Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/9090
Título
Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis virulence in the non-conventional host Galleria mellonella
Autor(es)
Gago Prieto, Sara ISCIII | Garcia-Rodas, Rocio ISCIII | Cuesta de la Plaza, Isabel ISCIII | Mellado, Emilia ISCIII | Alastruey-Izquierdo, Ana ISCIII
Fecha de publicación
2014-02-15
Cita
Virulence. 2014 Feb 15;5(2):278-85.
Idioma
Inglés
Tipo de documento
journal article
Resumen
The incidence of fungal infections due to C. parapsilosis and closely related cryptic species (-psilosis complex) has increased in the last few years, but differences in virulence among these species have not been widely studied. Fifteen clinical isolates of C. parapsilosis, C. orthopsilosis, and C. metapsilosis, including the type strains, were used to evaluate their virulence in Galleria mellonella larvae. Fluctuations in the hemocyte density and in the phagocytic activity were also tested. Differences in the median survival for these species were demonstrated at 37 °C (2.6 ± 1.02, 2.3 ± 0.92, and 4.53 ± 1.65 d for C. parapsilosis, C. orthopsilosis, and C. metapsilosis, respectively). Galleria mellonella hemocytes phagocytosed C. metapsilosis strains more effectively than did for C. orthopsilosis and C. parapsilosis (P<0.05). The phagocytosis rate was lower for C. parapsilosis than for C. orthopsilosis (P<0.05). The hemocyte density was increased in larvae infected with C. metapsilosis compared with those infected with C. parapsilosis or C. orthopsilosis (P<0.05). Moreover, in vitro studies of virulence factors such as pseudohyphae production and hydrolytic enzyme secretion showed that the capability of C. metapsilosis strains to produce those virulence factors was reduced. Infections due to -psilosis complex species produced tissue damage in G. mellonella and pseudohyphae could be also observed during infection with C. parapsilosis.
Palabras clave
Candida metapsilosis | Candida orthopsilosis | Candida parapsilosis | Galleria mellonella | Virulence
MESH
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