12943 2 1.42 Rheumatoid factor G9 light chain (Fragment) A0N5G3 V-lambda-3 12897 2 1.32 IBM-A3 heavy chain variable region (Fragment) A0A0X9UWM4 14369 2 1.28 Uncharacterized protein DKFZp686K04218 (Fragment) Q7Z379 DKFZp686K04218 51620 2 1.23 Vitronectin D9ZGG2 VTN 54306 5 1.18 Immunoglobulin heavy constant gamma 4 P01861 IGHG4 35941 7 1.11 Ig heavy chain variable region (Fragment) A0A068LKQ8 12449 2 1.05 IBM-B3 light chain variable region (Fragment) A0A109PW74 11399 2 1.03 Immunoglobulin kappa constant P01834 IGKC 11765 8 1.01 V1-22 protein (Fragment) Q5NV88 V1-22 10643 2 1.00 Alpha-2-macroglobulin P01023 A2M 163291 70 0.94 Immunoglobulin heavy constant gamma 1 P01857 IGHG1 36106 17 0.93 Properdin P27918 CFP 51276 3 0.87 B cell receptor heavy chain variable region (Fragment) A0A1C9J6R2 14524 2 0.87 Immunoglobulin lambda-like polypeptide 5 B9A064 IGLL5 23063 4 0.87 Immunoglobulin lambda constant 2 P0DOY2 IGLC2 11294 2 0.85 Cartilage oligomeric matrix protein P49747 COMP 82860 4 0.79 Immunoglobulin lambda constant 7 A0M8Q6 IGLC7 11254 4 0.72 Immunoglobulin heavy variable 3-49 A0A0A0MS15 IGHV3-49 13056 3 0.69 Immunoglobulin heavy constant alpha 2 P01877 IGHA2 36591 2 0.67 Serum amyloid A protein D3DQX7 SAA1 13562 2 0.65 Carboxypeptidase N subunit 2 P22792 CPN2 60557 9 0.62 Immunoglobulin lambda-like polypeptide 1 P15814 IGLL1 22963 4 0.61 MS-F1 light chain variable region (Fragment) A0A0X9V9B3 12108 2 0.60 Immunoglobulin kappa variable 3D-20 A0A0C4DH25 IGKV3D-20 12515 2 0.59 Beta-Ala-His dipeptidase Q96KN2 CNDP1 56706 7 0.56 Immunoglobulin alpha-2 heavy chain P0DOX2 48934 4 0.54 MS-F1 heavy chain variable region (Fragment) A0A125U0V1 13509 2 0.51 Immunoglobulin kappa variable 4-1 P06312 IGKV4-1 13380 2 0.51 Ig heavy chain variable region (Fragment) A0A2U8J8Y4 IgH 12010 2 0.46 Pregnancy zone protein P20742 PZP 163863 16 0.44 IBM-A1 light chain variable region (Fragment) A0A0X9USN3 11626 2 0.44 Myosin-reactive immunoglobulin light chain variable region (Fragment) Q9UL83 11834 2 0.42 Immunoglobulin kappa variable 3D-7 A0A0C4DH55 IGKV3D-7 13148 2 0.42 IBM-B2 heavy chain variable region (Fragment) A0A125QYY9 13569 2 0.39 Fibulin-1 P23142 FBLN1 77214 9 0.38 Afamin P43652 AFM 69069 17 0.38 Carboxypeptidase N catalytic chain P15169 CPN1 52286 4 0.37 Biotinidase P43251 BTD 61133 4 0.36 IgGFc-binding protein Q9Y6R7 FCGBP 572017 2 0.33 Vitamin K-dependent protein S P07225 PROS1 75123 18 0.27 Thyroxine-binding globulin P05543 SERPINA7 46325 14 0.26 Immunoglobulin kappa variable 3-7 (non-functional) (Fragment) A0A075B6H7 IGKV3-7 12783 2 0.24 S12 Ig heavy chain variable region (Fragment) A0A2U8J8Z6 IgH 10905 2 0.22 Fibronectin P02751 FN1 272320 54 0.22 Apolipoprotein(a) P08519 LPA 501319 10 0.21 Complement factor B P00751 CFB 85533 18 0.21 Immunoglobulin kappa variable 1-6 A0A0C4DH72 IGKV1-6 12697 2 0.19 Coagulation factor IX P00740 F9 51778 5 0.18 CP protein A5PL27 CP 122205 33 0.15 Carbonic anhydrase 1 (Fragment) E5RFE7 CA1 21402 3 0.13 Apolipoprotein C-I P02654 APOC1 9332 5 0.12 Hyaluronan-binding protein 2 Q14520 HABP2 62672 10 0.12 Immunoglobulin kappa variable 3D-15 A0A087WSY6 IGKV3D-15 12534 3 0.11 von Willebrand factor P04275 VWF 309265 16 0.11 Gelsolin A0A0A0MS51 GSN 82526 14 0.11 Immunoglobulin J chain P01591 JCHAIN 18099 4 0.09 Hepatocyte growth factor activator Q04756 HGFAC 70682 7 0.09 Complement C3 P01024 C3 187148 100 0.09 SPARC-like protein 1 Q14515 SPARCL1 75208 2 0.09 Complement component C8 gamma chain P07360 C8G 22277 7 0.07 IGK@ protein Q6PIL8 IGK@ 25834 2 0.05 Selenoprotein P P49908 SELENOP 43174 2 0.05 Prothrombin P00734 F2 70037 31 0.05 Apolipoprotein F Q13790 APOF 35399 3 0.05 cDNA FLJ51266, highly similar to Vitronectin B7Z553 23605 3 0.05 Angiotensinogen (Fragment) A7L3A3 AGT 9407 4 0.04 N-acetylmuramoyl-L-alanine amidase Q96PD5 PGLYRP2 62217 8 0.02 Immunoglobulin heavy variable 3-9 P01782 IGHV3-9 12945 3 0.01 Immunoglobulin heavy constant delta P01880 IGHD 42353 4 0.00 Immunoglobulin heavy variable 3-15 A0A0B4J1V0 IGHV3-15 12926 2 -0.01 Cystatin-C P01034 CST3 15799 6 -0.02 Immunoglobulin lambda variable 3-10 A0A075B6K4 IGLV3-10 12441 2 -0.02 Myosin-reactive immunoglobulin kappa chain variable region (Fragment) Q9UL86 11928 3 -0.05 MS-C1 heavy chain variable region (Fragment) A0A125U0U7 13097 2 -0.05 Immunoglobulin heavy constant gamma 2 P01859 IGHG2 35901 13 -0.06 Transforming growth factor-beta-induced protein ig-h3 Q15582 TGFBI 74681 3 -0.06 Angiotensinogen (Serpin peptidase inhibitor, clade A, member 8) B0ZBE2 AGT 53154 5 -0.07 EGF containing fibulin-like extracellular matrix protein 1 isoform 2 A0A0S2Z3V1 EFEMP1 45723 5 -0.07 Coagulation factor X P00742 F10 54732 5 -0.07 C4b-binding protein alpha chain P04003 C4BPA 67033 26 -0.08 Peroxiredoxin-2 P32119 PRDX2 21892 4 -0.09 Ig heavy chain variable region (Fragment) A0A2U8J9B3 IgH 12311 2 -0.10 Ig heavy chain variable region (Fragment) A0A2U8J8R6 IgH 10922 2 -0.14 Serum albumin P02768 ALB 69367 76 -0.16 Plasma kallikrein P03952 KLKB1 71370 12 -0.16 Pigment epithelium-derived factor P36955 SERPINF1 46312 11 -0.17 Sulfhydryl oxidase 1 O00391 QSOX1 82578 2 -0.18 Apolipoprotein C-IV P55056 APOC4 14553 2 -0.18 Ig heavy chain variable region (Fragment) A0A2U8J8Q6 IgH 10379 2 -0.19 Fibrinogen alpha chain P02671 FGA 94973 41 -0.20 Platelet glycoprotein Ib alpha chain P07359 GP1BA 71540 2 -0.20 Coagulation factor V P12259 F5 251703 12 -0.21 Chromogranin-A P10645 CHGA 50688 9 -0.22 Adiponectin A A0A024RAB9 C1QB 26722 5 -0.22 Immunoglobulin heavy variable 3-21 A0A0B4J1V1 IGHV3-21 12840 3 -0.23 Apolipoprotein D P05090 APOD 21276 7 -0.23 Fetuin-B Q9UGM5 FETUB 42055 5 -0.24 Immunoglobulin heavy variable 4-4 A0A075B6R2 IGHV4-4 12848 4 -0.25 Inter-alpha-trypsin inhibitor heavy chain H4 Q14624 ITIH4 103357 26 -0.26 Complement factor H P08603 CFH 139096 54 -0.26 Heavy chain of factor I (Fragment) Q6LAM1 35883 2 -0.26 Immunoglobulin heavy variable 3-72 A0A0B4J1Y9 IGHV3-72 13203 3 -0.28 Immunoglobulin kappa variable 3D-11 A0A0A0MRZ8 IGKV3D-11 12625 2 -0.28 C-reactive protein P02741 CRP 25039 6 -0.29 Monocyte differentiation antigen CD14 P08571 CD14 40076 4 -0.29 Actin, cytoplasmic 1 P60709 ACTB 41737 5 -0.30 S13 Apolipoprotein B-100 P04114 APOB 515605 178 -0.31 Complement factor D P00746 CFD 27033 9 -0.31 Complement component C8 alpha chain P07357 C8A 65163 15 -0.31 Complement component C6 P13671 C6 104786 17 -0.31 Lipopolysaccharide-binding protein P18428 LBP 53384 5 -0.33 Glutathione peroxidase 3 P22352 GPX3 25552 5 -0.33 Fibrinogen beta chain P02675 FGB 55928 43 -0.34 Complement factor I P05156 CFI 65750 17 -0.34 Inter-alpha-trypsin inhibitor heavy chain H1 P19827 ITIH1 101389 18 -0.34 Apolipoprotein A-I P02647 APOA1 30778 38 -0.35 Inter-alpha-trypsin inhibitor heavy chain H2 P19823 ITIH2 106463 21 -0.37 Alpha-1B-glycoprotein P04217 A1BG 54254 10 -0.37 Immunoglobulin kappa light chain P0DOX7 23379 4 -0.38 Complement component C8 beta chain P07358 C8B 67047 13 -0.38 Serotransferrin P02787 TF 77064 56 -0.38 Complement C5 P01031 C5 188305 38 -0.39 Fibrinogen gamma chain P02679 FGG 51512 31 -0.39 GCT-A1 light chain variable region (Fragment) A0A0X9TDD0 2 SV 12436 2 -0.39 Alpha-2-HS-glycoprotein P02765 AHSG 39341 10 -0.41 Cystatin-M Q15828 CST6 16511 2 -0.42 Platelet basic protein P02775 PPBP 13894 2 -0.43 Fibronectin 1, isoform CRA_n A0A024R462 FN1 259211 2 -0.43 Immunoglobulin heavy variable 3-74 A0A0B4J1X5 IGHV3-74 12840 4 -0.43 Plasma serine protease inhibitor P05154 SERPINA5 45675 5 -0.44 Vasorin Q6EMK4 VASN 71713 2 -0.46 Mannan-binding lectin serine protease 1 P48740 MASP1 79247 5 -0.47 Carbonic anhydrase 1 (Fragment) E5RFL2 CA1 13256 2 -0.48 Lectin galactoside-binding soluble 3 binding protein isoform 1 A0A0S2Z3Y1 LGALS3BP 65331 7 -0.49 Heparin cofactor 2 P05546 SERPIND1 57071 15 -0.49 Kininogen-1 P01042 KNG1 71957 28 -0.52 Prenylcysteine oxidase 1 Q9UHG3 PCYOX1 56640 3 -0.54 Basement membrane-specific heparan sulfate proteoglycan core protein P98160 HSPG2 468830 2 -0.57 Mutant hemoglobin alpha 2 globin chain A0A0K2BMD8 HBA2 15258 6 -0.60 Adiponectin B A0A024RAA7 C1QC 25774 2 -0.60 IBM-B1 light chain variable region (Fragment) A0A125QYY8 12167 2 -0.63 Complement factor H-related protein 1 Q03591 CFHR1 37651 3 -0.63 Inter-alpha-trypsin inhibitor heavy chain H3 Q06033 ITIH3 99849 15 -0.63 Clusterin P10909 CLU 52495 18 -0.65 Complement C1s subcomponent P09871 C1S 76684 16 -0.66 Coagulation factor XIII B chain P05160 F13B 75511 15 -0.67 Vitamin K-dependent protein C P04070 PROC 52071 3 -0.69 N90-VRC38.01 light chain variable region (Fragment) A0A1W6IYJ9 12352 4 -0.69 Plasminogen P00747 PLG 90569 32 -0.69 Serum amyloid P-component P02743 APCS 25387 7 -0.69 C4b-binding protein beta chain P20851 C4BPB 28357 6 -0.71 Alpha-1-antitrypsin P01009 SERPINA1 46737 38 -0.71 Testicular tissue protein Li 61 A0A140VJI7 60674 7 -0.72 Epididymis secretory sperm binding protein A0A384N669 38429 10 -0.72 Carboxypeptidase B2 Q96IY4 CPB2 48424 7 -0.72 Coagulation factor XIII A chain P00488 F13A1 83267 4 -0.74 Kallistatin P29622 SERPINA4 48542 12 -0.74 Insulin-like growth factor II P01344 IGF2 20140 2 -0.76 Alpha-2-antiplasmin P08697 SERPINF2 54566 12 -0.77 Corticosteroid-binding globulin P08185 SERPINA6 45141 7 -0.78 Immunoglobulin kappa variable 1-27 A0A075B6S5 IGKV1-27 12712 2 -0.79 Phosphatidylinositol-glycan-specific phospholipase D P80108 GPLD1 92336 7 -0.82 Vitamin D-binding protein P02774 GC 52918 29 -0.84 Coagulation factor XII P00748 F12 67792 8 -0.84 Complement C2 P06681 C2 83268 16 -0.85 Hemoglobin subunit delta P02042 HBD 16055 9 -0.86 Apolipoprotein L1 O14791 APOL1 43974 7 -0.86 Phospholipid transfer protein P55058 PLTP 54739 3 -0.86 Hemoglobin subunit beta P68871 HBB 15998 8 -0.86 Vitamin D binding protein (Fragment) A0A1B1CYC5 Gc 3718 2 -0.86 Insulin-like growth factor binding protein 4, isoform A0A024R1U8 IGFBP4 27934 2 -0.86 S14 CRA_a Serpin peptidase inhibitor clade G member 1 isoform 4 A0A0S2Z333 SERPING1 19647 10 -0.87 Serum paraoxonase/arylesterase 1 P27169 PON1 39731 7 -0.88 HCG40889, isoform CRA_b A0A024R962 hCG_40889 139070 2 -0.88 Mannan-binding lectin serine protease 2 O00187 MASP2 75702 2 -0.88 Protein AMBP P02760 AMBP 38999 18 -0.90 Immunoglobulin lambda variable 2-8 P01709 IGLV2-8 12382 2 -0.95 Histidine-rich glycoprotein P04196 HRG 59578 12 -0.96 Retinol-binding protein 4 P02753 RBP4 23010 8 -0.96 Apolipoprotein C-II isoform 1 A0A024R0T9 APOC4-APOC2 11284 4 -0.97 Complement C1r subcomponent P00736 C1R 80119 12 -0.98 Serum albumin Q56G89 69084 2 -0.99 Apolipoprotein M O95445 APOM 21253 4 -1.01 Complement component C7 P10643 C7 93518 17 -1.04 Serpin peptidase inhibitor, clade A (Alpha-1 antiproteinase, antitrypsin) member 3, isoform CRA_c A0A024R6P0 SERPINA3 47651 22 -1.12 Hepatocyte growth factor-like protein P26927 MST1 80320 5 -1.12 Attractin O75882 ATRN 158537 10 -1.13 Serpin peptidase inhibitor clade G member 1 A0A348GSH7 SERPING1 49757 8 -1.15 Serum amyloid A-1 protein P0DJI8 SAA1 13532 7 -1.17 Apolipoprotein A-II P02652 APOA2 11175 7 -1.17 Complement C1r subcomponent-like protein Q9NZP8 C1RL 53498 2 -1.20 Protein Z-dependent protease inhibitor Q9UK55 SERPINA10 50707 2 -1.21 Alpha-1-acid glycoprotein 2 P19652 ORM2 23603 9 -1.22 Antithrombin-III P01008 SERPINC1 52602 29 -1.22 Prostaglandin-H2 D-isomerase P41222 PTGDS 21029 5 -1.24 Beta-2-microglobulin P61769 B2M 13715 4 -1.27 Complement C4-A P0C0L4 C4A 192785 65 -1.30 Complement factor H-related protein 2 P36980 CFHR2 30651 4 -1.36 Protein S100-A9 P06702 S100A9 13242 6 -1.38 Complement C4-B P0C0L5 C4B 192751 4 -1.42 Insulin-like growth factor-binding protein 3 P17936 IGFBP3 31674 4 -1.44 Complement component C9 P02748 C9 63173 16 -1.46 Hemopexin P02790 HPX 51676 24 -1.47 Immunoglobulin kappa variable 3-20 P01619 IGKV3-20 12557 2 -1.51 CD44 antigen P16070 CD44 81538 2 -1.56 Immunoglobulin heavy variable 5-51 A0A0C4DH38 IGHV5-51 12675 4 -1.58 Alpha-1-acid glycoprotein V9HWF6 HEL-S-153w 23512 6 -1.61 Serum amyloid A-4 protein P35542 SAA4 14747 4 -1.62 Insulin-like growth factor-binding protein complex acid labile subunit P35858 IGFALS 66035 10 -1.63 Insulin-like growth factor-binding protein 2 P18065 IGFBP2 34814 4 -1.63 CD5 antigen-like O43866 CD5L 38088 11 -1.65 Haptoglobin P00738 HP 45205 30 -1.71 Ficolin-3 O75636 FCN3 32903 5 -1.74 Apolipoprotein A-IV P06727 APOA4 45372 33 -1.81 Apolipoprotein H (Beta-2-glycoprotein I), isoform CRA_a A0A384NKM6 APOH 38298 12 -1.82 Leucine-rich alpha-2-glycoprotein P02750 LRG1 38178 5 -1.86 Immunoglobulin heavy constant mu P01871 IGHM 49440 15 -1.91 Transthyretin P02766 TTR 15887 7 -1.97 Uncharacterized protein DKFZp686G11190 Q6MZQ6 DKFZp686G11190 52043 3 -2.06 * Apolipoprotein E P02649 APOE 36154 17 -2.15 * Full-length cDNA clone CS0DD006YL02 Q86TT1 41273 2 -2.30 * Testicular tissue protein Li 227 A0A140VK00 34259 17 -2.30 * Apolipoprotein C-III A3KPE2 APOC3 10852 5 -2.48 * Haptoglobin-related protein P00739 HPR 39030 7 -3.19 *,# Epididymis secretory sperm binding protein A0A384MDQ7 46723 2 -4.45 *,# a, Accession number in UniProt database (https://www.uniprot.org). b, Protein quantification values (Zq) are normalized log2-ratios expressed in standard deviation units: Zq > 0 and Zq < 0 indicate increased or decreased, respectively, protein abundance in HDF compared with HF patients. c, Zq values in a colour scale; red and blue represent the increased or decreased protein levels, respectively, in HDF compared with HF. * Zq  2, and  -2, were significant (p  0.05); #, significant FDRq value (< 0.05). S15 10. Supplementary Figure S1 Figure S1. Plasma samples from HF and HDF patients were depleted from albumin and IgG. Plasma samples from patients on high-flux haemodialysis (HF) or on-line haemodiafiltration (HDF) were pooled, albumin and IgG depleted and ran into sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and the gel was stained with Coomassie blue for protein detection. The numbers on the left indicate the apparent molecular mass in kDa. The figure shows plasma samples from HF and HDF patients before and after albumin and IgG depletion (-Alb/IgG) in a whole stained gel. Plasma -Alb/IgG HF HDF HF HDF 97 - 66 - 30 - 20 - 14 - 45 - kDa S16 11. Supplementary Figure S2 Figure S2. Differential protein detection of individual plasma samples of HF combined with HDF patients by fluorescence in gel electrophoresis (DIGE). The images show the combinations of individual paired and Cy-labelled samples of nine HF and HDF patients performed in the same way as described in Figure 1A. The scanned gel displays fluorescence-labelled proteins; proteins in the HF or HDF samples were visualised in red (Cy5-labelled) or green (Cy3-labelled). Proteins present in both HF and HDF samples were visualised in yellow due to the merge of the red and green labels (HF+HDF). The numbers on the left indicate the apparent molecular mass. The differentially detected proteins quantified in Figure 1B, a to c, are indicated in by arrows in the highest labelling colour code (red or green, HF patients). The fluorescence image corresponds to whole gels and adjacent empty lanes are not shown. HF+HDF 5 6 8 b c a HF 5 6 8 HDF 5 6 8 97 - 66 - 30 - 20 - 14 - 45 - kDa 97 - 66 - 30 - 20 - 14 - 45 - kDa HF+HDF 2 3 4 7 c a HF+HDF 9 10+1 HDF 2 3 4 7 HF 9 10 HF 2 3 4 7 HDF 9 1 b c a b S17 12. Supplementary Figure S3 HF HDF HF HDF HF HDF HF HDF 35 kDa- 15 kDa- 25 kDa- Haptoglobin 2 Haptoglobin 1 35 kDa- 15 kDa- 25 kDa- HF HDF HF HDF HF HDF HF HDF 35 kDa- 15 kDa- 25 kDa- HF HDF HF Haptoglobin 2 Haptoglobin 1 Haptoglobin 2 Haptoglobin 1 9 9 10 5 5 6 6 7 7 8 8 1 1 2 2 3 3 4 4 A S18 HF HDF HF HDF HF HDF HF HDF HF HDF HF HDF HF HDF HF HDF HF HDF HF 9 9 10 5 5 6 6 7 7 8 8 1 1 2 2 3 3 4 4 B 95 KDa- 55 KDa- 95 KDa- 55 KDa- 95 KDa- 55 KDa- Figure S3. Haptoglobin  detection by western blot in HF and HDF plasma samples. (A) Individual albumin- and IgG-depleted plasma samples (5 μg of protein) from 10 and 9 HF and HDF patients, respectively, were analysed independently by western blotting as describe in Methods using a mouse monoclonal anti-haptoglobin  antibody (sc-376893, Santa Cruz Biotechnology). In the images, arrows show the haptoglobin 2 and 1 detected. The numbers on the left indicate the apparent molecular mass from standards. (B) Blotted proteins were staining with Fast Green as loading control of the analyzed samples. . The upper piece of the PVDF membranes containing most proteins was used for staining. Images show whole blots. S19 11. Supplementary Figure S4 0 100 200 300 400 0 5 10 15 20 25 Bradykinin Bombesin ACTH peptide concentration (fmol) R at io p ep tid e/ An gI I 0 20 40 60 80 100 0 5 10 15 20 25 Bradykinin Bombesin ACTH peptide concentration (fmol) R at io p ep tid e/ An gI I R at io p ep tid e/ An gI I R at io p ep tid e/ An gI I Figure S4. Quantification of the peptide intensities in the mass spectra from MALDI-TOF MS. Calibration experiments showing the linear correlation between peptide concentration and the ratio peptide intensity/angiotensin-II peptide intensity as relative intensity. Peptides used were bradykinin (765.85 Da), bombesin (1619.85 Da), and adrenocorticotropic hormone (ACTH, 2465.67 Da). Peptide intensities were obtained from mass spectra by MALDI-TOF MS analysis, in the same conditions as mass fingerprinting analysis and using angiotensin-II peptide (1045.53 Da) as internal standard. Peptide concentrations used were 2-100 fmol, and 4 fmol of angiotensin-II (left graph), or 4-400 fmol, with 40 fmol of angiotensin-II (right graph). The correlation between peptide concentration and relative intensity for each peptide was very significant, p < 0.0005 (Pearson test). S20 13. Supplementary Figure S5 HF HDF 5 6 7 8 5 6 7 8 95 KDa- 55 KDa- HF HDF 9 10 9 95 KDa- 55 KDa- HF HDF 95 KDa- 55 KDa- 1 2 3 4 1 2 3 4 Figure S5. Loading control in western blot experiments for anti-TTR antibody detection. Blotted proteins were staining with Fast Green in the PVDF membranes as loading control of analyzed samples from HF and HDF patients. The upper piece of the membranes containing most proteins was used for staining and normalization dividing the TTR level of the western blot by the total level of stained proteins of the same loading lane. Images show stained whole blots. S21 15. Supplementary Figure S6 Figure S6. Correlation between the molecular mass ( 40 kDa) and the quantification value (Zq) in the proteins identified in plasma samples of HDF compared with HF patients by LC-MS/MS analysis. Protein quantification values (Zq) are the normalized log2-ratios expressed in standard deviation units: Zq > 0 and Zq < 0 indicate increased or decreased, respectively, protein abundance in HDF compared with HF patients. Zq  2, and  -2, were significant (p  0.05). Red and blue lines show the values Zq=2 and Zq= -2, respectively. The continuous black line shows de linear regression and correlation was significant (p < 0.0043, Pearson test). 50 00 10 00 0 15 00 0 20 00 0 25 00 0 30 00 0 35 00 0 40 00 0 -4 -3 -2 -1 0 1 2 3 protein abundance HDF > HF HDF < HF Theoretical molecular mass (Da) Zq va lu e (H D F vs . H F) Zq va lu e (H D F vs . H F) 012 56 .5 82 10 57 .3 64 10 58 .3 72 21 55 .0 58 23 48 .1 06 10 60 .3 82 12 11 .6 40 91 7. 33 3 21 84 .0 74 12 50 .6 13 12 03 .7 38 16 41 .7 57 16 71 .8 29 20 45 .0 17 15 19 .7 44 21 72 .0 64 23 34 .0 85 24 15 .1 57 20 56 .0 67 16 67 .7 29 19 05 .8 04 25 64 .2 75 21 67 .0 44 22 93 .0 02 22 59 .9 82 27 01 .2 10 3 0:F7 MS Raw 0 1 2 3 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0.00 21 55 .0 51 15 19 .7 14 22 92 .9 96 21 21 .9 35 20 73 .8 95 19 03 .7 87 87 9. 37 5 18 37 .8 86 13 71 .7 42 88 1. 30 1 11 48 .6 03 88 7. 36 7 15 75 .8 25 10 59 .3 65 16 47 .8 09 88 9. 36 8 25 19 .2 36 89 5. 37 5 99 3. 45 1 84 8. 30 1 20 88 .9 27 10 17 .4 48 85 9. 34 5 83 0. 35 4 10 57 .3 74 94 0. 41 9 98 9. 48 5 11 31 .5 70 12 89 .6 06 12 20 .4 58 12 59 .5 37 21 71 .0 27 20 45 .0 21 12 03 .5 36 16 13 .7 35 15 89 .8 38 18 48 .8 31 14 31 .6 15 26 85 .2 34 16 36 .7 80 16 72 .8 08 21 77 .0 20 25 31 .2 06 20 61 .0 08 26 32 .2 19 18 70 .8 33 24 55 .0 34 21 41 .8 64 20 90 .9 54 21 40 .0 39 22 59 .9 56 21 11 .0 10 25 05 .0 93 20 93 .9 34 24 30 .0 53 25 63 .1 95 24 71 .0 40 28 04 .3 88 25 53 .1 83 34 16 .6 42 2 0:E8 MS Raw 0 1 2 3 4 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 22 59 .9 51 11 48 .5 99 20 73 .8 83 18 70 .8 19 20 45 .0 10 88 6. 43 3 82 8. 43 4 12 55 .6 14 84 8. 27 7 12 59 .5 40 18 48 .8 11 11 31 .5 68 10 06 .4 48 20 87 .8 98 86 1. 04 1 12 10 .5 98 16 94 .7 95 11 70 .5 70 87 9. 37 2 14 18 .5 64 88 8. 34 6 88 9. 35 9 10 68 .5 27 20 57 .8 28 12 72 .6 38 10 40 .5 40 15 45 .7 47 84 2. 38 9 15 13 .7 37 16 72 .7 92 10 58 .3 70 11 06 .5 66 94 7. 48 9 85 0. 37 3 20 31 .8 16 89 5. 36 2 11 42 .5 84 16 26 .7 85 19 35 .8 24 18 86 .7 89 20 67 .0 00 16 58 .7 75 19 21 .8 28 20 60 .9 94 11 25 .5 70 22 73 .9 54 25 17 .0 74 19 13 .8 31 15 87 .7 78 18 53 .7 94 11 92 .5 29 12 77 .5 86 20 48 .9 94 21 85 .9 151 84 0. 86 3 24 20 .1 99 18 99 .8 22 20 89 .8 62 16 20 .7 83 25 84 .0 19 18 31 .7 82 20 95 .8 62 20 08 .3 11 16 36 .7 75 14 54 .7 13 15 33 .7 04 16 04 .7 66 16 42 .7 68 18 92 .7 86 20 25 .8 72 22 81 .9 14 19 05 .9 79 21 09 .9 90 19 97 .0 04 21 59 .9 28 21 63 .0 61 25 62 .0 42 24 97 .1 33 33 38 .4 30 33 70 .4 03 33 52 .4 17 31 99 .4 87 33 60 .3 78 33 75 .3 89 1 0:E7 MS Raw 0.00 0.25 0.50 0.75 1.00 1.25 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 1. Alpha-2-macroglobulin 2. Ceruloplasmin 3. Inter-alpha-trypsin inhibitor heavy chain H4 0.0 13 52 .6 89 97 6. 54 2 11 04 .5 94 84 8. 28 9 11 92 .6 03 98 0. 51 8 11 60 .6 17 84 2. 28 0 13 11 .7 30 10 12 .5 06 98 3. 56 7 95 9. 51 6 12 15 .6 70 14 39 .8 26 17 52 .9 82 86 4. 27 5 94 5. 52 6 15 85 .9 01 15 08 .7 82 11 32 .6 44 88 7. 39 3 12 58 .6 86 87 9. 38 4 11 64 .5 70 99 7. 49 3 10 83 .5 43 92 3. 53 1 13 74 .6 67 94 2. 46 4 83 6. 29 7 10 58 .3 91 12 20 .5 18 17 21 .8 52 10 57 .3 84 14 95 .7 03 13 38 .6 448 44 .2 87 12 57 .6 76 88 9. 38 8 11 25 .5 79 92 0. 45 1 88 1. 34 4 89 6. 42 0 16 56 .8 50 14 78 .7 56 10 49 .3 99 13 41 .6 69 99 3. 46 5 13 00 .6 98 20 84 .0 74 19 27 .9 78 12 83 .5 84 12 96 .6 48 11 76 .6 02 14 43 .7 85 18 27 .9 20 12 42 .4 98 17 42 .8 30 18 34 .9 34 15 65 .8 03 14 61 .7 97 18 05 .9 30 16 07 .8 79 17 73 .9 93 25 17 .1 15 18 49 .9 19 10 0:G8 MS Raw 0.0 0.5 1.0 1.5 2.0 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0.0 16 41 .8 28 20 90 .0 49 10 37 .5 82 10 78 .5 06 11 63 .4 40 11 32 .5 59 12 75 .6 58 99 3. 44 0 10 15 .5 86 10 57 .3 50 11 10 .5 55 13 55 .7 09 12 20 .4 67 14 03 .6 73 16 63 .7 96 10 19 .3 43 91 0. 46 6 10 47 .3 72 10 49 .3 65 13 33 .7 10 10 59 .3 85 99 7. 38 1 99 1. 37 0 13 07 .6 43 12 16 .6 15 11 66 .4 24 12 69 .5 60 21 12 .0 19 18 77 .9 08 22 07 .9 73 18 01 .7 06 18 56 .8 85 18 25 .8 76 18 93 .8 75 21 85 .9 94 27 57 .4 33 31 80 .3 58 27 31 .2 28 25 96 .2 46 27 15 .1 91 31 48 .3 65 16 0:K8 MS Raw 0.0 0.5 1.0 1.5 2.0 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0 12 83 .5 46 11 95 .5 21 10 29 .5 63 14 78 .7 11 16 00 .7 64 90 9. 40 5 15 86 .7 53 12 49 .6 07 16 46 .6 86 94 9. 42 1 15 61 .7 05 17 73 .9 94 12 63 .6 18 86 4. 46 1 10 61 .5 47 11 48 .5 49 10 12 .5 31 15 31 .6 64 15 65 .7 72 12 09 .5 26 90 0. 51 2 11 89 .5 51 16 60 .7 23 19 52 .9 31 18 81 .9 08 12 55 .6 04 13 23 .6 26 14 48 .6 32 14 18 .5 73 11 25 .5 31 14 91 .7 17 14 94 .6 82 13 50 .6 49 13 52 .6 61 15 79 .7 80 15 45 .6 82 21 71 .0 72 18 95 .9 52 12 13 .5 95 15 14 .6 45 13 38 .6 42 18 83 .8 67 99 7. 45 4 16 20 .7 91 12 76 .6 02 96 4. 50 6 14 64 .7 22 19 66 .9 35 83 3. 48 2 10 36 .4 98 15 91 .7 92 17 30 .8 47 12 20 .5 51 25 63 .3 05 13 17 .5 66 10 00 .4 74 11 62 .5 37 1 53 9. 68 4 15 52 .7 35 25 49 .2 95 12 11 .5 44 13 98 .5 7389 6. 44 0 15 21 .7 00 19 63 .8 93 16 16 .7 48 16 73 .7 76 13 86 .7 77 17 03 .8 28 83 6. 39 8 18 11 .9 18 17 59 .8 67 22 03 .0 70 20 01 .9 02 10 33 .5 50 10 17 .5 20 13 72 .6 59 22 32 .2 35 18 51 .9 09 22 12 .0 23 16 25 .7 72 14 42 .4 14 21 54 .9 44 20 70 .9 59 21 85 .0 89 20 84 .9 66 19 39 .8 75 19 25 .8 40 21 10 .0 53 22 65 .0 85 20 81 .0 17 22 25 .0 77 22 46 .2 48 23 77 .1 33 5 0:F8 MS Raw 0.0 0.2 0.4 0.6 0.8 1.0 1.2 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 7. Serotransferrin 12. Alpha-1-antitrypsin 13. Apolipoprotein A-IV_ 013 01 .6 48 12 83 .5 74 17 97 .9 00 10 12 .5 78 13 80 .7 03 12 26 .5 39 16 32 .7 89 86 9. 39 0 22 34 .1 26 14 11 .6 72 17 23 .9 39 12 52 .6 17 18 15 .8 66 88 7. 36 3 84 2. 26 2 10 57 .3 57 18 11 .9 15 87 3. 44 6 11 57 .6 16 88 9. 36 7 17 05 .8 91 13 86 .6 93 12 66 .5 55 87 9. 37 2 10 59 .3 70 83 1. 41 8 14 05 .6 68 85 9. 33 2 21 08 .9 82 12 30 .6 80 99 3. 56 1 13 15 .5 57 11 58 .5 95 13 18 .6 22 15 85 .8 03 24 39 .2 31 18 84 .0 08 20 37 .9 13 85 5. 51 0 86 1. 36 0 17 75 .8 49 12 19 .6 57 19 46 .0 11 17 43 .8 55 14 67 .7 81 21 02 .1 33 97 9. 55 3 12 35 .6 41 14 08 .6 81 15 80 .8 06 18 19 .9 02 14 62 .8 25 16 61 .9 15 12 68 .6 16 10 07 .5 78 16 75 .9 29 16 45 .7 98 23 09 .0 44 19 68 .0 05 16 89 .8 77 24 66 .2 51 18 70 .9 91 10 47 .4 92 14 95 .6 73 12 15 .6 29 24 53 .2 42 16 53 .7 63 21 37 .1 18 13 23 .6 06 17 65 .8 48 14 00 .6 51 16 12 .7 82 18 78 .9 98 14 84 .8 20 17 33 .9 01 16 02 .7 80 24 81 .2 56 26 77 .3 04 24 23 .2 27 24 94 .2 63 24 50 .2 50 21 41 .0 34 20 84 .8 87 20 94 .9 42 23 23 .0 75 20 51 .9 33 22 02 .1 24 20 65 .0 39 23 80 .2 00 21 69 .1 22 23 52 .1 87 24 09 .2 12 26 24 .3 35 22 06 .1 03 23 03 .1 68 11b 0:F10 MS Raw 0.0 0.5 1.0 1.5 2.0 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0.00 98 0. 49 6 17 52 .9 46 11 60 .5 89 17 21 .8 26 17 07 .8 11 17 94 .9 93 18 34 .9 14 14 95 .6 76 92 3. 51 9 92 0. 44 5 11 92 .5 75 11 64 .5 81 17 42 .7 97 10 12 .4 79 12 34 .6 90 1 72 3. 77 9 18 50 .9 05 17 37 .8 15 16 85 .7 51 12 03 .6 21 12 74 .6 24 14 18 .8 00 18 16 .9 74 15 09 .6 84 13 45 .6 30 17 74 .9 20 21 72 .0 411 84 5. 01 9 18 74 .8 67 12 0:J10 MS Raw 0 1 2 3 4 5 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0.0 98 0. 53 4 11 92 .6 20 11 60 .6 31 94 5. 54 7 92 3. 54 9 94 2. 48 7 17 07 .8 69 88 0. 49 9 17 21 .8 85 92 0. 47 7 84 2. 28 5 84 8. 30 5 10 57 .4 07 10 58 .4 07 17 53 .0 07 88 7. 39 2 85 8. 49 4 12 25 .6 65 10 02 .5 14 18 34 .9 73 13 12 .7 54 95 9. 54 8 14 40 .8 54 88 8. 39 5 88 9. 42 6 10 12 .5 19 86 0. 35 9 96 1. 51 4 13 67 .6 81 12 96 .6 68 11 64 .6 12 10 60 .4 19 95 8. 45 8 82 8. 35 2 83 1. 37 8 12 03 .6 54 12 34 .7 39 89 6. 45 1 84 4. 30 9 17 42 .8 56 12 74 .6 68 83 6. 30 8 20 95 .9 86 86 4. 28 4 92 6. 51 1 99 4. 52 8 10 18 .4 9082 9. 36 9 11 76 .6 22 89 9. 39 3 97 6. 53 5 10 66 .5 70 97 4. 51 5 14 18 .8 46 10 69 .4 09 12 90 .7 39 13 52 .6 99 11 82 .6 03 11 27 .6 07 12 15 .6 44 12 41 .6 30 13 45 .6 65 11 68 .5 65 20 63 .9 99 13 28 .7 31 18 48 .9 76 21 94 .1 00 13 81 .6 90 14 95 .7 26 17 29 .8 52 16 27 .8 44 15 32 .8 28 17 45 .8 27 17 37 .8 57 16 59 .8 70 21 27 .9 86 16 73 .8 75 18 14 .9 54 21 72 .0 98 18 56 .9 52 22 09 .0 90 11 0:H7 MS Raw 0.00 0.25 0.50 0.75 1.00 1.25 1.50 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 14. Haptoglobin  15. Haptoglobin  18. Apolipoprotein A-I 0.0 26 93 .1 59 27 25 .1 45 20 64 .9 83 34 32 .6 40 23 78 .2 33 34 46 .6 57 17 08 .8 69 13 03 .6 28 10 58 .3 68 10 57 .3 66 27 07 .1 55 33 63 .5 91 33 49 .5 68 24 10 .2 20 12 30 .5 98 13 04 .5 69 89 5. 46 9 89 9. 36 9 10 60 .3 78 10 69 .3 63 26 77 .1 21 11 06 .5 85 89 7. 35 7 90 1. 37 0 99 3. 43 0 15 05 .7 26 90 3. 38 0 29 62 .3 67 11 63 .4 46 13 17 .6 23 11 61 .5 03 12 85 .6 26 11 98 .6 18 12 20 .4 96 16 75 .7 66 17 17 .2 90 29 76 .3 88 13 47 .5 37 14 39 .6 74 26 49 .1 36 20 78 .9 96 17 23 .8 00 34 60 .6 54 15 80 .7 82 16 82 .7 63 27 16 .1 17 26 79 .8 78 17 30 .8 30 26 36 .1 22 21 09 .0 46 27 39 .1 46 34 17 .5 05 13b 0:G10 MS Raw 0 2 4 6 8 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0.0 26 93 .1 79 87 9. 40 6 88 7. 38 3 13 02 .6 59 13 03 .6 41 89 5. 39 3 27 07 .1 84 20 64 .9 92 27 15 .1 57 27 25 .1 73 26 76 .1 38 28 45 .1 76 13a 0:L9 MS Raw 0.0 0.2 0.4 0.6 0.8 1.0 1.2 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 20. Haptoglobin 2 19. Retinol-binding protein 4 0 12 67 .6 41 17 08 .8 41 12 89 .6 24 87 9. 37 9 88 7. 37 0 83 0. 35 1 10 57 .3 58 10 58 .3 61 89 5. 38 5 88 9. 37 6 34 32 .6 29 85 2. 35 8 89 9. 38 6 91 9. 40 3 84 5. 35 4 29 62 .3 46 13 11 .5 98 15 80 .7 59 19 64 .9 38 16 84 .8 37 17 30 .8 15 23 78 .1 89 23 94 .1 80 34 46 .6 31 29 76 .3 61 24 15 .1 47 33 63 .5 64 33 49 .5 56 34 03 .5 54 33 61 .0 10 3-2 0:J17 MS Raw 0 1 2 3 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 21. Haptoglobin 2 089 5. 50 6 17 08 .9 58 15 80 .8 45 13 66 .7 94 14 39 .7 41 24 51 .3 33 18 57 .9 93 92 9. 54 5 33 49 .7 64 33 63 .7 28 96 8. 57 8 91 3. 51 1 13 11 .6 50 87 0. 52 8 17 97 .9 50 10 33 .5 70 18 72 .0 11 13 14 .7 27 85 6. 51 2 15 45 .7 80 86 9. 38 3 83 9. 35 8 83 8. 47 3 11 32 .5 79 92 7. 51 2 11 08 .5 86 91 7. 47 6 84 8. 44 3 14 61 .7 41 94 6. 50 4 11 38 .5 46 83 0. 45 2 99 8. 54 3 12 87 .6 88 13 13 .7 88 88 1. 48 4 11 49 .6 07 22 62 .1 78 13 94 .6 69 88 0. 45 7 14 97 .8 44 11 52 .5 64 87 3. 45 7 89 9. 46 6 22 76 .0 22 88 7. 43 6 12 05 .5 878 33 .4 23 12 86 .7 048 36 .4 65 98 6. 53 7 92 1. 50 3 13 33 .6 30 13 31 .7 40 18 80 .0 07 13 87 .7 09 96 4. 53 4 11 17 .5 41 94 5. 48 9 19 11 .0 05 15 29 .7 79 13 18 .6 22 12 74 .7 13 13 79 .2 05 15 22 .7 51 26 15 .8 14 16 93 .8 88 23 55 .5 77 26 30 .0 41 18 94 .9 97 23 69 .8 07 16 04 .9 87 18 12 .9 86 18 48 .9 07 20 18 .5 01 25 16 .5 97 33 34 .7 75 31 74 .5 68 15_15 0:G8 MS Raw 0.0 0.5 1.0 1.5 2.0 5x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 0 10 46 .5 62 87 0. 55 0 13 94 .6 47 82 9. 45 7 85 6. 52 6 17 08 .9 21 24 51 .2 66 95 1. 48 0 11 31 .6 56 15 22 .7 47 14 39 .7 33 13 57 .7 38 11 89 .6 15 89 7. 42 8 13 07 .7 06 11 07 .5 63 15 80 .8 27 86 4. 45 9 89 5. 46 2 99 3. 51 5 10 33 .5 29 12 34 .6 90 12 29 .6 77 10 65 .5 23 15 37 .7 59 83 8. 43 8 11 21 .5 82 10 37 .5 29 11 94 .6 24 90 8. 44 7 13 17 .6 46 13 66 .7 82 12 65 .6 52 83 3. 39 9 98 2. 45 4 16 12 .8 75 14 16 .6 38 10 60 .5 58 10 02 .5 49 84 6. 38 9 10 16 .5 46 96 2. 47 5 92 9. 49 3 93 4. 44 8 10 68 .5 24 11 57 .5 93 11 18 .5 27 14 11 .7 81 13 11 .6 28 14 34 .7 15 14 32 .6 30 14 50 .7 78 12 84 .5 92 14 64 .7 76 16 08 .7 96 17 51 .8 89 17 91 .7 80 18 57 .9 61 17 94 .8 37 18 51 .9 70 20 65 .9 97 31 40 .5 63 PD2_14 0:F16 MS Raw 0 2 4 6 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 22. Transthyretin 23. Haptoglobin 1 0.0 19 12 .9 49 18 92 .9 67 12 74 .7 20 21 77 .9 47 21 53 .9 57 16 84 .8 54 21 68 .9 62 18 48 .8 24 10 57 .3 75 14 00 .6 72 18 64 .8 03 91 5. 43 3 91 9. 40 1 21 55 .9 51 16 70 .7 95 16 12 .7 95 21 38 .9 55 17 91 .7 16 19 40 .9 42 12 90 .6 98 17 06 .8 06 21 16 .2 48 15 50 .7 15 16 39 .8 17 21 02 .2 19 19 34 .9 07 21 04 .9 85 2 18 5. 