LOCUS Tol2_Amp_Cryst_RFP 7724 bp ds-DNA circular SYN 30-AGO-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Extended Data File 3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7724) AUTHORS . TITLE Direct Submission JOURNAL Exported martes, 30 de ago de 2016 from SnapGene Viewer 3.0.3 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7724 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 683..706 /label=pTarBAC_HA1_iTol2_fw /note="pTarBAC_HA1_iTol2_fw" misc_feature complement(702..901) /label=Inverted L200 /note="Inverted L200" misc_feature complement(917..935) /label=amp_HA1_control_rev /note="amp_HA1_control_rev" misc_feature 1042..1902 /label=AmpR /note="AmpR" misc_feature 1868..1887 /label=amp_HA2_control_fw /note="amp_HA2_control_fw" misc_feature 1925..2074 /label=Inverted R150 /note="Inverted R150" primer_bind 2129..2148 /label=T3 /note="T3" promoter 2149..4387 /label=gamma Crys promoter /note="gamma Crys promoter" CDS 4397..5107 /label=mCherry /note="mCherry" polyA_signal 5149..5340 /label=SV40 late polyA /note="SV40 late polyA" misc_feature 5304..5324 /label=cryst_HA2_control_fw /note="cryst_HA2_control_fw" misc_feature complement(5338..5361) /label=pTarBAC_HA2_iTol2CrystRFP_rev /note="pTarBAC_HA2_iTol2CrystRFP_rev" primer_bind complement(5388..5407) /label=T3(1) /note="T3(1)" misc_feature 5388..5407 /label=ATTAACCCTCACTAAAGGGA /note="ATTAACCCTCACTAAAGGGA" misc_feature 5410..5416 /label=AttB1 /note="AttB1" misc_recomb 5417..5566 /label=AttR1 /note="AttR1" misc_recomb 5417..5566 /label=AttP1 /note="AttP1" misc_recomb 5417..5430 /label=AttL1 /note="AttL1" misc_feature 5417..5430 /label=AttB1(1) /note="AttB1(1)" misc_feature 5417..5423 /label=Gateway recomb1 /note="Gateway recomb1" misc_feature 5971..6981 /label=SpectR /note="SpectR" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gaattcgccc ttccctgctc gagccgggcc caagtgatct ccaaaaaata 721 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa 781 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc 841 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct 901 ggttcttgac cccctaccac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 1021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 1081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 1141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 1201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 1261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 1321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 1381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 1441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 1501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 1561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 1621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 1681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 1741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 1801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 1861 cagatcgctg agataggtgc ctcactgatt aagcattggt aaggtaccgg catatggttc 1921 ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg 1981 gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta 2041 aaaaagtact ccattaaaat tgtacttgag tattagatcg tggcggccgc tctagaacta 2101 gagtcgagat ccaagcttgg ggcgcgcaat taaccctcac taaagggaac aaaagctggt 2161 accgggcccc ccctcgagtc cataagaaag atataaataa gctggagaga atgcagaatt 2221 gtcaactagc agtgctggaa ttggggaggg atgccatccc ctcacttttt tatttctgtg 2281 aaatagcatc ccctcagtgt ggatgcaagt tttttgaatc ccggaactgt cttttgctcc 2341 tcttcacttc tgcatgcaaa tacaatgcag aggtaaaata taatatagtt aaatatatag 2401 gggattaaag gagagaaaaa attgctggga gagaagaaga aaagatagag aagtgttaaa 2461 cagataaaga aggtggaggc atcagagagg gttatagaga aaaggagaga agaaaaaatg 2521 aaggtattaa acagcagcag attaaggaat agtgagaaaa tgctttatat gaaataatct 2581 tgggtaatat agaatatgat ggggatgtgc tatgttatga cacaatacac agaaaaggtg 2641 ctttatttgc agtagctgaa ttggtagcca agaaaagtca taagcacaat ttttattttg 2701 gggtccctat aggtcacata tgtaaatatg gttatgggag ggatgcacca ctcagagcat 2761 ccctccactt tttttactcc aattcaagca ctgggaatga gatatgcttc attagcacct 2821 aaacatttga caatgaccaa tgtgaatgtt agcgaccata tttgcgtcct tttgtgacca 2881 aatggcatat gtgaacattc ctagtgtatg taataaaatt ttgcaacaaa atatttaaaa 2941 actccagagc agatgcttaa cttagtcatg taatattcgc cagcaaatga gtatacctct 3001 atttttaaca tcacttgtga tttttttagt aacattccca cctttagtat ttccagattg 3061 aaattatcat acccccccat tctttaatat agggaacact gtaccagtgg gtctgggatt 3121 atttgtccat atattgcaat atatccaagc tgaccaactg tctcaaagtc acccctggtc 3181 tggccaataa aacacaaaca ttctagcaga aagtaaagct ggccatagat gttgagattt 3241 ttaaaagatc agatcctgat cgtgagacca cgatcttctc agaacgatcg tacgatcgta 3301 cgaatctacc atcaactaaa aagaccaatt