Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/9968
Título
Flow Cytometry Has a Significant Impact on the Cellular Metabolome
Autor(es)
Binek, Aleksandra CNIC | Rojo, David | Godzien, Joanna | Rupérez, Francisco Javier | Nunez, Vanessa CNIC | Jorge, Inmaculada CNIC | Ricote, Mercedes CNIC | Vazquez, Jesus CNIC | Barbas, Coral
Fecha de publicación
2019-01
Cita
J Proteome Res. 2019; 18(1):16981
Idioma
Inglés
Tipo de documento
journal article
Resumen
The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyze the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signaling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry.
Palabras clave
CE−MS | GC−MS | LC−MS | Fluorescence activated cell sorting (FACS) | Metabolome profile | Metabolomics | Multiplatform analysis | Sorted cells | Sorting
MESH
Animals | Cells, Cultured | Flow Cytometry | Macrophages, Peritoneal | Mice | Research Design | Cell Separation | Metabolome
Versión en línea
DOI
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