94 9 16 01 .5 49 23 42 .9 69 22 09 .9 84 18 97 .8 05 23 18 .9 57 22 00 .9 42 20 90 .9 55 21 93 .9 50 27 05 .1 55 27 17 .1 03 33 12 .2 95 6 0:G18 MS Raw 0.0 0.5 1.0 1.5 4x10 In te ns . [ a. u. ] 1000 1250 1500 1750 2000 2250 2500 2750 3000 3250 m/z 24. Serum amyloid A1 MASCOT Search Results Protein View: A2MG_HUMAN Alpha-2-macroglobulin OS=Homo sapiens OX=9606 GN=A2M PE=1 SV=3 Database: SwissProt Score: 95 Expect: 6e-06 Monoisotopic mass (Mr): 164613 Calculated pI: 6.03 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of A2MG_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 86 Mass values matched: 24 Protein sequence coverage: 20% Matched peptides shown in bold red. 1 MGKNKLLHPS LVLLLLVLLP TDASVSGKPQ YMVLVPSLLH TETTEKGCVL 51 LSYLNETVTV SASLESVRGN RSLFTDLEAE NDVLHCVAFA VPKSSSNEEV 101 MFLTVQVKGP TQEFKKRTTV MVKNEDSLVF VQTDKSIYKP GQTVKFRVVS 151 MDENFHPLNE LIPLVYIQDP KGNRIAQWQS FQLEGGLKQF SFPLSSEPFQ 201 GSYKVVVQKK SGGRTEHPFT VEEFVLPKFE VQVTVPKIIT ILEEEMNVSV 251 CGLYTYGKPV PGHVTVSICR KYSDASDCHG EDSQAFCEKF SGQLNSHGCF 301 YQQVKTKVFQ LKRKEYEMKL HTEAQIQEEG TVVELTGRQS SEITRTITKL 351 SFVKVDSHFR QGIPFFGQVR LVDGKGVPIP NKVIFIRGNE ANYYSNATTD 401 EHGLVQFSIN TTNVMGTSLT VRVNYKDRSP CYGYQWVSEE HEEAHHTAYL 451 VFSPSKSFVH LEPMSHELPC GHTQTVQAHY ILNGGTLLGL KKLSFYYLIM 501 AKGGIVRTGT HGLLVKQEDM KGHFSISIPV KSDIAPVARL LIYAVLPTGD 551 VIGDSAKYDV ENCLANKVDL SFSPSQSLPA SHAHLRVTAA PQSVCALRAV 601 DQSVLLMKPD AELSASSVYN LLPEKDLTGF PGPLNDQDNE DCINRHNVYI 651 NGITYTPVSS TNEKDMYSFL EDMGLKAFTN SKIRKPKMCP QLQQYEMHGP 701 EGLRVGFYES DVMGRGHARL VHVEEPHTET VRKYFPETWI WDLVVVNSAG 751 VAEVGVTVPD TITEWKAGAF CLSEDAGLGI SSTASLRAFQ PFFVELTMPY 801 SVIRGEAFTL KATVLNYLPK CIRVSVQLEA SPAFLAVPVE KEQAPHCICA 851 NGRQTVSWAV TPKSLGNVNF TVSAEALESQ ELCGTEVPSV PEHGRKDTVI 901 KPLLVEPEGL EKETTFNSLL CPSGGEVSEE LSLKLPPNVV EESARASVSV 951 LGDILGSAMQ NTQNLLQMPY GCGEQNMVLF APNIYVLDYL NETQQLTPEI 1001 KSKAIGYLNT GYQRQLNYKH YDGSYSTFGE RYGRNQGNTW LTAFVLKTFA 1051 QARAYIFIDE AHITQALIWL SQRQKDNGCF RSSGSLLNNA IKGGVEDEVT 1101 LSAYITIALL EIPLTVTHPV VRNALFCLES AWKTAQEGDH GSHVYTKALL 1151 AYAFALAGNQ DKRKEVLKSL NEEAVKKDNS VHWERPQKPK APVGHFYEPQ 1201 APSAEVEMTS YVLLAYLTAQ PAPTSEDLTS ATNIVKWITK QQNAQGGFSS 1251 TQDTVVALHA LSKYGAATFT RTGKAAQVTI QSSGTFSSKF QVDNNNRLLL 1301 QQVSLPELPG EYSMKVTGEG CVYLQTSLKY NILPEKEEFP FALGVQTLPQ 1351 TCDEPKAHTS FQISLSVSYT GSRSASNMAI VDVKMVSGFI PLKPTVKMLE 1401 RSNHVSRTEV SSNHVLIYLD KVSNQTLSLF FTVLQDVPVR DLKPAIVKVY 1451 DYYETDEFAI AEYNAPCSKD LGNA Unformatted sequence string: 1474 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 175 – 188 1604.7657 1603.7584 1603.8358 -48.3 0 R.IAQWQSFQLEGGLK.Q 189 – 204 1848.8113 1847.8040 1847.8730 -37.3 0 K.QFSFPLSSEPFQGSYK.V 215 – 228 1672.7917 1671.7844 1671.8508 -39.7 0 R.TEHPFTVEEFVLPK.F 290 – 305 1899.8224 1898.8151 1898.8734 -30.7 0 K.FSGQLNSHGCFYQQVK.T 320 – 338 2109.9903 2108.9831 2109.0702 -41.3 0 K.LHTEAQIQEEGTVVELTGR 339 – 345 820.4113 819.4040 819.4086 -5.59 0 R.QSSEITR.T 361 – 370 1148.5988 1147.5916 1147.6138 -19.4 0 R.QGIPFFGQVR.L 429 – 456 3338.4298 3337.4225 3337.4775 -16.5 0 R.SPCYGYQWVSEEHEEAHHT 532 – 539 828.4336 827.4263 827.4501 -28.7 0 K.SDIAPVAR.L 568 – 586 2048.9940 2047.9867 2048.0439 -27.9 0 K.VDLSFSPSQSLPASHAHLR 587 – 598 1272.6381 1271.6308 1271.6656 -27.4 0 R.VTAAPQSVCALR.A 688 – 704 2073.8831 2072.8758 2072.9230 -22.8 0 K.MCPQLQQYEMHGPEGLR.V 688 – 704 2089.8621 2088.8548 2088.9179 -30.2 0 K.MCPQLQQYEMHGPEGLR.V 705 – 715 1259.5404 1258.5331 1258.5652 -25.5 0 R.VGFYESDVMGR.G 720 – 732 1545.7465 1544.7393 1544.7947 -35.9 0 R.LVHVEEPHTETVR.K 788 – 804 2045.0104 2044.0031 2044.0492 -22.6 0 R.AFQPFFVELTMPYSVIR.G 788 – 804 2060.9944 2059.9872 2060.0441 -27.6 0 R.AFQPFFVELTMPYSVIR.G 935 – 945 1210.5984 1209.5911 1209.6353 -36.5 0 K.LPPNVVEESAR.A 1004 – 1014 1255.6135 1254.6063 1254.6357 -23.4 0 K.AIGYLNTGYQR.Q 1020 – 1031 1418.5643 1417.5570 1417.5899 -23.2 0 K.HYDGSYSTFGER.Y 1178 – 1190 1620.7833 1619.7761 1619.8168 -25.2 0 K.DNSVHWERPQKPK.A 1264 – 1271 886.4327 885.4254 885.4345 -10.2 0 K.YGAATFTR.T 1290 – 1297 1006.4483 1005.4410 1005.4628 -21.6 0 K.FQVDNNNR.L 1357 – 1373 1840.8629 1839.8556 1839.9115 -30.4 0 K.AHTSFQISLSVSYTGSR.S No match to: 826.2877, 842.3894, 848.2769, 850.3727, 861.0413, 879.3721, 888.3465, 889.3590, 895.3618, 947.4890, 1040.5396, 1058.3703, 1068.5270, 1106.5661, 1125.5697, 1131.5676, 1142.5836, 1170.5696, 1192.5295, 1277.5860, 1454.7129, 1513.7372, 1533.7041, 1587.7778, 1626.7851, 1636.7750, 1642.7680, 1658.7747, 1694.7954, 1831.7819, 1853.7943, 1870.8189, 1886.7886, 1892.7857, 1905.9787, 1913.8309, 1921.8281, 1935.8236, 1997.0044, 2008.3115, 2025.8719, 2031.8163, 2057.8285, 2067.0004, 2087.8984, 2095.8619, 2159.9278, 2163.0614, 2185.9147, 2259.9510, 2273.9541, 2281.9145, 2420.1989, 2497.1328, 2517.0739, 2562.0416, 2584.0188, 3199.4870, 3352.4173, 3360.3783, 3370.4030, 3375.3890 MASCOT Search Results Protein View: CERU_HUMAN Ceruloplasmin OS=Homo sapiens OX=9606 GN=CP PE=1 SV=1 Database: SwissProt Score: 112 Expect: 1.3e-07 Monoisotopic mass (Mr): 122983 Calculated pI: 5.44 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of CERU_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 66 Mass values matched: 19 Protein sequence coverage: 24% Matched peptides shown in bold red. 1 MKILILGIFL FLCSTPAWAK EKHYYIGIIE TTWDYASDHG EKKLISVDTE 51 HSNIYLQNGP DRIGRLYKKA LYLQYTDETF RTTIEKPVWL GFLGPIIKAE 101 TGDKVYVHLK NLASRPYTFH SHGITYYKEH EGAIYPDNTT DFQRADDKVY 151 PGEQYTYMLL ATEEQSPGEG DGNCVTRIYH SHIDAPKDIA SGLIGPLIIC 201 KKDSLDKEKE KHIDREFVVM FSVVDENFSW YLEDNIKTYC SEPEKVDKDN 251 EDFQESNRMY SVNGYTFGSL PGLSMCAEDR VKWYLFGMGN EVDVHAAFFH 301 GQALTNKNYR IDTINLFPAT LFDAYMVAQN PGEWMLSCQN LNHLKAGLQA 351 FFQVQECNKS SSKDNIRGKH VRHYYIAAEE IIWNYAPSGI DIFTKENLTA 401 PGSDSAVFFE QGTTRIGGSY KKLVYREYTD ASFTNRKERG PEEEHLGILG 451 PVIWAEVGDT IRVTFHNKGA YPLSIEPIGV RFNKNNEGTY YSPNYNPQSR 501 SVPPSASHVA PTETFTYEWT VPKEVGPTNA DPVCLAKMYY SAVDPTKDIF 551 TGLIGPMKIC KKGSLHANGR QKDVDKEFYL FPTVFDENES LLLEDNIRMF 601 TTAPDQVDKE DEDFQESNKM HSMNGFMYGN QPGLTMCKGD SVVWYLFSAG 651 NEADVHGIYF SGNTYLWRGE RRDTANLFPQ TSLTLHMWPD TEGTFNVECL 701 TTDHYTGGMK QKYTVNQCRR QSEDSTFYLG ERTYYIAAVE VEWDYSPQRE 751 WEKELHHLQE QNVSNAFLDK GEFYIGSKYK KVVYRQYTDS TFRVPVERKA 801 EEEHLGILGP QLHADVGDKV KIIFKNMATR PYSIHAHGVQ TESSTVTPTL 851 PGETLTYVWK IPERSGAGTE DSACIPWAYY STVDQVKDLY SGLIGPLIVC 901 RRPYLKVFNP RRKLEFALLF LVFDENESWY LDDNIKTYSD HPEKVNKDDE 951 EFIESNKMHA INGRMFGNLQ GLTMHVGDEV NWYLMGMGNE IDLHTVHFHG 1001 HSFQYKHRGV YSSDVFDIFP GTYQTLEMFP RTPGIWLLHC HVTDHIHAGM 1051 ETTYTVLQNE DTKSG Unformatted sequence string: 1065 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 44 – 62 2171.0270 2170.0197 2170.0654 -21.1 0 K.LISVDTEHSNIYLQNGPDR 69 – 81 1647.8086 1646.8013 1646.8304 -17.7 1 K.KALYLQYTDETFR.T 70 – 81 1519.7144 1518.7072 1518.7354 -18.6 0 K.ALYLQYTDETFR.T 111 – 128 2155.0509 2154.0436 2154.0647 -9.77 0 K.NLASRPYTFHSHGITYYK. 145 – 177 3693.5792 3692.5720 3692.6247 -14.3 1 R.ADDKVYPGEQYTYMLLATE 259 – 280 2455.0337 2454.0264 2454.0654 -15.9 0 R.MYSVNGYTFGSLPGLSMCA 259 – 280 2471.0401 2470.0328 2470.0603 -11.1 0 R.MYSVNGYTFGSLPGLSMCA 427 – 436 1203.5360 1202.5287 1202.5204 6.91 0 R.EYTDASFTNR.K 469 – 481 1371.7425 1370.7352 1370.7558 -15.0 0 K.GAYPLSIEPIGVR.F 482 – 500 2292.9955 2291.9882 2292.0195 -13.6 1 R.FNKNNEGTYYSPNYNPQSR 485 – 500 1903.7868 1902.7795 1902.8132 -17.7 0 K.NNEGTYYSPNYNPQSR.S 501 – 523 2531.2065 2530.1992 2530.2380 -15.3 0 R.SVPPSASHVAPTETFTYEW 563 – 570 811.4003 810.3930 810.4096 -20.5 0 K.GSLHANGR.Q 713 – 719 940.4193 939.4120 939.4232 -11.9 0 K.YTVNQCR.R 721 – 732 1431.6146 1430.6073 1430.6314 -16.8 0 R.QSEDSTFYLGER.T 786 – 793 1017.4482 1016.4409 1016.4563 -15.2 0 R.QYTDSTFR.V 865 – 887 2505.0933 2504.0860 2504.1166 -12.2 0 R.SGAGTEDSACIPWAYYSTV 888 – 901 1575.8252 1574.8179 1574.8490 -19.8 0 K.DLYSGLIGPLIVCR.R 1009 – 1031 2685.2344 2684.2271 2684.2469 -7.37 0 R.GVYSSDVFDIFPGTYQTLE No match to: 826.2964, 830.3540, 848.3008, 859.3449, 879.3752, 881.3009, 887.3666, 889.3685, 895.3748, 989.4849, 993.4508, 1057.3741, 1059.3650, 1131.5696, 1148.6031, 1220.4582, 1259.5374, 1289.6064, 1589.8379, 1613.7348, 1636.7803, 1672.8079, 1837.8858, 1848.8312, 1870.8327, 2045.0215, 2061.0078, 2073.8950, 2088.9267, 2090.9538, 2093.9340, 2111.0095, 2121.9353, 2140.0393, 2141.8642, 2177.0200, 2259.9564, 2430.0527, 2519.2360, 2553.1833, 2563.1952, 2632.2186, 2804.3878, 3416.6424, 3487.5071, 3707.5985, 3867.3625 MASCOT Search Results Protein View: ITIH4_HUMAN Inter-alpha-trypsin inhibitor heavy chain H4 OS=Homo sapiens OX=9606 GN=ITIH4 PE=1 SV=4 Database: SwissProt Score: 48 Expect: 0.33 Monoisotopic mass (Mr): 103521 Calculated pI: 6.51 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of ITIH4_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 26 Mass values matched: 6 Protein sequence coverage: 10% Matched peptides shown in bold red. 1 MKPPRPVRTC SKVLVLLSLL AIHQTTTAEK NGIDIYSLTV DSRVSSRFAH 51 TVVTSRVVNR ANTVQEATFQ MELPKKAFIT NFSMIIDGMT YPGIIKEKAE 101 AQAQYSAAVA KGKSAGLVKA TGRNMEQFQV SVSVAPNAKI TFELVYEELL 151 KRRLGVYELL LKVRPQQLVK HLQMDIHIFE PQGISFLETE STFMTNQLVD 201 ALTTWQNKTK AHIRFKPTLS QQQKSPEQQE TVLDGNLIIR YDVDRAISGG 251 SIQIENGYFV HYFAPEGLTT MPKNVVFVID KSGSMSGRKI QQTREALIKI 301 LDDLSPRDQF NLIVFSTEAT QWRPSLVPAS AENVNKARSF AAGIQALGGT 351 NINDAMLMAV QLLDSSNQEE RLPEGSVSLI ILLTDGDPTV GETNPRSIQN 401 NVREAVSGRY SLFCLGFGFD VSYAFLEKLA LDNGGLARRI HEDSDSALQL 451 QDFYQEVANP LLTAVTFEYP SNAVEEVTQN NFRLLFKGSE MVVAGKLQDR 501 GPDVLTATVS GKLPTQNITF QTESSVAEQE AEFQSPKYIF HNFMERLWAY 551 LTIQQLLEQT VSASDADQQA LRNQALNLSL AYSFVTPLTS MVVTKPDDQE 601 QSQVAEKPME GESRNRNVHS GSTFFKYYLQ GAKIPKPEAS FSPRRGWNRQ 651 AGAAGSRMNF RPGVLSSRQL GLPGPPDVPD HAAYHPFRRL AILPASAPPA 701 TSNPDPAVSR VMNMKIEETT MTTQTPAPIQ APSAILPLPG QSVERLCVDP 751 RHRQGPVNLL SDPEQGVEVT GQYEREKAGF SWIEVTFKNP LVWVHASPEH 801 VVVTRNRRSS AYKWKETLFS VMPGLKMTMD KTGLLLLSDP DKVTIGLLFW 851 DGRGEGLRLL LRDTDRFSSH VGGTLGQFYQ EVLWGSPAAS DDGRRTLRVQ 901 GNDHSATRER RLDYQEGPPG VEISCWSVEL Unformatted sequence string: 930 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 153 – 162 1203.7383 1202.7310 1202.7387 -6.36 1 R.RLGVYELLLK.V 538 – 546 1256.5818 1255.5745 1255.5808 -4.99 0 K.YIFHNFMER.L 669 – 688 2184.0738 2183.0666 2183.0912 -11.3 0 R.QLGLPGPPDVPDHAAYHPF 690 – 710 2045.0168 2044.0095 2044.0953 -42.0 0 R.LAILPASAPPATSNPDPAV 754 – 775 2415.1570 2414.1497 2414.1714 -8.98 0 R.QGPVNLLSDPEQGVEVTGQ 911 – 930 2334.0845 2333.0772 2333.0998 -9.67 1 R.RLDYQEGPPGVEISCWSVE No match to: 917.3328, 1057.3640, 1058.3722, 1060.3816, 1211.6404, 1250.6130, 1519.7444, 1641.7572, 1667.7294, 1671.8289, 1905.8039, 2056.0675, 2155.0575, 2167.0439, 2172.0641, 2259.9816, 2293.0024, 2348.1058, 2564.2746, 2701.2096 i MASCOT Search Results Protein View: TRFE_HUMAN Serotransferrin OS=Homo sapiens OX=9606 GN=TF PE=1 SV=3 Database: SwissProt Score: 152 Expect: 1.3e-11 Monoisotopic mass (Mr): 79294 Calculated pI: 6.81 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of TRFE_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 95 Mass values matched: 25 Protein sequence coverage: 33% Matched peptides shown in bold red. 1 MRLAVGALLV CAVLGLCLAV PDKTVRWCAV SEHEATKCQS FRDHMKSVIP 51 SDGPSVACVK KASYLDCIRA IAANEADAVT LDAGLVYDAY LAPNNLKPVV 101 AEFYGSKEDP QTFYYAVAVV KKDSGFQMNQ LRGKKSCHTG LGRSAGWNIP 151 IGLLYCDLPE PRKPLEKAVA NFFSGSCAPC ADGTDFPQLC QLCPGCGCST 201 LNQYFGYSGA FKCLKDGAGD VAFVKHSTIF ENLANKADRD QYELLCLDNT 251 RKPVDEYKDC HLAQVPSHTV VARSMGGKED LIWELLNQAQ EHFGKDKSKE 301 FQLFSSPHGK DLLFKDSAHG FLKVPPRMDA KMYLGYEYVT AIRNLREGTC 351 PEAPTDECKP VKWCALSHHE RLKCDEWSVN SVGKIECVSA ETTEDCIAKI 401 MNGEADAMSL DGGFVYIAGK CGLVPVLAEN YNKSDNCEDT PEAGYFAIAV 451 VKKSASDLTW DNLKGKKSCH TAVGRTAGWN IPMGLLYNKI NHCRFDEFFS 501 EGCAPGSKKD SSLCKLCMGS