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ARTICLE IN PRESSG ModelMEDCLI-6252; No. of Pages 7 Medicina Clínica xxx (xxxx) xxx–xxxw w w.elsev ier .es /medic inac l in ica Original article Value of increased soluble suppressor tumorigenicity biomarker 2 (sST2) on admission as an indicator of severity in patients with COVID-19 María Arnaldos-Carrilloa,b, José Antonio Noguera-Velascoa,b,c, Isabel M. Martínez-Ardil g, Alejandro Riquelme-Pérezb,f, Iria Cebreiros-Lópeza,b,c, Álvaro Hernández-Vicenteb, José Antonio Ros-Lucash,i, Amjad Khank, Antoni Bayes-Genís f,j, Domingo Pascual-Figalb,c,d,e,f,∗ a Clinical Laboratory Service, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain b Universidad de Murcia, Murcia, Spain c IMIB Pascual Parrilla, Murcia, Spain d Cardiology Service, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain e Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain f CIBER Cardiovascular, Madrid, Spain g Family and Community Medicine, Servicio Murciano de Salud, Murcia, Spain h Pneumology Service, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain i Universidad Católica de Murcia (UCAM), Murcia, Spain j Heart Institute, Hospital Universitari Germans Trias i Pujol, Badalona, Barcelona, Spain k Nuffield Division of Clinical Laboratory Sciences (NDCLS), Radcliffe Department of Medicine, John Radcliffe hospital, University of Oxford, Oxford, UK a r t i c l e i n f o Article history: Received 21 November 2022 Accepted 1 April 2023 Available online xxx Keywords: COVID-19 pneumonia sST2 Risk stratification a b s t r a c t Background: Soluble suppressor of tumorigenicity-2 (sST2) is a biomarker for heart failure and pulmonary injury. We hypothesize that sST2 could help predict severity of SARS-CoV-2 infections. Methods: sST2 was analyzed in patients consecutively admitted for SARS-CoV-2 pneumonia. Other prog- nostic markers were also measured. In-hospital complications were registered, including death, ICU admission, and respiratory support requirements. Results: 495 patients were studied (53% male, age: 57.6 ± 17.6). At admission, median sST2 concentrations was 48.5 ng/mL [IQR, 30.6–83.1 ng/mL] and correlated with male gender, older age, comorbidities, other severity biomarkers, and respiratory support requirements. sST2 levels were higher in patients who died (n = 45, 9.1%) (45.6 [28.0, 75.9] ng/mL vs. 144 [82.6, 319] ng/mL, p < 0.001) and those admitted to ICU (n = 46, 9.3%) (44.7 [27.5, 71.3] ng/mL vs. 125 [69.0, 262] ng/mL, p < 0.001). sST2 levels > 210 ng/mL were a strong predictor of complicated in-hospital courses, with higher risk of death (OR, 39.3, CI95% 15.9, 103) and death/ICU (OR 38.3, CI95% 16.3–97.5) after adjusting for all other risk factors. The addition of sST2 enhanced the predictive capacity of mortality risk models. Conclusions: sST2 represents a robust severity predictor in COVID-19 and could be an important tool for identifying at-risk patients who may benefit from closer follow-up and specific therapies. © 2023 Elsevier Espan˜a, S.L.U. All rights reserved. Valor del aumento del biomarcador supresor soluble de tumorigenicidad 2 o indicador de gravedad en pacientes con COVID-19(sST2) al ingreso comPalabras clave: Neumonía por COVID-19 sST2 Estratificación de riesgo r e s u m e n Antecedentes: El supresor soluble de tumorigenicidad 2 (sST2) es un biomarcador de insuficiencia cardiaca y dan˜o pulmonar. Nuestra hipótesis es que la determinación de sST2 al ingreso podría ayudar a predecir la gravedad de la infección por SARS-CoV-2. ∗ Corresponding author. E-mail address: dpascual@um.es (D. Pascual-Figal). https://doi.org/10.1016/j.medcli.2023.04.005 0025-7753/© 2023 Elsevier Espan˜a, S.L.U. All rights reserved. Please cite this article as: M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al., Value of increased soluble sup- pressor tumorigenicity biomarker 2 (sST2) on admission as an indicator of severity in patients with COVID-19, Med Clin (Barc)., https://doi.org/10.1016/j.medcli.2023.04.005 ARTICLE IN PRESSG ModelMEDCLI-6252; No. of Pages 7 