Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/6634
Título
Molecular cloning and characterisation of the RESA gene, a marker of genetic diversity of Plasmodium falciparum
Autor(es)
Gonzalez, Luis Miguel ISCIII | Cuevas, Laureano ISCIII | Perez-Pastrana, Esperanza ISCIII | Santa-María, Ysmael | Benito, Agustin ISCIII
Fecha de publicación
2009-10-09
Cita
Mol Biol Rep. 2010;37(6):2893-902.
Idioma
Inglés
Tipo de documento
journal article
Resumen
To identity immunodiagnostic antigen genes, a Plasmodium falciparum (Dd2 clone) expression library was screened using human immune sera. The ring-infected erythrocyte surface antigen (RESA) was isolated: this antigen of the resistant clone presents repeat tandem sequences like the 3D7 clone, albeit in different numbers. RESA has been studied as a marker of genetic diversity, with different sizes being observed in different isolates and clones of Plasmodium falciparum. The native protein was localised in cultures by western-blot and immuno-transmission electron microscopy. The antigenicity of RESA was evaluated by ELISA, using the carboxy-terminal repeat region as antigen. The assay's sensitivity and specificity were 78.2 and 94% respectively.
Palabras clave
MESH
Amino Acid Sequence | Antigens, Protozoan | Blotting, Western | Cell Extracts | Cloning, Molecular | Computational Biology | DNA, Complementary | Genetic Markers | Humans | Molecular Sequence Data | Plasmodium falciparum | Protein Transport | Protozoan Proteins | Recombinant Fusion Proteins | Sequence Alignment | Genetic Variation
Versión en línea
DOI
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