GLNLCEPNNK EGYYGYTGAF RCLVEKGDVA 551 FVKHQTVPQN TGGKNPDPWA KNLNEKDYEL LCLDGTRKPV EEYANCHLAR 601 APNHAVVTRK DKEACVHKIL RQQQHLFGSN VTDCSGNFCL FRSETKDLLF 651 RDDTVCLAKL HDRNTYEKYL GEEYVKAVGN LRKCSTSSLL EACTFRRP Unformatted sequence string: 698 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 24 – 37 1673.7763 1672.7690 1672.7991 -18.0 1 K.TVRWCAVSEHEATK.C 27 – 37 1317.5662 1316.5590 1316.5819 -17.4 0 R.WCAVSEHEATK.C 61 – 69 1125.5315 1124.5242 1124.5648 -36.1 1 K.KASYLDCIR.A 62 – 69 997.4542 996.4469 996.4698 -23.0 0 K.ASYLDCIR.A 122 – 132 1323.6261 1322.6188 1322.6401 -16.1 1 K.KDSGFQMNQLR.G 123 – 132 1211.5441 1210.5368 1210.5401 -2.70 0 K.DSGFQMNQLR.G + Oxidation (M) 144 – 162 2171.0721 2170.0649 2170.0881 -10.7 0 R.SAGWNIPIGLLYCDLPEPR.K 237 – 251 1881.9075 1880.9003 1880.8687 16.8 1 K.ADRDQYELLCLDNTR.K 240 – 251 1539.6842 1538.6769 1538.7035 -17.3 0 R.DQYELLCLDNTR.K 252 – 273 2549.2953 2548.2881 2548.2856 0.95 1 R.KPVDEYKDCHLAQVPSHTVVAR.S 298 – 310 1491.7170 1490.7097 1490.7518 -28.2 1 K.SKEFQLFSSPHGK.D 300 – 310 1276.6019 1275.5946 1275.6248 -23.7 0 K.EFQLFSSPHGK.D 328 – 343 1939.8748 1938.8675 1938.9219 -28.1 1 R.MDAKMYLGYEYVTAIR.N + Oxidation (M) 332 – 343 1478.7112 1477.7039 1477.7275 -16.0 0 K.MYLGYEYVTAIR.N 332 – 343 1494.6823 1493.6750 1493.7224 -31.7 0 K.MYLGYEYVTAIR.N + Oxidation (M) 363 – 371 1195.5207 1194.5134 1194.5352 -18.3 0 K.WCALSHHER.L 372 – 384 1521.6995 1520.6922 1520.7293 -24.4 1 R.LKCDEWSVNSVGK.I 454 – 464 1249.6074 1248.6001 1248.5986 1.22 0 K.SASDLTWDNLK.G Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 531 – 541 1283.5464 1282.5391 1282.5618 -17.7 0 K.EGYYGYTGAFR.C 572 – 587 1952.9306 1951.9233 1951.9309 -3.90 1 K.NLNEKDYELLCLDGTR.K 588 – 600 1586.7526 1585.7454 1585.7671 -13.7 0 R.KPVEEYANCHLAR.A 601 – 609 964.5057 963.4985 963.5250 -27.6 0 R.APNHAVVTR.K 647 – 659 1565.7719 1564.7646 1564.7919 -17.5 1 K.DLLFRDDTVCLAK.L 669 – 676 1000.4736 999.4664 999.4913 -24.9 0 K.YLGEEYVK.A 684 – 696 1531.6641 1530.6568 1530.6807 -15.6 0 K.CSTSSLLEACTFR.R No match to: 806.3029, 833.4825, 836.3980, 864.4605, 896.4401, 900.5118, 909.4053, 949.4210, 1012.5311, 1017.5199, 1029.5627, 1033.5502, 1036.4978, 1061.5471, 1148.5492, 1162.5366, 1189.5506, 1209.5260, 1213.5953, 1220.5509, 1255.6044, 1263.6182, 1338.6423, 1350.6488, 1352.6613, 1372.6586, 1386.7772, 1398.5728, 1418.5731, 1442.4144, 1448.6320, 1464.7217, 1514.6448, 1545.6818, 1552.7351, 1561.7047, 1579.7796, 1591.7917, 1600.7638, 1616.7480, 1620.7910, 1625.7720, 1646.6860, 1660.7235, 1703.8285, 1730.8471, 1759.8666, 1773.9937, 1811.9185, 1851.9095, 1883.8668, 1895.9519, 1925.8401, 1963.8930, 1966.9351, 2001.9016, 2070.9587, 2081.0165, 2084.9664, 2110.0527, 2154.9435, 2185.0887, 2203.0696, 2212.0231, 2225.0772, 2232.2346, 2246.2482, 2265.0848, 2377.1333, 2563.3055 MASCOT Search Results Protein View: A1AT_HUMAN Alpha-1-antitrypsin OS=Homo sapiens OX=9606 GN=SERPINA1 PE=1 SV=3 Database: SwissProt Score: 98 Expect: 3.1e-06 Monoisotopic mass (Mr): 46878 Calculated pI: 5.37 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of A1AT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 41 Mass values matched: 10 Protein sequence coverage: 27% Matched peptides shown in bold red. 1 MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI 51 TPNLAEFAFS LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI 101 LEGLNFNLTE IPEAQIHEGF QELLRTLNQP DSQLQLTTGN GLFLSEGLKL 151 VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ INDYVEKGTQ GKIVDLVKEL 201 DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV KVPMMKRLGM 251 FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL 301 ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP 351 LKLSKAVHKA VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMIE 401 QNTKSPLFMG KVVNPTQK Unformatted sequence string: 418 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 50 – 63 1641.8278 1640.8205 1640.8562 -21.8 0 K.ITPNLAEFAFSLYR.Q 150 – 160 1333.7102 1332.7029 1332.7653 -46.8 2 K.LVDKFLEDVKK.L 161 – 179 2185.9936 2184.9864 2185.0327 -21.2 1 K.LYHSEAFTVNFGDTEEAKK.Q 193 – 215 2757.4331 2756.4259 2756.5153 -32.4 2 K.IVDLVKELDRDTVFALVNYIFFK.G 199 – 215 2090.0489 2089.0416 2089.0884 -22.4 1 K.ELDRDTVFALVNYIFFK.G 216 – 225 1275.6584 1274.6511 1274.6771 -20.4 1 K.GKWERPFEVK.D 247 – 257 1403.6731 1402.6658 1402.6962 -21.7 1 K.RLGMFNIQHCK.K 299 – 306 1078.5059 1077.4986 1077.5203 -20.1 1 K.FLENEDRR.S 315 – 324 1110.5552 1109.5479 1109.5968 -44.1 0 K.LSITGTYDLK.S 325 – 334 1015.5856 1014.5784 1014.6073 -28.6 0 K.SVLGQLGITK.V No match to: 910.4660, 991.3701, 993.4395, 997.3814, 1019.3428, 1037.5817, 1047.3717, 1049.3650, 1057.3498, 1059.3851, 1132.5594, 1163.4403, 1166.4240, 1216.6149, 1220.4668, 1269.5595, 1307.6426, 1355.7092, 1663.7964, 1801.7064, 1825.8762, 1856.8854, 1877.9080, 1893.8750, 2112.0186, 2207.9732, 2596.2458, 2715.1913, 2731.2280, 3148.3646, 3180.3577 MASCOT Search Results Protein View: APOA4_HUMAN Apolipoprotein A-IV OS=Homo sapiens OX=9606 GN=APOA4 PE=1 SV=3 Database: SwissProt Score: 127 Expect: 4.1e-09 Monoisotopic mass (Mr): 45371 Calculated pI: 5.28 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of APOA4_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 72 Mass values matched: 17 Protein sequence coverage: 37% Matched peptides shown in bold red. 1 MFLKAVVLTL ALVAVAGARA EVSADQVATV MWDYFSQLSN NAKEAVEHLQ 51 KSELTQQLNA LFQDKLGEVN TYAGDLQKKL VPFATELHER LAKDSEKLKE 101 EIGKELEELR ARLLPHANEV SQKIGDNLRE LQQRLEPYAD QLRTQVNTQA 151 EQLRRQLTPY AQRMERVLRE NADSLQASLR PHADELKAKI DQNVEELKGR 201 LTPYADEFKV KIDQTVEELR RSLAPYAQDT QEKLNHQLEG LTFQMKKNAE 251 ELKARISASA EELRQRLAPL AEDVRGNLRG NTEGLQKSLA ELGGHLDQQV 301 EEFRRRVEPY GENFNKALVQ QMEQLRQKLG PHAGDVEGHL SFLEKDLRDK 351 VNSFFSTFKE KESQDKTLSL PELEQQQEQQ QEQQQEQVQM LAPLES Unformatted sequence string: 396 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 79 – 90 1439.8258 1438.8185 1438.7932 17.6 1 K.KLVPFATELHER.L 80 – 90 1311.7298 1310.7225 1310.6983 18.5 0 K.LVPFATELHER.L 124 – 134 1341.6693 1340.6620 1340.7160 -40.3 1 K.IGDNLRELQQR.L 135 – 143 1104.5936 1103.5863 1103.5611 22.8 0 R.LEPYADQLR.T 144 – 155 1443.7846 1442.7773 1442.7590 12.7 1 R.TQVNTQAEQLRR.Q 155 – 163 1132.6439 1131.6366 1131.6149 19.2 1 R.RQLTPYAQR.M 156 – 163 976.5419 975.5346 975.5138 21.4 0 R.QLTPYAQR.M 190 – 200 1300.6979 1299.6906 1299.6783 9.53 1 K.IDQNVEELKGR.L 199 – 209 1296.6480 1295.6407 1295.6510 -7.95 1 K.GRLTPYADEFK.V 201 – 209 1083.5429 1082.5356 1082.5284 6.67 0 R.LTPYADEFK.V 212 – 221 1258.6863 1257.6790 1257.6677 8.97 1 K.IDQTVEELRR.S 267 – 275 983.5670 982.5597 982.5447 15.2 0 R.LAPLAEDVR.G 288 – 304 1927.9782 1926.9709 1926.9435 14.2 0 K.SLAELGGHLDQQVEEFR.R 288 – 305 2084.0741 2083.0669 2083.0446 10.7 1 K.SLAELGGHLDQQVEEFRR.R 306 – 316 1352.6888 1351.6815 1351.6520 21.8 1 R.RVEPYGENFNK.A Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 317 – 326 1215.6702 1214.6629 1214.6441 15.5 0 K.ALVQQMEQLR.Q 329 – 345 1805.9297 1804.9225 1804.9108 6.47 0 K.LGPHAGDVEGHLSFLEK.D No match to: 805.3172, 806.3257, 810.3573, 812.4041, 820.3092, 826.2984, 836.2974, 842.2797, 844.2874, 848.2890, 864.2745, 879.3837, 881.3443, 887.3934, 889.3875, 896.4195, 920.4506, 923.5308, 942.4642, 945.5263, 959.5158, 980.5180, 993.4652, 997.4929, 1012.5058, 1049.3988, 1057.3844, 1058.3915, 1125.5786, 1160.6169, 1164.5703, 1176.6023, 1192.6034, 1220.5182, 1242.4985, 1257.6756, 1283.5845, 1338.6445, 1374.6673, 1461.7965, 1478.7556, 1495.7034, 1508.7822, 1565.8029, 1585.9006, 1607.8789, 1656.8503, 1721.8523, 1742.8298, 1752.9820, 1773.9928, 1827.9204, 1834.9345, 1849.9194, 2517.1152 MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 109 Expect: 2.6e-07 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 85 Mass values matched: 14 Protein sequence coverage: 33% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 162 – 170 923.5485 922.5413 922.5236 19.1 0 R.ILGGHLDAK.G 171 – 178 920.4769 919.4696 919.4552 15.7 0 K.GSFPWQAK.M 216 – 227 1290.7392 1289.7319 1289.7231 6.85 0 K.DIAPTLTLYVGK.K 216 – 228 1418.8465 1417.8392 1417.8181 14.9 1 K.DIAPTLTLYVGKK.Q 229 – 235 858.4943 857.4870 857.4858 1.37 0 K.QLVEIEK.V 254 – 261 959.5478 958.5405 958.5196 21.9 1 K.QKVSVNER.V 262 – 277 1834.9734 1833.9661 1833.9117 29.6 1 R.VMPICLPSKDYAEVGR.V 271 – 277 809.4102 808.4030 808.3715 38.9 0 K.DYAEVGR.V 278 – 286 980.5336 979.5263 979.4876 39.5 0 R.VGYVSGWGR.N 298 – 311 1707.8687 1706.8614 1706.8120 29.0 0 K.YVMLPVADQDQCIR.H 312 – 322 1274.6685 1273.6612 1273.6302 24.3 1 R.HYEGSTVPEKK.T 326 – 345 2172.0981 2171.0908 2171.0504 18.6 0 K.SPVGVQPILNEHTFCAGMSK.Y 380 – 391 1345.6648 1344.6575 1344.6384 14.2 0 K.SCAVAEYGVYVK.V 392 – 401 1203.6541 1202.6469 1202.6295 14.4 0 K.VTSIQDWVQK.T No match to: 806.3576, 820.3330, 826.3276, 828.3517, 829.3691, 831.3781, 836.3084, 842.2846, 844.3085, 848.3047, 860.3589, 864.2837, 880.4994, 887.3919, 888.3954, 889.4263, 896.4505, 899.3928, 926.5110, 942.4867, 945.5466, 958.4582, 961.5135, 974.5148, 976.5354, 994.5275, 1002.5139, 1012.5193, 1018.4898, 1057.4071, 1058.4071, 1060.4188, 1066.5704, 1069.4091, 1127.6075, 1160.6310, 1164.6120, 1168.5647, 1176.6222, 1182.6026, 1192.6205, 1215.6439, 1225.6653, 1234.7393, 1241.6298, 1296.6677, 1312.7538, 1328.7313, 1352.6991, 1367.6808, 1381.6896, 1440.8543, 1495.7257, 1532.8276, 1627.8444, 1659.8698, 1673.8749, 1721.8847, 1729.8525, 1737.8567, 1742.8559, 1745.8273, 1753.0072, 1814.9542, 1848.9763, 1856.9516, 2063.9987, 2095.9860, 2127.9858, 2194.1005, 2209.0901 MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 123 Expect: 1e-08 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 29 Mass values matched: 13 Protein sequence coverage: 33% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 162 – 170 923.5187 922.5114 922.5236 -13.2 0 R.ILGGHLDAK.G 171 – 178 920.4445 919.4372 919.4552 -19.5 0 K.GSFPWQAK.M 216 – 228 1418.7999 1417.7926 1417.8181 -17.9 1 K.DIAPTLTLYVGKK.Q 236 – 251 1794.9926 1793.9853 1794.0040 -10.4 0 K.VVLHPNYSQVDIGLIK.L 262 – 277 1834.9145 1833.9072 1833.9117 -2.47 1 R.VMPICLPSKDYAEVGR.V 262 – 277 1850.9047 1849.8974 1849.9066 -4.97 1 R.VMPICLPSKDYAEVGR.V + Oxidation (M) 278 – 286 980.4961 979.4888 979.4876 1.30 0 R.VGYVSGWGR.N 298 – 311 1707.8112 1706.8040 1706.8120 -4.72 0 K.YVMLPVADQDQCIR.H 298 – 311 1723.7788 1722.7715 1722.8069 -20.6 0 K.YVMLPVADQDQCIR.H + Oxidation (M) 312 – 322 1274.6241 1273.6169 1273.6302 -10.5 1 R.HYEGSTVPEKK.T 326 – 345 2172.0412 2171.0339 2171.0504 -7.59 0 K.SPVGVQPILNEHTFCAGMSK.Y 380 – 391 1345.6297 1344.6224 1344.6384 -11.9 0 K.SCAVAEYGVYVK.V 392 – 401 1203.6215 1202.6142 1202.6295 -12.8 0 K.VTSIQDWVQK.T No match to: 