M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al. Medicina Clínica xxx (xxxx) xxx–xxx Métodos: Se analizó la concentración de sST2 en pacientes ingresados por neumonía por SARS-CoV-2, junto con otros biomarcadores pronósticos conocidos. Asimismo, se registraron las complicaciones durante la estancia hospitalaria, incluidas la muerte, el ingreso en Unidad de Cuidados Intensivos (UCI) y los requerimientos de soporte respiratorio. Resultados: Se estudiaron 495 pacientes (53% hombres, edad 57,6 ± 17,6). Al ingreso, la mediana de la concentración de sST2 fue 48,5 ng/mL (índice intercuartílico [IQR] 30,6-83,1 ng/mL) y correlacionó con el género masculino, una mayor edad, comorbilidades, otros biomarcadores de gravedad, así como necesidad de soporte respiratorio. Los niveles de sST2 fueron mayores en pacientes que fallecieron (n = 45, 9,1%) (45,6 [28,0, 75,9] ng/mL vs. 144 [82,6, 319] ng/mL, p < 0,001) y aquellos que requirieron ingreso en UCI (n = 46, 9,3%) (44,7 [27,5, 71,3] ng/mL vs. 125 [69,0, 262] ng/mL, p < 0,001). Así, los valores de sST2 > 210 ng/mL se han demostrado como un fuerte predictor de complicaciones, con un mayor riesgo de fallecimiento (odds ratio [OR], 39,3, intervalo de confianza [IC] 95% 15,9, 103) y fallecimiento o ingreso en UCI (OR 38,3, IC 95% 16,3-97,5), tras el ajuste por todos los demás factores de riesgo. La adición de la determinación de los niveles de sST2 mejoró la potencia predictiva de los modelos de riesgo desarrollados. Conclusiones: El sST2 representa un predictor robusto de la gravedad en pacientes con COVID-19 y podría convertirse en una herramienta importante para la identificación de pacientes en riesgo que podrían beneficiarse de un mayor seguimiento y terapias específicas. M S p 2 S d ( a P t a t p s B N f t A ( o a V s T a i w b w h ( or extracorporeal membrane oxygenation), clinical deterioration in terms of WHO and Sequential Organ Failure Assessment (SOFA)Introduction The novel coronavirus SARS CoV-2 (Severe Acute Respiratory Syndrome coronavirus 2) is the causative agent of coronavirus disease 2019 (COVID-19), which has spread rapidly, leading the global population to pandemic conditions and individual countries to healthcare collapse.1,2 The clinical manifestations of COVID-19 range from patients with asymptomatic infection or mild upper respiratory tract involvement to patients with severe pneumonia that develops into acute respiratory distress syndrome (ARDS) with multiorgan involvement and/or failure and requires admission to intensive care units (ICUs) for treatment with high-flow oxygen or mechanical ventilation.3 Immune and inflammatory responses in SARS-CoV-2 infection determine the disease severity of the disease, causing the infamous cytokine storm. Tumorigenicity suppressor 2 (ST2) is part of the interleukin-1 receptor (IL-1) family of proteins and is involved in inflammatory processes such as autoimmune diseases, sepsis, asthma, or pul- monary fibrosis. ST2 is the receptor for interleukin-33, a cytokine of a type known as “alarmins” that are secreted by most cells in the body in response to cellular damage or stress. Two biologically relevant isoforms of ST2 are produced by alternative splicing of the ST2 gene: a transmembrane isoform (ST2L) and a soluble and cir- culating isoform (sST2).4,5 The IL-33/ST2L signaling pathway serves a protective function by reducing the inflammatory response and preventing fibrosis, cardiomyocyte hypertrophy, and apoptosis in patients with heart failure (HF).6 By contrast, sST2 acts as a decoy receptor, as it binds more avidly than ST2L to IL-33, thereby reduc- ing the ST2L/IL-33 interaction and attenuating the favorable effects of the ST2L isoform. In addition to the cardiological effects of sST2, different stud- ies have provided sufficient evidence to support a role for sST2 in several lung diseases, such as asthma, ARDS, and pulmonary fibrosis.7–9 Alladina et al.10 described an association between sST2 levels and the need for mechanical ventilation, reintubation, and mortality in patients with ARDS. Likewise, Pascual-Figal et al. demonstrated that, in decompensated heart failure (HF), sST2 orig- inates mostly in the lungs, specifically in type II pneumocytes, in response to myocardial damage.11 Their evidence came via the use of an experimental model of HF in rodents, in which they observed an increase in the expression of sST2 in the lungs. The aim of this study was to determine the clinical performance of sST2 as a biomarker for the prognosis of severe COVID-19 disease, defined as admission to the ICU, as well as death. s d 2© 2023 Elsevier Espan˜a, S.L.U. Todos los derechos reservados. aterials and methods tudy population This prospective observational study was conducted on 495 atients hospitalized from July 2020 to March 2021 with SARS CoV- pneumonia at the Virgen de la Arrixaca tertiary hospital in Murcia, pain. We included adult patients (over 18 years of age) with a iagnosis of COVID-19 confirmed by a polymerase chain reaction PCR) test of nasopharyngeal exudate who had undergone a blood nalysis at admission and for whom a blood sample was available. atients admitted to our hospital by referral from other health cen- ers were excluded. Even though the vaccination fin Spain started t the end of December 2020, just one of the patients had received he first dose of Pfizer vaccine before his hospitalization for COVID neumonia. Biological samples were obtained at the time of hospital admis- ion and were collected, processed, and transferred by the Network iobank of the Region of Murcia, BIOBANC-MUR, registered in the ational Registry of Biobanks (registration number: B.0000859), ollowing standard working procedures and with the approval of he Ethics Committee and the Scientific Committee. Virgen de la rrixaca University Hospital Clinical Research Ethics Committee CEIm), internal code: 2020-6-1-4HCUVA. Patients provided signed informed consent for the conservation f the samples and their use in clinical research. The study was pproved by the hospital’s ethics committee. ariables and clinical events The demographic variables of the patients at the time of admis- ion and the clinical events during hospitalization were collected. hese variables included clinical risk factors, such as gender, age, nd the presence of comorbidities, as well as analytical parameters, ncluding hematological parameters (blood count and coagulation ith D-dimer), markers of renal, hepatic, and cardiac function, and iomarkers of inflammation. The WHO Clinical Progression Scale as also scored for each patient. Clinical events occurring during ospitalization were defined as death, intensive care requirements high-flow non-invasive oxygen, invasive mechanical ventilation,cales, development of respiratory distress, and need for oxygen at ischarge. ING Model a t t R S w i 4 a f f ( w t d s D ( c p o w h f ( C l h r a ( s s h r D a n r t p p n w 8 sARTICLEMEDCLI-6252; No. of Pages 7 M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al. Analytical methods Blood samples were taken at the time of hospital admission in vacuum tubes for serum, with lithium heparin as an anticoagulant for biochemical determinations, with citrate for coagulation tests, and with EDTA K3 for blood counts. Blood counts were performed on an XN 4000i hematology (Sysmex®) analyzer. The determination of the basic coagulation and D-dimer tests were performed in an ACL TOP 350 analyzer (Werfen®). In accordance with the hospital’s COVID-19 protocol, a panel of biochemical determinations was established and con- ducted using a Cobas® 8000 system (Roche Diagnostics®). This panel included biochemical assessments of renal function (urea and creatinine), liver function (AST, ALT, GGT, and bilirubin), inflamma- tion (using ferritin, high-sensitive C-reactive protein [hs-CRP], and interleukin-6), and heart damage (using NT-proBNP and Troponin T). Soluble ST2 was measured using the SEQUENT-IATM ST2 assay test. This is a homogeneous quantitative monoclonal immunoassay from Critical Diagnostics® that uses turbidimetry for the measure- ment of serum-soluble ST2. The assay was adapted to be measured in a Cobas® c502 analyzer (Roche Diagnostics). According to the manufacturer, the minimum detectable value is 10.8 ng/ml, being the lower linear limit 15 ng/ml and the upper linear limit 300 ng/ml. A concentration of 35 ng/mL is between the 90th and 95th per- centile of the normal population. It can be measured both in serum and EDTA anticoagulated plasma, and the test duration is around 15 min. Thus, the sST2 measurement can be performed at the emer- gency laboratory in half an hour. Statistical analysis Descriptive analyses were performed based on the quartiles of sST2. For numerical variables, means with standard deviations or median with interquartile ranges were calculated depending on their normal distribution. For categorical variables, their frequen- cies were calculated as percentages. The numerical variables were compared using ANOVA or the Kruskal–Wallis test (in cases of non-normality). The categorical variables were compared using the chi-square test or Fisher’s exact test (for variables complying with the Cochran Q test). Receiver operating characteristic (ROC) curves were analyzed to study the capacity of numerical values of sST2 marker to predict death and ICU events. To compare both of them, DeLong test was used. Logistic regressions were adjusted for the events of death and admission to the ICU and for the combined event of death and admission to the ICU. As a preliminary step, each variable was categorized into different levels of risk using the stratum-specific likelihood ratios (SSLRs) method of Peirce and Cornell.12 This method allows to categorize a variable into different risk groups by calculating the probability of having a risk event in each group, using information about the prevalence of the risk event and the sensitivity and specificity of a test in each subgroup. Starting with five strata, having the death event evenly distributed, sST2 was categorized into three risk levels. Three logistic regression models were fitted to predict the death event based on the sST2 risk groups. The first model used covariates with clinical importance (age, sex, D Dimer, Ferritin, Troponin T and IL-6); the second one added sST2 to see its prediction potential, and the third model used the stepwise method with bidirectional elimination to choose the best predictive variables based on the Akaike information criterion (AIC). AIC penalizes models with more parameters, so the best model is the one that explains the most variation using the fewest independent variables (lower AIC scores p c d 3 PRESS Medicina Clínica xxx (xxxx) xxx–xxx re better). In addition to AIC, the likelihood ratio test has been used o compare the models. All statistical analyses were performed with the R, v.4.1 statis- ical software. esults tudy population The study population consisted of 495 patients (53% male), ith a mean age of 57.6 ± 17.6 years and median age of 57.0 and nterquartile range (IQR) of [46.0, 72.0] years (Table 1). Overall, 3.6% of the patients were overweight or obese (BMI > 30 kg/m2), nd 13.4% had some type of lung disease. Their cardiovascular risk actors included arterial hypertension as the most frequent (42.8%), ollowed by hypercholesterolemia (34.3%) and diabetes mellitus 30.4%). Some (18.4%) presented evidence of cardiovascular disease, ith cerebrovascular issues (8.7%) and atrial fibrillation (5.3%) as he most frequent signs (supplemental table 1). At admission, the uration of symptoms was a median of 6 days, and the WHO class core was ≥4 in 58.6% of the patients. eterminants of circulating concentrations of sST2 At admission, the patients’ sST2 concentration was 49.2 ng/mL IQR, 30.5–83.6 ng/mL). Supplemental table 1 shows the clinical haracteristics at admission, according to the quartiles of sST2. A ositive association was observed with male gender (p < 0.001) and lder patient age (p < 0.001). Likewise, higher sST2 concentrations ere observed in the presence of comorbidities, such as arterial ypertension (p < 0.001), type 2 diabetes mellitus (p = 0.025), heart