taccaggaaa acaaagggga gctgcctgct 3361 tggccctgca aacatagaga gattgcactg gggccgacaa agattttttg acctggccga 3421 tcaatttccc gacagatgtc ggccgaaaaa tcgtaagatg tacgatcgtt cgaataccac 3481 taaccgcacg ataatttcga aggattggtc gggcttccct aaaatcggtc gttcggcaag 3541 aagaatcgtc gcgtctatgg ggaccttaag tgtacttgct ttccaaggtt aaaaattgtt 3601 acctttttat acgacactgc atggatcacc tgaaagcagg ttgggagcta caacatggaa 3661 ctagccactg ttcctttata tggttatcac tacccaattt taaataatta aactggagtt 3721 tattgatggt gtgaccacaa aattgataca gataaagaca tactgtatat attgcgttat 3781 atgacaaata cagtaacacc acattactat tttaaattat atataaaata atattaatat 3841 atctggggac ataaaatgtg gcttcttaaa cattggggtg acactgatga gattatttgg 3901 taaatgtttg atgctagcta accattgttt ggttcatttg tgtgattcaa aagtggcaaa 3961 gaatgacacc tagatgatgc caaattaagg atttactagt aaccacaatt ctttaaactg 4021 ttacaaagtg tttggaccag caactgaatt gcactgtaaa ttaccacatc agggtctccg 4081 gggtcaacgc tgctaagcaa tggactggaa aatttgtgac attgagtttt tggaataact 4141 gggaatgctg gataactctt tgggtaaagc tatggaacaa aagtaccagc atgaactaag 4201 aaatgactgt cacagcacaa catcaaacac aatgcaagac aaaggttctc tgcttttgcc 4261 ttacttgaca gaaaaagtaa gctctaggtt catcgcatgc agacagcagc aatacatgac 4321 atatataaag gctggctctt ttctcccttc cattgaactg aaacttccac tcagtcagac 4381 ttgcggggga tccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 4441 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 4501 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 4561 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 4621 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 4681 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 4741 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 4801 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 4861 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 4921 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 4981 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 5041 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 5101 caagtaactc gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa 5161 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt 5221 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta 5281 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt 5341 aatcgatacc gtcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag 5401 ggttaattgc aagtttgtac aaaaaagttg aacgagaaac gtaaaatgat ataaatatca 5461 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 5521 tgcagtcact atgaatcaac tacttagatg gtattagtga cctgtagaat tgatctagag 5581 gatcataata agggcgaatt cgacccagct ttcttgtaca aagttggcat tataaaaaat 5641 aattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt 5701 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 5761 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 5821 tcatgcctcc tctagaccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt 5881 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt 5941 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg 6001 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt 6061 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga 6121 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag 6181 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt 6241 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca 6301 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg 6361 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct 6421 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt 6481 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt 6541 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa 6601 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt 6661 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc 6721 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca 6781 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg 6841 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat 6901 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc 6961 accaaggtag tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt 7021 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 7081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 7141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 7201 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 7261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 7321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 7381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 7441 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag 7501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 7561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 7621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 7681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt //