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XTEND: Extending the depth of field in cryo soft X-ray tomography

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XTENDExtendingTheDepth_2017.pdf (3.53 MB)
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URI: http://hdl.handle.net/20.500.12105/7365
ISSN: 2045-2322
DOI: 10.1038/srep45808

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2017-04-04

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Abstract

We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.

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Algorithms Image Processing, Computer-Assisted Imaging, Three-Dimensional Tomography, X-Ray

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Sci Rep. 2017 Apr;7:45808

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Centro Nacional de Microbiología (CNM)

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https://doi.org/10.1038/srep45808

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research article