ailure (p = 0.036), cerebrovascular disease (p = 0.042), dementia p = 0.032) and chronic obstructive pulmonary disease (p = 0.010). onversely, a significant association was also found between higher evels of sST2 and higher concentrations of markers of stress and eart damage (NT-proBNP and hs-cTnT), inflammation (IL-6, Fer- itin, hs-CRP), and thrombosis (D-Dimer). An association was observed between sST2 concentrations nd clinical severity at admission, in terms of WHO class score p < 0.001), respiratory rate (p = 0.004), and oxygen saturation mea- ured in the emergency room (p < 0.001). ST2 concentrations and in-hospital evolution After admission, patients were followed during their time of ospitalization (median: 6 days, IQR: 3–8 days), and clinical and espiratory worsening, as well as adverse events, were recorded. uring hospital admission, 45 patients died (9.1%), and 46 required dmissions to the ICU (9.3%). As shown in Table 2, the sST2 levels at admission showed a sig- ificant association with a worse hospital evolution, with higher ates of clinical deterioration in terms of the WHO (p < 0.001) and he SOFA (p < 0.001) scales, as well as worsening of oxygen partial ressure (p = 0.001) and oxygen saturation (p < 0.001), need for res- iratory support, development of respiratory distress (p < 0.001), eed for intensive care (p < 0.001), and death (p < 0.001). Furthermore, the sST2 concentrations were higher in patients ho subsequently died (45.6 [IQR; 28.0, 75.9] ng/mL vs. 144 [IQR; 2.6, 319] ng/mL, p < 0.001) and in those requiring ICU admis- ion (44.7 [IQR; 27.5, 71.3] ng/mL vs. 125 [IQR; 69.0, 262] ng/mL, < 0.001), Suplemental Fig. 1. In addition, among the patients who survived and were dis- harged, the presence of pulmonary fibrosis in radiology at ischarge was 24% in patients in the upper quartile at admission, ARTICLE IN PRESSG ModelMEDCLI-6252; No. of Pages 7 M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al. Medicina Clínica xxx (xxxx) xxx–xxx Table 1 Distribution of the clinical characteristics at baseline by quartiles of ST2. sST2 quartiles Overall <30.5 30.6–48 49–83.1 >83.1 p N 495 125 122 123 125 Age, years 57.0 [46.0, 72.0] 49.0 [40.0, 62.0] 53.9 [45.1, 66.0] 62.0 [51.0, 76.0] 64.0 [52.6, 78.0] <0.001 Male 262 (53.0) 42 (33.6) 60 (49.2) 73 (59.8) 87 (69.6) <0.001 BMI, kg/m2 28.9 ± 5.70 27.4 ± 7.36 28.4 ± 3.46 30.5 ± 4.92 28.8 ± 5.70 0.266 Obesity (BMI > 30 kg/m2) 78 (43.6) 17 (38.6) 14 (37.8) 20 (40.8) 27 (55.1) 0.292 Characteristics at admission Lengh of symptoms, days 6 [3, 8] 7 [3, 9] 6 [3, 9] 6 [3, 8] 5 [3, 7] 0.244 Lengh of hospitalization, days 6 [4, 10] 5 [3, 7] 6 [3, 8] 7 [5, 10] 9 [5, 14] <0.001 WHO scale <0.001 <4 192 (41.4) 73 (60.3) 54 (46.6) 37 (31.6) 28 (25.5) ≥4 272(58.6) 48 (39.7) 62 (53.4) 80 (68.4) 82 (74.5) Respiratory rate 19.7 ± 6.14 17.7 ± 5.92 18.2 ± 4.96 20.0 ± 5.09 22.2 ± 7.32 0.004 Sat O2 before O2, % 93.3 ± 6.29 95.7 ± 3.14 94.8 ± 4.26 92.6 ± 5.09 89.7 ± 9.46 <0.001 Sat O2 after O2, % 96.2 ± 2.83 96.6 ± 2.52 96.8 ± 2.15 95.9 ± 2.27 95.7 ± 3.65 0.351 Biomarkers D-dimer, ng/ml 284 [180, 515] 237 [151, 366] 231 [165, 395] 326 [203, 545] 360 [245, 741.3] <0.001 Ferritine, ng/ml 413 [192, 906] 274 [112, 492] 344 [204, 645] 513 [226, 990] 875 [333, 1475] <0.001 IL-6, pg/ml 39.0 [16.2, 69.3] 20.0 [8.30, 34.8] 36.7 [18.8, 60.3] 53.5 [30.8, 81.3] 54.3 [17.3, 117] <0.001 hs-CRP, mg/dl 5.40 [2.37, 11.5] 2.59 [0.91, 4.63] 5.11 [2.02, 9.71] 9.03 [4.12, 15.0] 9.04 [4.38, 15.2] <0.001 hs-c TnT, pg/ml 8.00 [5.00, 17.0] 5.00 [4.00, 9.00] 6.00 [4.00, 12.8] 10.0 [6.00, 18.8] 13.0 [6.00, 34.0] <0.001 NT-proBNP, pg/ml 105 [38.1, 386] 39.9 [16.8, 125] 76.3 [33.0, 196] 159 [61.7, 561] 296 [100, 1672] <0.001 BMI: body mass index; mean ± 83.1 p n 495 125 122 123 125 Adverse clinical events Death 45 (9.10) 2 (1.60) 3 (2.50) 7 (5.70) 33 (26.4) <0.001 Intensive care 46 (9.30) 4 (3.20) 6 (4.90) 4 (3.30) 32 (25.6) <0.001 Death or intensive care 72 (14.5) 6 (4.80) 7 (5.70) 10 (8.10) 49 (39.2) <0.001 WHO scale Worsening* 64 (17.3) 8 (8.20) 8 (8.60) 13 (13.8) 35 (40.7) <0.001 