LOCUS loxP-myctagBFP-STOP-lox-mCherryNTR fl Kan 7515 bp ds-DNA circular SYN 30-AGO-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Extended Data File 4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7515) AUTHORS . TITLE Direct Submission JOURNAL Exported martes, 30 de ago de 2016 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7515 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 432..465 /label=LoxP /note="LoxP" misc_feature 466..472 /label=kozak /note="kozak" misc_feature 473..475 /label=ATG /note="ATG" misc_feature 476..505 /label=myc /note="myc" misc_feature 506..1225 /label=tagBFP /note="tagBFP" misc_feature 1226..1729 /label=Rabbit beta globin terminator /note="Rabbit beta globin terminator" misc_feature 1730..1763 /label=LoxP(1) /note="LoxP(1)" misc_feature 1778..1784 /label=kozak(1) /note="kozak(1)" misc_feature 1785..2493 /label=mCherry /note="mCherry" misc_feature 2494..2543 /label=Linker /note="Linker" misc_feature 2544..3197 /label=NTR /note="NTR" misc_feature 3216..3454 /label=SV40 poliA /note="SV40 poliA" misc_feature 3491..3525 /label=FRT /note="FRT" misc_feature 3780..3918 /label=Sv40 poliA /note="Sv40 poliA" misc_feature 3969..4761 /label=KanR /note="KanR" misc_feature 4842..4876 /label=FRT(1) /note="FRT(1)" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctcggcgcg cccgggaccg 421 gtggatccac tataacttcg tatagcatac attatacgaa gttatcgcca ccatggagca 481 gaagctgatc tcagaggagg acctgatgag cgagctgatt aaggagaaca tgcacatgaa 541 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg 601 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc 661 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca 721 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag 781 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga 841 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt 901 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg 961 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc 1021 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt 1081 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga 1141 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct 1201 taattaaagc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt 1261 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg 1321 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat 1381 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa 1441 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct 1501 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc 1561 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg 1621 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag 1681 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctca taacttcgta 1741 tagcatacat tatacgaagt tatcggatcc catcgatcgc caccatggtg agcaagggcg 1801 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 1861 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 1921 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 1981 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 2041 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 2101 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 2161 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 2221 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 2281 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 2341 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 2401 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 2461 actccaccgg cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt 2521 cgaattcagg cggcggcgcc gccatggaca tcatctctgt ggctctgaag agacatagca 2581 caaaggcttt tgatgccagc aagaaactga cccctgagca ggctgagcag atcaagacac 2641 tgctgcagta cagcccaagc agccagaaca gccagccttg gcattttatt gtggcttcta 2701 cagaggaggg aaaggctaga gtggctaagt ctgctgctgg aaattatgtg ttctctgaga 2761 gaaagatgct ggatgcctct catgtggtgg tgttctgtgc taagacagcc atggatgatg 2821 tgtggctgaa actggtggtg gaccaggagg atgctgatgg aagatttgcc acacctgagg 2881 ctaaggctgc taatgataag ggaagaaagt tcacagctga tatgcacaga aaggacctgc 2941 atgatgatgc tgagtggatg gctaagcagg tgtatctgaa tgtgggaaac ttcctgctgg 3001 gagtggctgc tctgggactg gatgctgtgc ctattgaggg atttgatgct gctatcctgg 3061 atgctgagtt tggactgaag gagaagggat acaccagcct ggtggtggtg ccagtgggac 3121 accactctgt ggaggatttt aatgctacac tgcctaagag cagactgcct cagaacatca 3181 ccctgacaga ggtgtaagag ctcgaattcg cccttaggcc tgcaggatca taatcagcca 3241 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 3301 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 3361 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 3421 ttgtggtttg tccaaactca tcaatgtatc ttaaactagg ggggatctgc tagcagataa 3481 ctgatcagct tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca 3541 agatccccct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 3601 tttcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 3661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 3721 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg 3781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3841 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3901 gcatctcaat tagtcagtaa ccatagtccg caaagatcga tcaagagaca ggatgaggat 3961 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 4021 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 4081 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 4141 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 4201 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 4261 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 4321 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 4381 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 4441 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 4501 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 4561 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 4621 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 4681 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 4741 gccttcttga cgagttcttc tgaggggatc ggcaataaaa agacagaata aaacgcacgg 4801 gtgttgggtc gtttgttcgg atccgagctt caaaagcgct ctgaagttcc tatactttct 4861 agagaatagg aacttcggaa tagtaacttc tccatggtag cctccaaaaa agcctcctca 4921 ctacttctgg actagtgcgg ccgcgcgttt aagtggcctg tgactacgca gtgtcattat 4981 cttacagaat aggtttggac ccagatgttg tggtgcctgg aggttttaaa ttttggaaag 5041 cccatctcag gaaaaaaaaa aaccacaaaa ttatgaatac agaggtgttg ggctgcccct 5101 gggacttgaa agggagcttg taatttgggg accagaggtt taaatcccta tagtttcact 5161 gtgaattatt caccttgctc aaactttcac tctgagaggc tgcctgatta ggtgcctagc 5221 gagaccagct ggatgtagct ttgtgctctt tcctctgttg attttctccg cttaattaag 5281 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 5341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 5401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 5461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 5521 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 5581 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 5641 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 5701 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 5761 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 5821 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 5881 cgctttctca aagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 5941 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6001 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6061 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6121 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6181 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 6241 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 6301 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 6361 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 6421 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 6481 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 6541 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 6601 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 6661 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 6721 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 6781 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 6841 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 6901 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6961 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7021 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7081 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7141 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7201 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 7261 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 7321 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 7381 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 7441 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 7501 cgaggccctt tcgtc //