1012.4791, 1160.5889, 1164.5811, 1192.5747, 1234.6895, 1495.6757, 1509.6839, 1685.7507, 1721.8256, 1737.8151, 1742.7969, 1752.9457, 1774.9204, 1816.9740, 1845.0189, 1874.8669 MASCOT Search Results Protein View: APOA1_HUMAN Apolipoprotein A-I OS=Homo sapiens OX=9606 GN=APOA1 PE=1 SV=1 Database: SwissProt Score: 191 Expect: 1.6e-15 Monoisotopic mass (Mr): 30759 Calculated pI: 5.56 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of APOA1_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 98 Mass values matched: 23 Protein sequence coverage: 71% Matched peptides shown in bold red. 1 MKAAVLTLAV LFLTGSQARH FWQQDEPPQS PWDRVKDLAT VYVDVLKDSG 51 RDYVSQFEGS ALGKQLNLKL LDNWDSVTST FSKLREQLGP VTQEFWDNLE 101 KETEGLRQEM SKDLEEVKAK VQPYLDDFQK KWQEEMELYR QKVEPLRAEL 151 QEGARQKLHE LQEKLSPLGE EMRDRARAHV DALRTHLAPY SDELRQRLAA 201 RLEALKENGG ARLAEYHAKA TEHLSTLSEK AKPALEDLRQ GLLPVLESFK 251 VSFLSALEEY TKKLNTQ Unformatted sequence string: 267 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 35 – 47 1462.8248 1461.8175 1461.8443 -18.3 1 R.VKDLATVYVDVLK.D 37 – 47 1235.6406 1234.6334 1234.6809 -38.5 0 K.DLATVYVDVLK.D 48 – 64 1815.8664 1814.8591 1814.8435 8.60 1 K.DSGRDYVSQFEGSALGK.Q 52 – 64 1400.6506 1399.6433 1399.6620 -13.3 0 R.DYVSQFEGSALGK.Q 70 – 83 1612.7816 1611.7743 1611.7781 -2.31 0 K.LLDNWDSVTSTFSK.L 84 – 101 2202.1243 2201.1170 2201.1117 2.42 1 K.LREQLGPVTQEFWDNLEK.E 102 – 112 1323.6061 1322.5988 1322.6136 -11.2 1 K.ETEGLRQEMSK.D + Oxidation (M) 121 – 130 1252.6167 1251.6094 1251.6136 -3.28 0 K.VQPYLDDFQK.K 121 – 131 1380.7031 1379.6959 1379.7085 -9.16 1 K.VQPYLDDFQKK.W 131 – 140 1411.6716 1410.6643 1410.6601 2.93 1 K.KWQEEMELYR.Q 132 – 140 1283.5738 1282.5665 1282.5652 1.05 0 K.WQEEMELYR.Q 143 – 155 1467.7810 1466.7737 1466.7841 -7.06 1 K.VEPLRAELQEGAR.Q 148 – 155 873.4464 872.4392 872.4352 4.59 0 R.AELQEGAR.Q 165 – 173 1047.4918 1046.4845 1046.5066 -21.1 0 K.LSPLGEEMR.D + Oxidation (M) 165 – 175 1318.6222 1317.6149 1317.6347 -15.0 1 K.LSPLGEEMRDR.A + Oxidation (M) 185 – 195 1301.6477 1300.6404 1300.6411 -0.57 0 R.THLAPYSDELR.Q 185 – 197 1585.8026 1584.7954 1584.8008 -3.45 1 R.THLAPYSDELRQR.L 202 – 212 1157.6162 1156.6089 1156.6200 -9.56 1 R.LEALKENGGAR.L 213 – 219 831.4185 830.4112 830.4286 -21.0 0 R.LAEYHAK.A 220 – 230 1215.6289 1214.6216 1214.6143 6.04 0 K.ATEHLSTLSEK.A 231 – 239 1012.5782 1011.5709 1011.5713 -0.31 0 K.AKPALEDLR.Q 240 – 250 1230.6805 1229.6732 1229.7020 -23.4 0 R.QGLLPVLESFK.V 251 – 262 1386.6931 1385.6858 1385.7078 -15.9 0 K.VSFLSALEEYTK.K No match to: 806.3176, 820.3073, 826.2799, 842.2619, 855.5099, 859.3318, 861.3599, 869.3898, 879.3716, 887.3625, 889.3674, 979.5530, 993.5614, 1007.5780, 1057.3568, 1059.3701, 1158.5947, 1219.6572, 1226.5387, 1266.5546, 1268.6157, 1315.5574, 1405.6679, 1408.6805, 1484.8199, 1495.6729, 1580.8064, 1602.7801, 1632.7889, 1645.7979, 1653.7629, 1661.9146, 1675.9292, 1689.8772, 1705.8905, 1723.9390, 1733.9008, 1743.8551, 1765.8481, 1775.8486, 1797.9000, 1811.9147, 1819.9022, 1870.9907, 1878.9977, 1884.0078, 1946.0107, 1968.0051, 2037.9130, 2051.9329, 2065.0389, 2084.8869, 2094.9423, 2102.1329, 2108.9815, 2137.1176, 2141.0338, 2169.1222, 2206.1028, 2234.1261, 2303.1680, 2309.0439, 2323.0749, 2352.1872, 2380.2003, 2409.2121, 2423.2270, 2439.2305, 2450.2495, 2453.2422, 2466.2508, 2481.2564, 2494.2630, 2624.3350, 2677.3036 MASCOT Search Results Protein View: RET4_HUMAN Retinol-binding protein 4 OS=Homo sapiens OX=9606 GN=RBP4 PE=1 SV=3 Database: SwissProt Score: 70 Expect: 0.0019 Monoisotopic mass (Mr): 23337 Calculated pI: 5.76 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of RET4_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 13 Mass values matched: 5 Protein sequence coverage: 33% Matched peptides shown in bold red. 1 MKWVWALLLL AALGSGRAER DCRVSSFRVK ENFDKARFSG TWYAMAKKDP 51 EGLFLQDNIV AEFSVDETGQ MSATAKGRVR LLNNWDVCAD MVGTFTDTED 101 PAKFKMKYWG VASFLQKGND DHWIVDTDYD TYAVQYSCRL LNLDGTCADS 151 YSFVFSRDPN GLPPEAQKIV RQRQEELCLA RQYRLIVHNG YCDGRSERNL 201 L Unformatted sequence string: 201 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 31 – 37 879.4060 878.3987 878.4246 -29.5 1 K.ENFDKAR.F 118 – 139 2693.1786 2692.1713 2692.1136 21.4 0 K.GNDDHWIVDTDYDTYAVQYSCR.L 140 – 157 2064.9919 2063.9847 2063.9623 10.9 0 R.LLNLDGTCADSYSFVFSR.D 172 – 181 1302.6585 1301.6513 1301.6510 0.21 1 R.QRQEELCLAR.Q 185 – 195 1303.6413 1302.6341 1302.6139 15.5 0 R.LIVHNGYCDGR.S No match to: 803.3802, 887.3827, 895.3929, 2676.1381, 2707.1841, 2715.1574, 2725.1726, 2845.1762 MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 62 Expect: 0.011 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 52 Mass values matched: 8 Protein sequence coverage: 21% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 58 – 71 1580.7821 1579.7748 1579.7842 -5.96 1 K.LRTEGDGVYTLNDK.K 78 – 108 3432.6397 3431.6325 3431.5874 13.1 1 K.AVGDKLPECEADDGCPKPPEIAHGYVEHSVR.Y 83 – 108 2962.3669 2961.3596 2961.3385 7.12 0 K.LPECEADDGCPKPPEIAHGYVEHSVR.Y 117 – 131 1708.8695 1707.8622 1707.8428 11.4 1 K.LRTEGDGVYTLNNEK.Q 119 – 131 1439.6740 1438.6667 1438.6576 6.35 0 R.TEGDGVYTLNNEK.Q 119 – 136 2109.0464 2108.0391 2108.0174 10.3 1 R.TEGDGVYTLNNEKQWINK.A 154 – 161 895.4694 894.4621 894.4671 -5.66 0 K.NPANPVQR.I 298 – 311 1723.8001 1722.7928 1722.8069 -8.21 0 K.YVMLPVADQDQCIR.H + Oxidation (M) No match to: 897.3565, 899.3691, 901.3695, 903.3802, 993.4297, 1057.3665, 1058.3675, 1060.3780, 1069.3631, 1106.5854, 1161.5031, 1163.4464, 1198.6184, 1220.4959, 1230.5978, 1285.6265, 1303.6282, 1304.5689, 1317.6233, 1347.5372, 1505.7258, 1675.7664, 1682.7634, 1717.2898, 1730.8297, 2064.9828, 2078.9959, 2378.2329, 2410.2200, 2636.1222, 2649.1358, 2677.1207, 2679.8777, 2693.1589, 2707.1545, 2716.1171, 2725.1448, 2739.1459, 2976.3878, 3349.5677, 3363.5906, 3417.5049, 3446.6574, 3460.6543 MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 60 Expect: 0.022 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 38 Mass values matched: 6 Protein sequence coverage: 16% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 58 – 71 1580.7589 1579.7516 1579.7842 -20.6 1 K.LRTEGDGVYTLNDK.K 60 – 71 1311.5977 1310.5905 1310.5990 -6.54 0 R.TEGDGVYTLNDK.K 78 – 108 3432.6286 3431.6213 3431.5874 9.88 1 K.AVGDKLPECEADDGCPKPPEIAHGYVEHSVR.Y 83 – 108 2962.3461 2961.3389 2961.3385 0.11 0 K.LPECEADDGCPKPPEIAHGYVEHSVR.Y 117 – 131 1708.8414 1707.8342 1707.8428 -5.03 1 K.LRTEGDGVYTLNNEK.Q 271 – 277 809.3566 808.3493 808.3715 -27.5 0 K.DYAEVGR.V No match to: 800.3451, 801.3705, 803.3641, 805.3823, 807.3957, 819.3379, 825.3708, 830.3505, 845.3537, 852.3576, 879.3788, 887.3705, 889.3759, 895.3855, 899.3863, 919.4034, 1057.3584, 1058.3607, 1267.6405, 1289.6242, 1684.8375, 1730.8151, 1964.9382, 2378.1887, 2394.1799, 2415.1472, 2976.3607, 3349.5558, 3361.0096, 3363.5637, 3403.5543, 3446.6305 MASCOT Search Results Protein View: TTHY_HUMAN Transthyretin OS=Homo sapiens OX=9606 GN=TTR PE=1 SV=1 Database: SwissProt Score: 64 Expect: 0.0081 Monoisotopic mass (Mr): 15991 Calculated pI: 5.52 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of TTHY_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 65 Mass values matched: 6 Protein sequence coverage: 61% Matched peptides shown in bold red. 1 MASHRLLLLC LAGLVFVSEA GPTGTGESKC PLMVKVLDAV RGSPAINVAV 51 HVFRKAADDT WEPFASGKTS ESGELHGLTT EEEFVEGIYK VEIDTKSYWK 101 ALGISPFHEH AEVVFTANDS GPRRYTIAAL LSPYSYSTTA VVTNPKE Unformatted sequence string: 147 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 30 – 41 1416.6379 1415.6306 1415.7629 -93.4 1 K.CPLMVKVLDAVR.G + Oxidation (M) 42 – 54 1366.7816 1365.7743 1365.7517 16.5 0 R.GSPAINVAVHVFR.K 55 – 68 1522.7470 1521.7397 1521.7100 19.6 1 R.KAADDTWEPFASGK.T 56 – 68 1394.6468 1393.6395 1393.6150 17.6 0 K.AADDTWEPFASGK.T 69 – 96 3140.5632 3139.5559 3139.5085 15.1 1 K.TSESGELHGLTTEEEFVEGIYKVEIDTK.S 101 – 123 2451.2658 2450.2586 2450.1979 24.8 0 K.ALGISPFHEHAEVVFTANDSGPR.R No match to: 820.3859, 822.3767, 824.3827, 829.4568, 833.3989, 838.4381, 846.3893, 856.5263, 864.4590, 870.5501, 895.4615, 897.4285, 908.4467, 929.4930, 934.4477, 951.4799, 962.4752, 982.4537, 993.5146, 1002.5493, 1016.5464, 1033.5290, 1037.5285, 1046.5618, 1060.5584, 1065.5226, 1068.5239, 1107.5627, 1118.5271, 1121.5817, 1131.6564, 1157.5929, 1189.6149, 1194.6238, 1229.6773, 1234.6904, 1265.6520, 1284.5922, 1307.7057, 1311.6276, 1317.6457, 1357.7376, 1411.7805, 1432.6300, 1434.7149, 1439.7327, 1450.7778, 1464.7760, 1537.7588, 1580.8267, 1608.7958, 1612.8750, 1708.9214, 1751.8887, 1791.7799, 1794.8374, 1851.9701, 1857.9608, 2065.9970 MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens GN=HP PE=1 SV=1 Database: SwissProt Score: 64 Expect: 0.008 Nominal mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 81 Mass values matched: 10 Protein sequence coverage: 19% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort peptides by nmlkji Residue Number nmlkj Increasing Mass nmlkj Decreasing Mass Show predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 58 – 71 1580.8454 1579.8381 1579.7842 34.1 1 K.LRTEGDGVYTLNDK.K 60 – 71 1311.6498 1310.6425 1310.5990 33.2 0 R.TEGDGVYTLNDK.K 60 – 72 1439.7408 1438.7335 1438.6940 27.5 1 R.TEGDGVYTLNDKK.Q 113 – 118 856.5123 855.5050 855.4603 52.3 1 K.NYYKLR.T 117 – 131 1708.9585 1707.9512 1707.8428 63.5 1 K.LRTEGDGVYTLNNEK.Q 137 – 153 1857.9926 1856.9853 1856.9124 39.2 1 K.AVGDKLPECEAVCGKPK.N 142 – 153 1387.7088 1386.7015 1386.6635 27.4 0 K.LPECEAVCGKPK.N 154 – 161 895.5063 894.4990 894.4671 35.7 0 K.NPANPVQR.I 228 – 235 986.5370 985.5298 985.5808 -51.8 1 K.KQLVEIEK.V 312 – 322 1274.7130 1273.7058 1273.6302 59.3 1 R.HYEGSTVPEKK.T No match to: 820.3984, 822.4516, 824.4226, 830.4521, 833.4226, 836.4647, 838.4726, 839.3575, 848.4429, 869.3826, 870.5281, 873.4573, 880.4565, 881.4837, 887.4361, 899.4657, 913.5113, 917.4760, 921.5032, 927.5120, 929.5450, 945.4888, 946.5040, 964.5340, 968.5782, 998.5432, 1033.5704, 1108.5862, 1117.5409, 1132.5793, 1138.5461, 1149.6068, 1152.5638, 1205.5870, 1286.7040, 1287.6882, 1313.7879, 1314.7267, 1318.6225, 1331.7396, 1333.6300, 1366.7941, 1379.2050, 1394.6691, 1461.7405, 1497.8438, 1522.7508, 1529.7794, 1545.7805, 1604.9875, 1693.8878, 1797.9501, 1812.9856, 1848.9073, 1872.0110, 1880.0068, 1894.9975, 1911.0051, 2018.5006, 2262.1784, 2276.0221, 2355.5774, 2369.8066, 2451.3325, 2516.5968, 2615.8135, 2630.0413, 3174.5680, 3334.7752, 3349.7638, 3363.7276 MASCOT Search Results Protein View: SAA1_HUMAN Serum amyloid A-1 protein OS=Homo sapiens OX=9606 GN=SAA1 PE=1 SV=1 Database: SwissProt Score: 63 Expect: 0.01 Monoisotopic mass (Mr): 13581 Calculated pI: 6.28 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of SAA1_HUMAN against nr. Search parameters Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Mass values searched: 39 Mass values matched: 5 Protein sequence coverage: 55% Matched peptides shown in bold red. 1 MKLLTGLVFC SLVLGVSSRS FFSFLGEAFD GARDMWRAYS DMREANYIGS 51 DKYFHARGNY DAAKRGPGGV WAAEAISDAR ENIQRFFGHG AEDSLADQAA 101 NEWGRSGKDP NHFRPAGLPE KY Unformatted sequence string: 122 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Start – End Observed Mr(expt) Mr(calc) ppm M Peptide 20 – 33 1550.7146 1549.7073 1549.7202 -8.28 0 R.SFFSFLGEAFDGAR.D 20 – 37 2138.9551 2137.9478 2137.9680 -9.45 1 R.SFFSFLGEAFDGARDMWR.A 44 – 57 1670.7946 1669.7874 1669.7848 1.52 1 R.EANYIGSDKYFHAR.G 65 – 80 1612.7949 1611.7876 1611.8117 -14.9 1 K.RGPGGVWAAEAISDAR.E 86 – 105 2177.9472 2176.9400 2176.9562 -7.48 0 R.FFGHGAEDSLADQAANEWGR.S No match to: 915.4331, 919.4010, 1057.3753, 1274.7195, 1290.6976, 1400.6719, 1601.5489, 1639.8174, 1684.8545, 1706.8060, 1791.7161, 1848.8244, 1864.8034, 1892.9673, 1897.8046, 1912.9490, 1934.9070, 1940.9421, 2090.9553, 2102.2195, 2104.9846, 2116.2476, 2153.9570, 2155.9506, 2168.9625, 2185.9487, 2193.9496, 2200.9420, 2209.9841, 2318.9566, 2342.9688, 2705.1554, 2717.1025, 3312.2954 11 48 .6 25 17 5. 