Max <0.001 <4 169 (36.0) 68 (56.7) 47 (40.2) 33 (28.4) 21 (17.9) >4 301 (64.0) 52 (43.3) 70 (59.8) 83 (71.6) 96 (82.1) Maximum SOFA score 2.70 ± 2.76 1.10 ± 1.29 0.86 ± 0.86 2.59 ± 1.23 5.44 ± 3.50 <0.001 Minimum oxygen measures Minimum oxygen pressure 65.5 ± 20.3 80.3 ± 24.4 78.2 ± 17.5 59.7 ± 16.4 55.2 ± 14.4 0.001 Minimum oxygen saturation 91.2 ± 6.54 94.7 ± 3.38 93.6 ± 3.16 88.9 ± 7.64 86.3 ± 7.53 <0.001 Maximal respiratory support required Oxygen therapy glasses 221 (60.2) 43 (43.4) 52 (56.5) 66 (69.5) 60 (74.1) <0.001 Oxygen mask 25 (7.00) 0 (0.00) 4 (4.30) 7 (7.60) 14 (18.4) <0.001 Oxygen high flow 36 (10.1) 3 (3.10) 6 (6.50) 10 (10.9) 17 (23.0) <0.001 ECMO 4 (1.10) 0 (0.00) 0 (0.00) 0 (0.00) 4 (5.30) 0.002 NIV 22 (6.10) 2 (2.00) 2 (2.20) 4 (4.30) 14 (18.2) <0.001 IV 11 (3.00) 0 (0.00) 0 (0.00) 0 (0.00) 11 (13.9) <0.001 Respiratory diseases ARDS development 41 (11.2) 3 (3.10) 4 (4.30) 9 (9.90) 25 (30.5) <0.001 Evidence of pulmonary fibrosis at discharge** 22 (11.7) 1 (2.00) 5 (10.6) 5 (10.6) 11 (24.4) 0.009 ARDS: acute respiratory distress syndrome; ECMO: extracorporeal membrane oxygenation; IV: invasive ventilation; NIV: non-invasive ventilation. mean±SD, median [IQR] s a T sand n (%) * Worsening defined as an increase in OMS scale of a point or more. ** Tested in 168 patients. compared to only 2% in the lower quartile and 10.6% in the inter- mediate quartiles (p = 0.009). Usefulness of sST2 as a predictor of adverse clinical eventsAnalysis of the receiver operating characteristic (ROC) curve showed an area under the curve for death prediction of 0.837 (0.771–0.901), with 90.1% sensitivity and 68.0% specificity at the m d > 4elected cut-off point of 63.3 ng/mL. For prediction of a need for ICU dmission, the area under the ROC curve was 0.762 (0.678–0.847). he selected cut-off point in this case was of 99.9 ng/mL, with sen- itivity 67.4% and specificity 83.5%.Using Peirce and Cornell’s Stratum-specific Likelihood Ratios ethod, three subgroups of sST2 concentrations with significantly ifferent risk were obtained: <82.2 ng/mL, 82.2–210 ng/mL, and 210 ng/mL. Fig. 1 shows the frequency of adverse events and the ARTICLE IN PRESSG ModelMEDCLI-6252; No. of Pages 7 M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al. Medicina Clínica xxx (xxxx) xxx–xxx Fig. 1. The rate of adverse events for each level of ST2. Table 3 Logistic regression models for the endpoint all-cause mortality. Model 1 Model 2 Model 3 Odds ratio (95% CI) p Odds ratio (95% CI) p Odds ratio (95% CI) p Age, per year 1.04 (1.01, 1.08) 0.026 1.05 (1.01, 1.1) 0.011 1.05 (1.01, 1.1) 0.016 Male 1.92 (0.75, 5.39) 0.191 1.48 (0.5, 4.78) 0.490 – – D-dimer, log10 2.07 (0.87, 4.75) 0.089 1.33 (0.53, 3.3) 0.535 – – Ferritin, log10 3.67 (1.4, 10.7) 0.012 2.3 (0.77, 6.96) 0.134 2.76 (0.91, 8.81) 0.075 Hs-cTnT, log10 11.1 (3.3, 38.3) <0.001 9.31 (2.36, 36.7) 0.001 11.3 (2.79, 47.8) 0.001 IL-6, log10 1.37 (0.65, 3.05) 0.429 0.94 (0.44, 2.07) 0.870 – – WHO scale, per unit – – – – 4.03 (1.68, 11.6) 0.004 sST2 risk group – 8 (2, 1 4 (7.46 c a n n t S e s t t r p m t p i m h0–82.2, reference – – 82.2–210 – – 5.9 +210 – – 32. associated risk at each sST2 level. Among the 31 patients with sST2 > 210 ng/mL, the frequencies of adverse events during hospi- talization were 54.8% for death, 48.4% for ICU admission, and 71% for intensive care or death. The corresponding ORs were 39.3 (95% CI: 15.9–103), 23.6 (95% CI: 9.85–58.4), and 38.3 (95% CI: 16.3–97.5) for death, the need for intensive care, and ICU/death, respectively. Table 3 shows the additional value of sST2 measurements for the stratification of risk of death during hospitalization. Consider- ing the statistically significant variables in the prediction of death (Table S1) shown in model 1 (age, gender, D-dimer, ferritin, cardiac Troponin T, and IL-6), the addition of sST2 (model 2) significantly improved the predictive value, as reflected in the reduction of the value of AIC. Model 3, in which the best model was sought using the stepwise method, shows that several variables, including age, WHO