Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/6514
Título
Lamin A/C augments Th1 differentiation and response against vaccinia virus and Leishmania major
Autor(es)
Toribio-Fernandez, Raquel CNIC | Zorita, Virginia CNIC | Rocha-Perugini, Vera CNIC | Iborra, Salvador CNIC | Martinez del Hoyo, Gloria CNIC | Chevre, Raphael CNIC | Dorado, Beatriz CNIC | Sancho, David CNIC | Sanchez-Madrid, Francisco CNIC | Andres, Vicente CNIC | Gonzalez-Granado, Jose Maria CNIC
Fecha de publicación
2018
Cita
Cell Death Dis. 2018; 9(1):9
Idioma
Inglés
Tipo de documento
journal article
Resumen
Differentiation of naive CD4(+) T-cells into functionally distinct T helper (Th) subsets is critical to immunity against pathogen infection. Little is known about the role of signals emanating from the nuclear envelope for T-cell differentiation. The nuclear envelope protein lamin A/C is induced in naive CD4(+) T-cells upon antigen recognition and acts as a link between the nucleus and the plasma membrane during T-cell activation. Here we demonstrate that the absence of lamin A/C in naive T-cell reduces Th1 differentiation without affecting Th2 differentiation in vitro and in vivo. Moreover, Rag1(-/-) mice reconstituted with Lmna(-/-)CD4(+)CD25(-) T-cells and infected with vaccinia virus show weaker Th1 responses and viral removal than mice reconstituted with wild-type T-cells. Th1 responses and pathogen clearance upon Leishmania major infection were similarly diminished in mice lacking lamin A/C in the complete immune system or selectively in T-cells. Lamin A/C mediates Th1 polarization by a mechanism involving T-bet and IFN gamma production. Our results reveal a novel role for lamin A/C as key regulator of Th1 differentiation in response to viral and intracellular parasite infections.
Palabras clave
CD8(+) T-CELLS | CUTANEOUS LEISHMANIASIS | DENDRITIC CELLS | TRANSCRIPTION FACTOR | LINEAGE COMMITMENT | NUCLEAR-ENVELOPE | C57BL/6 MICE | C-FOS | IMMUNITY | EFFECTOR
Versión en línea
DOI
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- Nombre:
- LaminA-CAugmentsTh1_2018_SuplM ...
- Tamaño:
- 18.51Kb
- Formato:
- Microsoft Word 2007