20 5 24 5. 25 7 10 14 .4 18 29 9. 31 5 70 .1 10 21 7. 22 0 18 6. 16 2 13 2. 14 7 11 24 .4 46 83 3. 48 9 10 94 .1 29 57 7. 40 8 33 3. 26 9 10 98 .4 34 11 22 .4 63 39 2. 36 2 37 9. 29 8 86 .1 85 85 0. 55 3 45 9. 41 9 25 7. 27 1 11 30 .2 52 58 9. 39 5 27 4. 35 7 10 1. 13 2 15 8. 13 9 96 3. 24 3 39 6. 38 6 60 6. 54 1 12 0. 11 0 38 5. 34 0 40 2. 35 3 44 2. 31 8 12 9. 18 8 54 3. 33 6 1_1148 0:E7 LIFT 1148.6210 0 2000 4000 6000In te ns . [ a. u. ] 200 400 600 800 1000 1200 m/z 1. Alpha-2-macroglobulin_ 1148.62 21 20 .2 58 21 55 .0 60 11 0. 18 1 20 27 .1 52 65 0. 31 7 70 .1 57 22 8. 29 1 36 2. 37 1 54 2. 48 4 21 09 .8 67 20 0. 22 5 13 6. 16 6 96 6. 46 4 11 78 .6 24 29 9. 32 2 42 8. 23 8 38 3. 23 3 96 8. 60 2 61 8. 51 3 10 22 .7 35 88 1. 63 5 57 4. 50 8 77 4. 53 8 31 0. 38 9 19 81 .2 63 51 4. 40 4 87 5. 79 7 47 3. 41 7 38 6. 47 1 11 05 .7 24 74 4. 60 8 27 1. 28 0 12 52 .6 11 18 57 .2 47 18 57 .5 64 2_2155 0:E8 LIFT 2155.0510 0 1 2 3 4x10 In te ns . [ a. u. ] 250 500 750 1000 1250 1500 1750 2000 2250 2500 m/z 2. Ceruloplasmin_2155.05 21 53 .8 07 99 8. 75 7 13 09 .8 15 21 57 .0 44 21 08 .5 10 21 45 .1 64 70 .2 42 11 0. 23 5 12 10 .7 37 21 37 .2 36 61 1. 58 2 86 1. 57 5 16 18 .7 89 10 96 .8 81 18 62 .8 42 11 58 .6 98 71 9. 63 5 19 25 .4 38 79 0. 50 3 70 2. 57 5 20 38 .7 14 18 68 .6 72 4_2184 0:F7 LIFT 2184.0740 0.00 0.25 0.50 0.75 1.00 1.25 1.50 4x10 In te ns . [ a. u. ] 250 500 750 1000 1250 1500 1750 2000 2250 2500 m/z 3. Inter-alpha-trypsin inhibitor heavy chain H4_2184.07 17 25 .9 30 14 92 .8 95 18 58 .4 23 14 24 .8 94 17 5. 30 3 18 62 .7 04 18 63 .8 23 16 95 .9 55 18 56 .0 75 39 0. 46 4 56 9. 57 5 12 69 .9 06 11 19 .8 47 18 50 .2 69 17 56 .0 54 18 03 .0 56 86 .2 51 18 22 .0 22 45 8. 49 0 17 90 .9 81 70 .2 20 11 2. 25 7 58 6. 60 4 18 81 .9 16 10 04 .7 94 16 80 .1 85 15 39 .8 99 16 79 .0 73 35 7. 46 2 1 84 6. 99 2 18 53 .1 42 18 10 .3 01 17 07 .9 24 47 0. 53 6 63 3. 64 5 76 8. 70 9 13 94 .9 04 15 22 .9 30 18 09 .3 69 92 8. 72 5 33 9. 42 0 17 94 .2 33 86 1. 67 0 44 1. 46 5 10 1. 23 7 37 3. 45 4 11 84 .8 53 17 68 .9 85 27 6. 38 2 15 09 .7 11 73 2. 61 5 89 1. 74 34 52 .4 87 18 19 .7 68 18 39 .1 70 77 2. 76 6 11 33 .8 37 85 0. 70 2 12 96 .8 98 11 5. 27 2 44 .2 27 12 9. 26 4 34 3. 47 8 13 3. 25 7 87 8. 64 0 61 8. 57 3 48 8. 47 1 72 .2 08 13 6. 25 7 15 8. 25 6 50 5. 51 8 72 1. 73 4 77 8. 64 8 10 2. 20 0 60 .2 20 25 9. 48 0 99 1. 69 8 13 49 .7 13 96 3. 71 1 55 8. 60 7 88 .2 66 10 76 .6 25 13 77 .9 59 12 36 .8 10 31 5. 39 5 74 9. 57 7 5_1881 0:F8 LIFT 1881.9080 0.0 0.5 1.0 1.5 2.0 2.5 4x10 In te ns . [ a. u. ] 250 500 750 1000 1250 1500 1750 2000 m/z 7. Serotransferrin_1881.91 17 5. 24 9 32 5. 34 2 21 2. 25 7 90 3. 59 3 52 5. 42 5 39 6. 38 1 53 8. 45 8 70 .1 46 16 19 .5 04 32 1. 32 3 42 6. 39 4 75 6. 53 7 33 8. 33 0 14 27 .8 70 45 1. 41 6 15 98 .2 13 34 8. 31 5 49 7. 43 6 11 2. 19 8 86 .1 97 16 22 .7 32 68 5. 51 753 9. 40 0 73 9. 54 7 67 2. 46 7 12 0. 21 1 62 6. 47 5 61 0. 50 0 74 3. 50 8 11 03 .6 5341 9. 34 6 29 7. 34 7 13 6. 22 4 16 41 .8 27 88 6. 58 4 95 7. 58 3 12 16 .7 77 13 13 .7 61 21 5. 26 0 52 1. 42 6 64 4. 50 8 11 04 .6 56 87 .2 30 39 8. 38 3 21 9. 24 7 50 8. 43 5 20 1. 25 7 10 32 .6 62 30 6. 30 7 80 4. 28 6 84 4. 51 8 71 5. 53 46 68 .4 87 44 .0 80 31 3. 39 1 36 4. 31 5 58 2. 39 9 51 1. 46 3 56 4. 42 3 18 7. 26 5 47 7. 36 3 78 2. 67 2 71 1. 51 6 53 6. 96 4 16_828 0:K8 LIFT 1641.8280 0.0 0.5 1.0 1.5 4x10 In te ns . [ a. u. ] 200 400 600 800 1000 1200 1400 1600 1800 m/z 12. Alpha-1-antitrypsin_ 1641.83 12 24 .7 71 13 33 .2 47 38 5. 43 1 13 52 .6 94 12 07 .7 95 12 13 .2 05 21 1. 35 1 13 35 .7 88 70 .1 71 36 8. 40 1 12 74 .9 39 83 1. 47 7 11 2. 23 1 26 1. 34 1 70 8. 43 9 96 8. 53 7 35 7. 42 6 25 6. 32 7 22 8. 35 7 13 05 .6 32 44 7. 41 5 52 2. 43 3 13 2. 18 9 84 8. 45 6 61 7. 46 6 87 .2 32 12 96 .9 49 80 3. 52 6 23 9. 32 7 10 47 .6 24 78 6. 45 2 11 78 .7 69 13 15 .7 18 10 64 .6 54 34 0. 41 672 .1 78 12 9. 24 7 13 6. 24 1 60 0. 51 4 91 7. 47 6 10 2. 18 4 90 0. 49 5 24 4. 32 5 69 1. 34 0 65 1. 43 3 65 7. 41 9 10 80 .5 39 40 8. 38 1 12 0. 25 7 11 96 .7 04 50 5. 45 3 69 0. 38 7 10 97 .6 02 87 1. 49 0 67 4. 36 0 12 0. 56 4 10_1352 0:G8 LIFT 1352.6890 0.0 0.5 1.0 1.5 2.0 4x10 In te ns . [ a. u. ] 200 400 600 800 1000 1200 1400 1600 m/z 13. Apolipoprotein A-IV_ 1352.69 20 33 .4 01 15 91 .1 32 21 48 .4 99 18 16 .1 67 20 47 .7 23 22 6. 40 6 12 67 .9 46 49 8. 58 3 33 41 .9 96 20 55 .4 10 94 6. 79 0 11 54 .9 24 10 83 .8 67 33 90 .0 48 30 91 .2 49 34 05 .1 91 33 84 .1 32 33 77 .2 92 34 09 .7 84 13c_3432 0:J7 LIFT 3432.6189 0 2000 4000 6000 8000 In te ns . [ a. u. ] 500 1000 1500 2000 2500 3000 3500 4000 m/z 21. Haptoglobin 2_3432.62 81 9. 61 1 57 6. 52 5 26 3. 33 5 17 5. 27 0 39 4. 40 3 16 59 .1 11 16 84 .9 54 38 3. 39 9 16 81 .5 75 62 6. 53 4 16 16 .9 97 59 0. 55 4 16 89 .4 74 16 88 .0 60 16 72 .4 73 70 .1 38 86 .1 69 12 01 .7 91 84 8. 39 5 28 8. 37 8 23 5. 31 6 13 6. 19 2 26 8. 36 1 69 1. 54 0 83 3. 69 5 44 8. 46 1 43 1. 43 9 93 4. 62 4 50 7. 48 4 72 .2 16 91 7. 59 7 10 05 .7 07 10 1. 16 0 55 9. 50 2 11 84 .7 44 67 4. 51 1 13 3. 24 3 17 07 .8 14 80 2. 57 1 27 1. 40 0 60 4. 57 0 10 4. 14 9 74 6. 53 6 67 5. 63 3 98 8. 61 1 13 92 .4 84 47 9. 52 5 11_1707 0:H7 LIFT 1707.8690 0.0 0.2 0.4 0.6 0.8 1.0 4x10 In te ns . [ a. u. ] 200 400 600 800 1000 1200 1400 1600 1800 2000 m/z 14. Haptoglobin _1707.87 11 47 .6 46 16 41 .7 34 14 25 .6 96 22 92 .1 00 53 9. 36 7 26 20 .9 30 72 5. 44 8 69 7. 45 8 10 46 .5 89 24 07 .2 93 51 1. 34 6 83 8. 57 3 81 2. 56 5 15 40 .6 48 26 62 .8 65 26 67 .2 08 26 93 .1 08 26 59 .3 97 1_2693 0:E17 LIFT 2693.1040 0 2000 4000 6000 In te ns . [ a. u. ] 500 1000 1500 2000 2500 3000 m/z 19. Retinol-binding protein 4_2693.10 41 6. 53 7 71 6. 70 4 11 0. 24 1 15 00 .0 82 20 36 .2 52 11 62 .9 66 70 .1 72 53 1. 56 2 25 5. 39 9 26 7. 31 6 81 7. 77 9 86 .2 41 33 8. 45 7 13 63 .0 16 32 9. 49 4 62 8. 61 9 20 10 .1 70 31 2. 48 3 10 63 .9 09 96 4. 81 7 95 2. 88 0 64 5. 67 8 12 89 .9 79 44 2. 53 7 43 7. 53 3 10 89 .9 34 22 95 .2 40 38 2. 56 4 51 1. 58 8 82 3. 82 6 46 7. 53 1 24 2. 39 4 51 4. 59 1 16 29 .1 13 56 6. 66 4 18 5. 31 3 79 5. 84 6 13 89 .0 99 35 5. 50 6 84 8. 76 7 27 2. 43 7 36 6. 48 8 53 6. 65 1 12 92 .0 07 12 0. 26 4 17 66 .0 89 60 4. 58 2 14 88 .1 86 30 0. 41 57 4. 32 9 20 9. 29 7 17 5. 32 1 22 67 .2 37 2451_14_Paciente 18_ 0:K7 LIFT 2451.2000 0 1 2 3 4x10 In te ns . [ a. u. ] 500 1000 1500 2000 2500 m/z 22. Transthyretin_2451.20 67 2. 23 9 15 80 .6 00 50 0. 19 5 25 3. 13 0 86 .0 71 57 5. 22 5 50 4. 15 6 60 0. 25 6 15 62 .5 88 13 20 .5 14 16 83 .4 25 71 8. 27 4 80 0. 31 6 61 7. 23 6 88 1. 32 6 10 37 .3 67 71 2. 25 0 12 22 .4 99 47 2. 20 6 70 .0 44 11 2. 06 3 17 5. 09 3 81 1. 29 1 13 36 .5 46 15 34 .6 63 39 0. 12 8 96 3. 35 5 27 0. 12 5 15 36 .6 64 13 6. 06 4 35 4. 16 4 28 8. 13 3 60 5. 97 3 14 33 .5 84 16 88 .5 14 16 81 .1 36 32 9. 11 5 34 3. 17 2 48 7. 13 3 78 3. 30 3 13 06 .5 30 18 5. 08 4 53 1. 19 5 55 7. 20 6 11 77 .5 28 40 3. 08 1 26 5. 08 6 16 64 .2 36 65 5. 19 7 16 86 .3 57 16 46 .0 12 22 9. 07 2 12 9. 05 7 12 09 .4 00 64 4. 24 0 54 2. 18 2 72 9. 25 8 37 1. 15 2 16 49 .2 39 23 1. 05 7 46 0. 11 8 35 9. 07 2 70 1. 26 6 55 9. 16 9 11 5. 07 1 10 64 .4 13 82 8. 28 8 36 9. 14 4 69 4. 20 5 99 1. 36 6 43 0. 13 0 21 3. 09 9 74 .2 53 37 3. 10 3 14 05 .6 51 41 4. 20 8 12 05 .4 41 16 58 .4 47 78 6. 27 3 10 92 .3 89 10 48 .4 12 97 4. 33 2 10 2. 05 6 88 7. 41 9 27 6. 11 0 18 7. 06 5 25 8. 10 6 59 .0 24 14 7. 05 8 12 0. 05 1 1708.912_3PD 0:E8 LIFT 1708.9120 0.00 0.25 0.50 0.75 1.00 1.25 1.50 5x10 In te ns . [ a. u. ] 200 400 600 800 1000 1200 1400 1600 1800 2000 m/z 23. Haptoglobin 1_1708.91 MASCOT Search Results Protein View: A2MG_HUMAN Alpha-2-macroglobulin OS=Homo sapiens OX=9606 GN=A2M PE=1 SV=3 Database: SwissProt Score: 63 Monoisotopic mass (Mr): 164613 Calculated pI: 6.03 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of A2MG_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 0% Matched peptides shown in bold red. 1 MGKNKLLHPS LVLLLLVLLP TDASVSGKPQ YMVLVPSLLH TETTEKGCVL 51 LSYLNETVTV SASLESVRGN RSLFTDLEAE NDVLHCVAFA VPKSSSNEEV 101 MFLTVQVKGP TQEFKKRTTV MVKNEDSLVF VQTDKSIYKP GQTVKFRVVS 151 MDENFHPLNE LIPLVYIQDP KGNRIAQWQS FQLEGGLKQF SFPLSSEPFQ 201 GSYKVVVQKK SGGRTEHPFT VEEFVLPKFE VQVTVPKIIT ILEEEMNVSV 251 CGLYTYGKPV PGHVTVSICR KYSDASDCHG EDSQAFCEKF SGQLNSHGCF 301 YQQVKTKVFQ LKRKEYEMKL HTEAQIQEEG TVVELTGRQS SEITRTITKL 351 SFVKVDSHFR QGIPFFGQVR LVDGKGVPIP NKVIFIRGNE ANYYSNATTD 401 EHGLVQFSIN TTNVMGTSLT VRVNYKDRSP CYGYQWVSEE HEEAHHTAYL 451 VFSPSKSFVH LEPMSHELPC GHTQTVQAHY ILNGGTLLGL KKLSFYYLIM 501 AKGGIVRTGT HGLLVKQEDM KGHFSISIPV KSDIAPVARL LIYAVLPTGD 551 VIGDSAKYDV ENCLANKVDL SFSPSQSLPA SHAHLRVTAA PQSVCALRAV 601 DQSVLLMKPD AELSASSVYN LLPEKDLTGF PGPLNDQDNE DCINRHNVYI 651 NGITYTPVSS TNEKDMYSFL EDMGLKAFTN SKIRKPKMCP QLQQYEMHGP 701 EGLRVGFYES DVMGRGHARL VHVEEPHTET VRKYFPETWI WDLVVVNSAG 751 VAEVGVTVPD TITEWKAGAF CLSEDAGLGI SSTASLRAFQ PFFVELTMPY 801 SVIRGEAFTL KATVLNYLPK CIRVSVQLEA SPAFLAVPVE KEQAPHCICA 851 NGRQTVSWAV TPKSLGNVNF TVSAEALESQ ELCGTEVPSV PEHGRKDTVI 901 KPLLVEPEGL EKETTFNSLL CPSGGEVSEE LSLKLPPNVV EESARASVSV 951 LGDILGSAMQ NTQNLLQMPY GCGEQNMVLF APNIYVLDYL NETQQLTPEI 1001 KSKAIGYLNT GYQRQLNYKH YDGSYSTFGE RYGRNQGNTW LTAFVLKTFA 1051 QARAYIFIDE AHITQALIWL SQRQKDNGCF RSSGSLLNNA IKGGVEDEVT 1101 LSAYITIALL EIPLTVTHPV VRNALFCLES AWKTAQEGDH GSHVYTKALL 1151 AYAFALAGNQ DKRKEVLKSL NEEAVKKDNS VHWERPQKPK APVGHFYEPQ 