classification in the first 24 h, ferritin and troponin on the log- arithmic scale, and categorical sST2, were part of the best model for the prediction of death, with an AIC of 126.89 compared to an AIC of 161.23 and 140.70 of models 1 and 2, respectively. Discussion In this retrospective observational study with a large sample of hospitalized patients with COVID-19, the plasma concentrations of sST2 in the first 24 h of hospitalization showed excellent prognostic value in predicting the risk of clinical worsening and death during hospitalization. i i w d 5– – – 9.6) 0.002 4.81 (1.60, 15.7) 0.006 , 159) <0.001 32.6 (7.48, 162) <0.001 The spread of SARS-CoV-2 around the world has led to a hospital ollapse, which has posed an unprecedented challenge to clinicians nd governments. COVID-19 can be an asymptomatic or mild ill- ess, or it can lead to the onset of SARS, with respiratory failure, eed for respiratory support, and even death in some patients. After two years of this pandemic, multiple waves of infec- ion, and even with vaccination of the majority of the population, ARSCoV-19 continues to pose a health problem. In this scenario, arly identification of patients susceptible to developing more evere symptoms and who will need ventilatory support is essential o improve the individualized treatment of symptomatic patients. sST2 is the soluble form of tumorigenicity suppressor 2 and par- icipates in the IL-33/ST2 axis, which is involved in the immune esponse to viruses, as well as in autoimmune diseases, asthma, and ulmonary fibrosis. As our group has previously demonstrated, pul- onary pneumocytes are the main producers of sST2 in response o alveolar damage.11 Abers et al. analyzed a large number of inflammation-related roteins to characterize the immune response to COVID-19, ncluding the activation of different immune cells, such as onocytes/macrophages, neutrophils, T lymphocytes, and non- ematopoietic cells. In their study, sST2 levels, along with other nflammatory markers, such as sTNFRSF1A, IL-10, and IL-15, were ncreased in COVID-19 patients and showed a strong correlation ith disease severity, with marked elevations in patients who ied.13 ING Model b p o A S s E V C F C A a M M M a d A f 2 RARTICLEMEDCLI-6252; No. of Pages 7 M. Arnaldos-Carrillo, J.A. Noguera-Velasco, I.M. Martínez-Ardil et al. The usefulness of the sST2 biomarker as a predictor of severity in patients with COVID-19 has already been studied by other research groups, although the number of patients included in these studies was smaller than ours. Sánchez-Marteles et al. studied the con- centration of sT2 at admission and discharge in 144 patients, 14 of whom required admission to the ICU (9.7%) and 1 who died (0.7%).14 Omland et al. studied 123 patients, of whom 27 were admitted to the ICU (22%) and 8 died (6.5%).15 Alladina et al. studied 72 patients who were admitted to the ICU and 77 patients who were not admit- ted to the ICU but required oxygen supplementation.16 The average age of the patients in those studies was similar to ours, as was the presence of comorbidities. In our study, similar to the findings in previous studies,14,15 we found a good association between sST2 levels and markers of inflammation, such as hs-CRP or IL-6, and between sST2 and other acute phase reactants, such as ferritin and D-dimer. Several studies conducted in recent years have shown sST2 to be very useful in determining the severity and prognosis in patients with heart failure.17 In our analysis, we also found an association between sST2 levels in patients with COVID-19 and markers of stress and heart damage, such as NT-proBNP and tro- ponin T. Increases in Troponin T have also been associated with the severity of COVID infections.18,19 Omland obtained similar results15 whereas Sánchez-Marteles did not study cardiac markers.14 sSt2 has a pulmonary origin, and we have observed a statis- tically significant association between the levels of this biomarker and the oxygen saturation of patients at admission, as also reported by Omland et al., as