------------------- GENERAL INFORMATION ------------------- 1. Dataset title: Raw and Processed Data Supporting “Enhancing ADCC Responses in HIV-1 Vaccines: CD4 Incorporation into Virus-Like Particles” 2. Contact Name: Eloisa Yuste Filiation: National Microbiology Center. Instituto de Salud Carlos III, 28220 Madrid, Spain / Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), 28222 Madrid, Spain email: eyuste@isciii.es ORCID: 0000-0002-9484-9974 3. Project description Generation and characterization of vaccine prototypes based on HIV-1 VLPs with improved accesibility to CD4 inducible epitopes. 4. Dataset description This dataset includes the raw data included in the publication. 5. Notes 6. Date of file deposit: 11/26/2025 7. File creation date: S1: 10/14/2025 S2: 10/16/2025 S3: 10/16/2025 S4: 10/16/2025 S5: 10/16/2025 8. Language: English -------------------------- AUTHORSHIP -------------------------- 1. Authors: First name: Almudena Last name: Rubio-Perez (First autor) Filiation: National Microbiology Center. Instituto de Salud Carlos III, 28220 Madrid, Spain. e-mail: arubio@isciii.es ORCID: 0009-0006-4687-7555 First name: Miguel Last name: Martin-Serrano Filiation: National Microbiology Center. Instituto de Salud Carlos III, 28220 Madrid, Spain / Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), 28222 Madrid, Spain e-mail: miguelmartinserrano99@gmail.com ORCID: 0000-0002-9220-4566 First name: Félix Last name: Docando Filiation: Electron Microscopy Unit, Instituto de Salud Carlos III, 28220 Madrid, Spain e-mail: f.docando@isciii.es ORCID: 0000-0001-8835-9349 First name: María C. Last name: Terrón Filiation: Electron Microscopy Unit, Instituto de Salud Carlos III (ISCIII), 28220 Madrid, Spain e-mail: mcterron@isciii.es ORCID: 0000-0001-9072-8158 First name: Victor Last name: Sanchez-Merino (corresponding) Filiation: National Microbiology Center. Instituto de Salud Carlos III, 28220 Madrid, Spain / Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), 28222 Madrid, Spain e-mail: vmsanchez@isciii.es ORCID: 0000-0001-9400-427X First name: Eloisa Last name: Yuste (corresponding) Filiation: National Microbiology Center. Instituto de Salud Carlos III, 28220 Madrid, Spain / Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), 28222 Madrid, Spain e-mail: eyuste@isciii.es ORCID: 0000-0002-9484-9974 -------------------------- METHODS -------------------------- 1. Methods: S1: Nanoparticle Tracking Analysis (NTA) S2: Western-blotting S3: ELISAs S4: ELISAs S5: FcγRIIIa reporter assay 2. Software S1: Nanosight NS300 NTA software (version 3.4) S2: Excel S3: Excel S4: Excel S5: Excel -------------------------- KEYWORDS AND SUBJECTS -------------------------- 1. Keywords: HIV-1, Vaccines, Virus-like particles, ADCC, Antibodies -------------------------- SPONSOR INFORMATION AND GRANT IDENTIFIERS -------------------------- 1. Grant information: This work was supported by Instituto de Salud Carlos III (ISCIII) through the grant CIII/00039, co-funded by the European Regional Development Fund (ERDF/FEDER) and through the grant PID2023-148729OB-I00 funded by MICIU/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”. A.R.P. was supported by the Comunidad de Madrid through the Plan de Empleo Juvenil 2023 (PEJ-2023-AI/SAL-GL-28125), co-financed by the European Social Fund Plus (FSE+). -------------------------- RELATED PUBLICATIONS -------------------------- 1. Haynes, B.F., Gilbert, P.B., McElrath, M.J., Zolla-Pazner, S., Tomaras, G.D., Alam, S.M., et al. (2012). Immune-correlates analysis of an HIV-1 vaccine efficacy trial. N Engl J Med 366(14), 1275-1286. doi: 10.1056/NEJMoa1113425. 2. Envelope Glycoproteins from Diverse Clades Differentiate Antibody Responses and Durability among Vaccinees. J Virol 92(8). doi: 10.1128/JVI.01843-17. 3. Veillette, M., Coutu, M., Richard, J., Batraville, L.A., Dagher, O., Bernard, N., et al. (2015). The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals. J Virol 89(1), 545-551. doi: 10.1128/JVI.02868-14. 4. Sanchez-Merino, V., Fabra-Garcia, A., Gonzalez, N., Nicolas, D., Merino-Mansilla, A., Manzardo, C., et al. (2016). Detection of Broadly Neutralizing Activity within the First Months of HIV-1 Infection. J Virol 90(11), 5231-5245. doi: 10.1128/JVI.00049-16. 5. Beltran-Pavez, C., Ferreira, C.B., Merino-Mansilla, A., Fabra-Garcia, A., Casadella, M., Noguera-Julian, M., et al. (2018). Guiding the humoral response against HIV-1 toward a MPER adjacent region by immunization with a VLP-formulated antibody-selected envelope variant. PLoS One 13(12), e0208345. doi: 10.1371/journal.pone.0208345. -------------------------- GEOGRAPHIC INFORMATION -------------------------- 1. Geographic coverage -------------------------- TEMPORARY INFORMATION -------------------------- 1. Temporary coverage -------------------------- FILES -------------------------- S1: NTA data obtained with Nanosight NS300 NTA software (version 3.4. PDF file. English. S2: Western-blotting. Bands quantification. Excel. English. S3: ELISA raw data and Area under the curve (A.U.C.). Excel. English. S4: ELISA raw data and Area under the curve (A.U.C.). Excel. English. S5: FcγRIIIa reporter assay raw data. Excel. English -------------------------- USAGE RIGHTS AND PRIVACY -------------------------- 1. Usage licenses CC BY 4.0 2. Privacy -------------------------- OTHERS -------------------------- 1. 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