Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/10870
Título
Molecular Characterization and Antimicrobial Susceptibilities of Nocardia Species Isolated from the Soil; A Comparison with Species Isolated from Humans
Autor(es)
Carrasco, Gema ISCIII | Monzon-Fernandez, Sara ISCIII | San Segundo, Maria ISCIII | García, Enrique | Garrido, Noelia ISCIII | Medina-Pascual, Maria Jose ISCIII | Villalon-Panzano, Pilar ISCIII | Ramírez, Ana | Jimenez Sancho, Maria Pilar ISCIII | Cuesta de la Plaza, Isabel ISCIII | Valdezate, Sylvia ISCIII
Fecha de publicación
2020-06-15
Cita
Microorganisms. 2020 Jun 15;8(6):900.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Nocardia species, one of the most predominant Actinobacteria of the soil microbiota, cause infection in humans following traumatic inoculation or inhalation. The identification, typing, phylogenetic relationship and antimicrobial susceptibilities of 38 soil Nocardia strains from Lara State, Venezuela, were studied by 16S rRNA and gyrB (subunit B of topoisomerase II) genes, multilocus sequence analysis (MLSA), whole-genome sequencing (WGS), and microdilution. The results were compared with those for human strains. Just seven Nocardia species with one or two strains each, except for Nocardia cyriacigeorgica with 29, were identified. MLSA confirmed the species assignments made by 16S rRNA and gyrB analyses (89.5% and 71.0% respectively), and grouped each soil strain with its corresponding reference and clinical strains, except for 19 N. cyriacigeorgica strains found at five locations which grouped into a soil-only cluster. The soil strains of N. cyriacigeorgica showed fewer gyrB haplotypes than the examined human strains (13 vs. 17) but did show a larger number of gyrB SNPs (212 vs. 77). Their susceptibilities to antimicrobials were similar except for beta-lactams, fluoroquinolones, minocycline, and clarithromycin, with the soil strains more susceptible to the first three (p ≤ 0.05). WGS was performed on four strains belonging to the soil-only cluster and on two outside it, and the results compared with public N. cyriacigeorgica genomes. The average nucleotide/amino acid identity, in silico genome-to-genome hybridization similarity, and the difference in the genomic GC content, suggest that some strains of the soil-only cluster may belong to a novel subspecies or even a new species (proposed name Nocardia venezuelensis).
MESH
MLSA | Nocardia cyriacigeorgica | Nocardia spp | WGS | Antimicrobial resistance | gyrB | Nocardiosis | Potential new species/subspecies N. venezuelensis | Soil
Versión en línea
DOI
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