well as with the WHO class during the first 24 h of hospitalization.15 This agrees with the findings of Allad- ina et al., who observed that the plasma sST2 concentrations were associated not only with the severity of the disease, but also with the need for respiratory support and, consequently, with persistent lung damage.16 Our analysis of the ROC curves for the prognosis of ICU admis- sion and death showed excellent performance, with an AUC of 0.762 for the case of need for intensive care and an AUC of 0.836 for the prognosis of death. These values are similar to those recently pub- lished, although those studies all included fewer patients.14,15,20,21 The previous studies indicated that age and gender, together with inflammatory markers and heart damage, were clear predictors of risk in patients with COVID-19,22–24 similary to our own results (Supplemental table 2). In our study, we evaluated different risk models that integrate different biomarkers (D-dimer, ferritin, car- diac Troponin T, and IL-6), age, and gender, as well as the clinical status of the patients in terms of their WHO class. In all cases, sST2 provided additional information on these risk variables and is therefore postulated to be a powerful tool for better management of patients with COVID-19. The main limitation of the present study is that the patients came from two different waves of infection and, in an epidemio- logical situation, with different variables than in the current wave. However, the robustness of the findings supports the value of sST2 as a marker of risk in the symptomatic COVID-19 patient, although a prospective evaluation in new cohorts would be rec- ommended in the coming years. Another limitation is the absence of serial values of sST2 during hospitalization, since the concentra- tions were measured only at admission. Our hypothesis is that the serial determination of sST2 values throughout a patient’s hospi- talization could be of additional benefit in monitoring the risk of disease progression and response to treatment, especially consid- ering the current new treatment options. In conclusion, this study supports the usefulness of sST2 and the measurement of its circulating concentrations for assessment of the risk of clinical and respiratory deterioration, as well as serious complications, in patients with symptomatic COVID-19 disease and particularly those patients requiring hospital care. Therefore, this 6 PRESS Medicina Clínica xxx (xxxx) xxx–xxx iomarker may become an important tool to help identify at-risk atients who may benefit from closer follow-up or even initiation f specific therapies to endeavor to prevent disease progression. uthors’ contributions MAC: Investigation, Methodology, Writing – original draft. JANV: Writing – original draft, Methodology, Conceptualization, upervision. IMMA: Investigation, Methodology. ARP: Formal analysis, Data curation. ICL: Investigation, Methodology. AHV: Formal analysis, Data curation. JARL: Investigation. AK: Writing – review & editing. ABG: Writing – review & editing. DPF: Writing – original draft, Conceptualization, Funding acqui- ition, Supervision. thical considerations Informed consent was obtained from all patients as well as the irgen de la Arrixaca University Hospital Clinical Research Ethics ommittee (CEIm), internal code: 2020-6-1-4HCUVA. unding None. onflict of interest None of the authors have any conflict of interest. cknowledgments We are grateful for the generous contribution of the patients nd the collaboration of the Biobank Network of the Region of urcia, BIOBANC-MUR, registration number B.0000859. BIOBANC- UR, supported by the “Instituto de Salud Carlos III”, “Instituto urciano de Investigación Biosanitaria Virgen de la Arrixaca, IMIB” nd “Consejería de Salud de la Comunidad Autónoma de la Región e Murcia.” ppendix A. Supplementary data Supplementary data associated with this article can be ound, in the online version, at http://dx.doi.org/10.1016/j.medcli. 023.04.005 eferences 1. 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