1201 APSAEVEMTS YVLLAYLTAQ PAPTSEDLTS ATNIVKWITK QQNAQGGFSS 1251 TQDTVVALHA LSKYGAATFT RTGKAAQVTI QSSGTFSSKF QVDNNNRLLL 1301 QQVSLPELPG EYSMKVTGEG CVYLQTSLKY NILPEKEEFP FALGVQTLPQ 1351 TCDEPKAHTS FQISLSVSYT GSRSASNMAI VDVKMVSGFI PLKPTVKMLE 1401 RSNHVSRTEV SSNHVLIYLD KVSNQTLSLF FTVLQDVPVR DLKPAIVKVY 1451 DYYETDEFAI AEYNAPCSKD LGNA Unformatted sequence string: 1474 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expe 1 361 – 370 1148.6210 1147.6137 1147.6138 -0.11 0 63 1.8e- n MASCOT Search Results Protein View: CERU_HUMAN Ceruloplasmin OS=Homo sapiens OX=9606 GN=CP PE=1 SV=1 Database: SwissProt Score: 80 Monoisotopic mass (Mr): 122983 Calculated pI: 5.44 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of CERU_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 1% Matched peptides shown in bold red. 1 MKILILGIFL FLCSTPAWAK EKHYYIGIIE TTWDYASDHG EKKLISVDTE 51 HSNIYLQNGP DRIGRLYKKA LYLQYTDETF RTTIEKPVWL GFLGPIIKAE 101 TGDKVYVHLK NLASRPYTFH SHGITYYKEH EGAIYPDNTT DFQRADDKVY 151 PGEQYTYMLL ATEEQSPGEG DGNCVTRIYH SHIDAPKDIA SGLIGPLIIC 201 KKDSLDKEKE KHIDREFVVM FSVVDENFSW YLEDNIKTYC SEPEKVDKDN 251 EDFQESNRMY SVNGYTFGSL PGLSMCAEDR VKWYLFGMGN EVDVHAAFFH 301 GQALTNKNYR IDTINLFPAT LFDAYMVAQN PGEWMLSCQN LNHLKAGLQA 351 FFQVQECNKS SSKDNIRGKH VRHYYIAAEE IIWNYAPSGI DIFTKENLTA 401 PGSDSAVFFE QGTTRIGGSY KKLVYREYTD ASFTNRKERG PEEEHLGILG 451 PVIWAEVGDT IRVTFHNKGA YPLSIEPIGV RFNKNNEGTY YSPNYNPQSR 501 SVPPSASHVA PTETFTYEWT VPKEVGPTNA DPVCLAKMYY SAVDPTKDIF 551 TGLIGPMKIC KKGSLHANGR QKDVDKEFYL FPTVFDENES LLLEDNIRMF 601 TTAPDQVDKE DEDFQESNKM HSMNGFMYGN QPGLTMCKGD SVVWYLFSAG 651 NEADVHGIYF SGNTYLWRGE RRDTANLFPQ TSLTLHMWPD TEGTFNVECL 701 TTDHYTGGMK QKYTVNQCRR QSEDSTFYLG ERTYYIAAVE VEWDYSPQRE 751 WEKELHHLQE QNVSNAFLDK GEFYIGSKYK KVVYRQYTDS TFRVPVERKA 801 EEEHLGILGP QLHADVGDKV KIIFKNMATR PYSIHAHGVQ TESSTVTPTL 851 PGETLTYVWK IPERSGAGTE DSACIPWAYY STVDQVKDLY SGLIGPLIVC 901 RRPYLKVFNP RRKLEFALLF LVFDENESWY LDDNIKTYSD HPEKVNKDDE 951 EFIESNKMHA INGRMFGNLQ GLTMHVGDEV NWYLMGMGNE IDLHTVHFHG 1001 HSFQYKHRGV YSSDVFDIFP GTYQTLEMFP RTPGIWLLHC HVTDHIHAGM 1051 ETTYTVLQNE DTKSG Unformatted sequence string: 1065 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expe 1 111 – 128 2155.0510 2154.0437 2154.0647 -9.72 0 80 2.3e- MASCOT Search Results Protein View: ITIH4_HUMAN Inter-alpha-trypsin inhibitor heavy chain H4 OS=Homo sapiens OX=9606 GN=ITIH4 PE=1 SV=4 Database: SwissProt Score: 50 Monoisotopic mass (Mr): 103521 Calculated pI: 6.51 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of ITIH4_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 2% Matched peptides shown in bold red. 1 MKPPRPVRTC SKVLVLLSLL AIHQTTTAEK NGIDIYSLTV DSRVSSRFAH 51 TVVTSRVVNR ANTVQEATFQ MELPKKAFIT NFSMIIDGMT YPGIIKEKAE 101 AQAQYSAAVA KGKSAGLVKA TGRNMEQFQV SVSVAPNAKI TFELVYEELL 151 KRRLGVYELL LKVRPQQLVK HLQMDIHIFE PQGISFLETE STFMTNQLVD 201 ALTTWQNKTK AHIRFKPTLS QQQKSPEQQE TVLDGNLIIR YDVDRAISGG 251 SIQIENGYFV HYFAPEGLTT MPKNVVFVID KSGSMSGRKI QQTREALIKI 301 LDDLSPRDQF NLIVFSTEAT QWRPSLVPAS AENVNKARSF AAGIQALGGT 351 NINDAMLMAV QLLDSSNQEE RLPEGSVSLI ILLTDGDPTV GETNPRSIQN 401 NVREAVSGRY SLFCLGFGFD VSYAFLEKLA LDNGGLARRI HEDSDSALQL 451 QDFYQEVANP LLTAVTFEYP SNAVEEVTQN NFRLLFKGSE MVVAGKLQDR 501 GPDVLTATVS GKLPTQNITF QTESSVAEQE AEFQSPKYIF HNFMERLWAY 551 LTIQQLLEQT VSASDADQQA LRNQALNLSL AYSFVTPLTS MVVTKPDDQE 601 QSQVAEKPME GESRNRNVHS GSTFFKYYLQ GAKIPKPEAS FSPRRGWNRQ 651 AGAAGSRMNF RPGVLSSRQL GLPGPPDVPD HAAYHPFRRL AILPASAPPA 701 TSNPDPAVSR VMNMKIEETT MTTQTPAPIQ APSAILPLPG QSVERLCVDP 751 RHRQGPVNLL SDPEQGVEVT GQYEREKAGF SWIEVTFKNP LVWVHASPEH 801 VVVTRNRRSS AYKWKETLFS VMPGLKMTMD KTGLLLLSDP DKVTIGLLFW 851 DGRGEGLRLL LRDTDRFSSH VGGTLGQFYQ EVLWGSPAAS DDGRRTLRVQ 901 GNDHSATRER RLDYQEGPPG VEISCWSVEL Unformatted sequence string: 930 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 669 – 688 2184.0740 2183.0667 2183.0912 -11.2 0 50 0.00025 1 U R.QLGLPGPPDVPDHAAYHPFR.R MASCOT Search Results Protein View: TRFE_HUMAN Serotransferrin OS=Homo sapiens OX=9606 GN=TF PE=1 SV=3 Database: SwissProt Score: 91 Monoisotopic mass (Mr): 79294 Calculated pI: 6.81 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of TRFE_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 2% Matched peptides shown in bold red. 1 MRLAVGALLV CAVLGLCLAV PDKTVRWCAV SEHEATKCQS FRDHMKSVIP 51 SDGPSVACVK KASYLDCIRA IAANEADAVT LDAGLVYDAY LAPNNLKPVV 101 AEFYGSKEDP QTFYYAVAVV KKDSGFQMNQ LRGKKSCHTG LGRSAGWNIP 151 IGLLYCDLPE PRKPLEKAVA NFFSGSCAPC ADGTDFPQLC QLCPGCGCST 201 LNQYFGYSGA FKCLKDGAGD VAFVKHSTIF ENLANKADRD QYELLCLDNT 251 RKPVDEYKDC HLAQVPSHTV VARSMGGKED LIWELLNQAQ EHFGKDKSKE 301 FQLFSSPHGK DLLFKDSAHG FLKVPPRMDA KMYLGYEYVT AIRNLREGTC 351 PEAPTDECKP VKWCALSHHE RLKCDEWSVN SVGKIECVSA ETTEDCIAKI 401 MNGEADAMSL DGGFVYIAGK CGLVPVLAEN YNKSDNCEDT PEAGYFAIAV 451 VKKSASDLTW DNLKGKKSCH TAVGRTAGWN IPMGLLYNKI NHCRFDEFFS 501 EGCAPGSKKD SSLCKLCMGS GLNLCEPNNK EGYYGYTGAF RCLVEKGDVA 551 FVKHQTVPQN TGGKNPDPWA KNLNEKDYEL LCLDGTRKPV EEYANCHLAR 601 APNHAVVTRK DKEACVHKIL RQQQHLFGSN VTDCSGNFCL FRSETKDLLF 651 RDDTVCLAKL HDRNTYEKYL GEEYVKAVGN LRKCSTSSLL EACTFRRP Unformatted sequence string: 698 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 237 – 251 1881.9080 1880.9007 1880.8687 17.1 1 91 2e-08 1 U K.ADRDQYELLCLDNTR.K MASCOT Search Results Protein View: A1AT_HUMAN Alpha-1-antitrypsin OS=Homo sapiens OX=9606 GN=SERPINA1 PE=1 SV=3 Database: SwissProt Score: 99 Monoisotopic mass (Mr): 46878 Calculated pI: 5.37 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of A1AT_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 3% Matched peptides shown in bold red. 1 MPSSVSWGIL LLAGLCCLVP VSLAEDPQGD AAQKTDTSHH DQDHPTFNKI 51 TPNLAEFAFS LYRQLAHQSN STNIFFSPVS IATAFAMLSL GTKADTHDEI 101 LEGLNFNLTE IPEAQIHEGF QELLRTLNQP DSQLQLTTGN GLFLSEGLKL 151 VDKFLEDVKK LYHSEAFTVN FGDTEEAKKQ INDYVEKGTQ GKIVDLVKEL 201 DRDTVFALVN YIFFKGKWER PFEVKDTEEE DFHVDQVTTV KVPMMKRLGM 251 FNIQHCKKLS SWVLLMKYLG NATAIFFLPD EGKLQHLENE LTHDIITKFL 301 ENEDRRSASL HLPKLSITGT YDLKSVLGQL GITKVFSNGA DLSGVTEEAP 351 LKLSKAVHKA VLTIDEKGTE AAGAMFLEAI PMSIPPEVKF NKPFVFLMIE 401 QNTKSPLFMG KVVNPTQK Unformatted sequence string: 418 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 50 – 63 1641.8280 1640.8207 1640.8562 -21.6 0 99 3.8e-09 1 U K.ITPNLAEFAFSLYR.Q MASCOT Search Results Protein View: APOA4_HUMAN Apolipoprotein A-IV OS=Homo sapiens OX=9606 GN=APOA4 PE=1 SV=3 Database: SwissProt Score: 76 Monoisotopic mass (Mr): 45371 Calculated pI: 5.28 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of APOA4_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 2% Matched peptides shown in bold red. 1 MFLKAVVLTL ALVAVAGARA EVSADQVATV MWDYFSQLSN NAKEAVEHLQ 51 KSELTQQLNA LFQDKLGEVN TYAGDLQKKL VPFATELHER LAKDSEKLKE 101 EIGKELEELR ARLLPHANEV SQKIGDNLRE LQQRLEPYAD QLRTQVNTQA 151 EQLRRQLTPY AQRMERVLRE NADSLQASLR PHADELKAKI DQNVEELKGR 201 LTPYADEFKV KIDQTVEELR RSLAPYAQDT QEKLNHQLEG LTFQMKKNAE 251 ELKARISASA EELRQRLAPL AEDVRGNLRG NTEGLQKSLA ELGGHLDQQV 301 EEFRRRVEPY GENFNKALVQ QMEQLRQKLG PHAGDVEGHL SFLEKDLRDK 351 VNSFFSTFKE KESQDKTLSL PELEQQQEQQ QEQQQEQVQM LAPLES Unformatted sequence string: 396 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 306 – 316 1352.6890 1351.6817 1351.6520 22.0 1 76 7.9e-07 1 U R.RVEPYGENFNK.A MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 75 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 3% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 298 – 311 1707.8690 1706.8617 1706.8120 29.1 0 75 9.6e-07 1 U K.YVMLPVADQDQCIR.H MASCOT Search Results Protein View: RET4_HUMAN Retinol-binding protein 4 OS=Homo sapiens OX=9606 GN=RBP4 PE=1 SV=3 Database: SwissProt Score: 65 Monoisotopic mass (Mr): 23337 Calculated pI: 5.76 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of RET4_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 10% Matched peptides shown in bold red. 1 MKWVWALLLL AALGSGRAER DCRVSSFRVK ENFDKARFSG TWYAMAKKDP 51 EGLFLQDNIV AEFSVDETGQ MSATAKGRVR LLNNWDVCAD MVGTFTDTED 101 PAKFKMKYWG VASFLQKGND DHWIVDTDYD TYAVQYSCRL LNLDGTCADS 151 YSFVFSRDPN GLPPEAQKIV RQRQEELCLA RQYRLIVHNG YCDGRSERNL 201 L Unformatted sequence string: 201 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 118 – 139 2693.1040 2692.0967 2692.1136 -6.28 0 65 1.3e-06 1 U K.GNDDHWIVDTDYDTYAVQYSCR.L MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 70 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 7% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 78 – 108 3432.6189 3431.6116 3431.5874 7.05 1 70 1.2e-06 1 U K.AVGDKLPECEADDGCPKPPEIAHGYVEHSVR.Y MASCOT Search Results Protein View: TTHY_HUMAN Transthyretin OS=Homo sapiens OX=9606 GN=TTR PE=1 SV=1 Database: SwissProt Score: 189 Monoisotopic mass (Mr): 15991 Calculated pI: 5.52 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of TTHY_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 15% Matched peptides shown in bold red. 1 MASHRLLLLC LAGLVFVSEA GPTGTGESKC PLMVKVLDAV RGSPAINVAV 51 HVFRKAADDT WEPFASGKTS ESGELHGLTT EEEFVEGIYK VEIDTKSYWK 101 ALGISPFHEH AEVVFTANDS GPRRYTIAAL LSPYSYSTTA VVTNPKE Unformatted sequence string: 147 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 101 – 123 2451.2000 2450.1927 2450.1979 -2.10 0 189 2.5e-18 1 U K.ALGISPFHEHAEVVFTANDSGPR.R MASCOT Search Results Protein View: HPT_HUMAN Haptoglobin OS=Homo sapiens OX=9606 GN=HP PE=1 SV=1 Database: SwissProt Score: 91 Monoisotopic mass (Mr): 45861 Calculated pI: 6.13 Taxonomy: Homo sapiens Sequence similarity is available as an NCBI BLAST search of HPT_HUMAN against nr. Search parameters MS data file: DATA.TXT Enzyme: Trypsin: cuts C-term side of KR unless next residue is P. Fixed modifications: Carbamidomethyl (C) Variable modifications: Oxidation (M) Protein sequence coverage: 3% Matched peptides shown in bold red. 1 MSALGAVIAL LLWGQLFAVD SGNDVTDIAD DGCPKPPEIA HGYVEHSVRY 51 QCKNYYKLRT EGDGVYTLND KKQWINKAVG DKLPECEADD GCPKPPEIAH 101 GYVEHSVRYQ CKNYYKLRTE GDGVYTLNNE KQWINKAVGD KLPECEAVCG 151 KPKNPANPVQ RILGGHLDAK GSFPWQAKMV SHHNLTTGAT LINEQWLLTT 201 AKNLFLNHSE NATAKDIAPT LTLYVGKKQL VEIEKVVLHP NYSQVDIGLI 251 KLKQKVSVNE RVMPICLPSK DYAEVGRVGY VSGWGRNANF KFTDHLKYVM 301 LPVADQDQCI RHYEGSTVPE KKTPKSPVGV QPILNEHTFC AGMSKYQEDT 351 CYGDAGSAFA VHDLEEDTWY ATGILSFDKS CAVAEYGVYV KVTSIQDWVQ 401 KTIAEN Unformatted sequence string: 406 residues (for pasting into other applications). Sort by nmlkji residue number nmlkj increasing mass nmlkj decreasing mass Show nmlkji matched peptides only nmlkj predicted peptides also Query Start – End Observed Mr(expt) Mr(calc) ppm M Score Expect Rank U Peptide 1 117 – 131 1708.9120 1707.9047 1707.8428 36.3 1 91 3.1e-08 1 U K.LRTEGDGVYTLNNEK.Q